Lipid production by oleaginous Mucorales cultivated on renewable carbon sources

Mortierella isabellina and Cunninghamella echinulata were cultivated on glucose-, pectin-, starch- and lactose-based media. Culture on glucose at two initial C/N ratios favored lipid synthesis in the media with increased C/N. Starch was an adequate substrate for both molds, but lipid (in g/g of biomass) was produced in lower quantities compared with the glucose trial. Pectin was inadequate for C. echinulata whereas growth of M. isabellina was satisfactory (8.4 g/L), followed by moderate lipid production. Growth of C. echinulata on lactose was negligible, while that of M. isabellina was notable (9.5 g/L) although lipid in biomass was only 0.36 g/g. Hydrolytic enzymes (α-amylase, polygalacturonase and β-galactosidase) activities of both strains seemed to be low enough to saturate their metabolic capabilities. This seemed a major cause for the lower amount of lipid accumulated during growth on complex media compared with that on glucose. Cellular fatty acids of M. isabellina were oleic, linoleic and palmitic acid, while γ-linolenic acid (GLA) was produced in low quantities. In C. echinulata grown on glucose, lactose or starch, GLA concentration was notable at the beginning and end of culture. Growth on pectin at the first growth steps was accompanied by the production of saturated fatty acids, the amount of which decreased thereafter.

[1]  S. Papanikolaou,et al.  Selective uptake of fatty acids by the yeast Yarrowia lipolytica , 2003 .

[2]  B. Macris.  Production of a Thermostable β-d-Galactosidase by Alternaria alternata Grown in Whey , 1982, Applied and environmental microbiology.

[3]  S. Narine,et al.  Implications for the use of Mortierella fungi in the industrial production of essential fatty acids , 2005 .

[4]  Seraphim Papanikolaou,et al.  Compositional shifts in lipid fractions during lipid turnover in Cunninghamella echinulata , 2007 .

[5]  M. Komaitis,et al.  Lipid and γ-linolenic acid accumulation in strains of zygomycetes growing on glucose , 2001 .

[6]  J. Nicaud,et al.  Lipid particle composition of the yeast Yarrowia lipolytica depends on the carbon source , 2006, Proteomics.

[7]  Hideo Tanaka,et al.  Application of Maxblend Fermentor® for microbial processes , 1997 .

[8]  S. Papanikolaou,et al.  Repression of reserve lipid turnover in Cunninghamella echinulata and Mortierella isabellina cultivated in multiple‐limited media , 2004, Journal of applied microbiology.

[9]  S. Papanikolaou,et al.  Kinetic profile of the cellular lipid composition in an oleaginous Yarrowia lipolytica capable of producing a cocoa-butter substitute from industrial fats , 2001, Antonie van Leeuwenhoek.

[10]  F. Mantzouridou,et al.  Performance of crude olive pomace oil and soybean oil during carotenoid production by Blakeslea trispora in submerged fermentation. , 2006, Journal of agricultural and food chemistry.

[11]  C. Ratledge.  Yeasts, moulds, algae and bacteria as sources of lipids , 1994 .

[12]  O. Kalantzi,et al.  Growth conditions of Aspergillus sp. ATHUM-3482 for polygalacturonase production , 1997, Applied Microbiology and Biotechnology.

[13]  D. Kekos,et al.  Production and Characterization of Amylase from Calvatia gigantea , 1983, Applied and environmental microbiology.

[14]  J. Nicaud,et al.  Acyl-CoA oxidase, a key step for lipid accumulation in the yeast Yarrowia lipolytica , 2004 .

[15]  Hung-Chang Chen,et al.  Production of γ‐Linolenic Acid by the Fungus Cunninghamella echinulata CCRC 31840 , 1996 .

[16]  H. Jang,et al.  Effect of culture media and conditions on polyunsaturated fatty acids production by Mortierella alpina. , 2005, Bioresource technology.

[17]  A. Steinbüchel,et al.  Accumulation and mobilization of storage lipids by Rhodococcus opacus PD630 and Rhodococcus ruber NCIMB 40126 , 2000, Applied Microbiology and Biotechnology.

[18]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[19]  Sudheer Kumar Singh,et al.  Effects of various process parameters on the production of gamma-linolenic acid in submerged fermentation , 2006 .

[20]  M. Galiotou-Panayotou,et al.  Tannin‐resistant α‐amylase from Calvatia gigantea , 1988 .

[21]  M. Čertík,et al.  Effect of cadmium on lipid composition of Aureobasidium pullulans grown with added extracellular polysaccharides , 2005 .

[22]  Bo Liu,et al.  Optimization of Culture Conditions for Lipid Production by Rhodosporidium toruloides , 2006 .

[23]  S. Narine,et al.  Maximizing the production of γ-linolenic acid in Mortierella ramanniana var. ramanniana as a function of pH, temperature and carbon source, nitrogen source, metal ions and oil supplementation , 2005 .

[24]  D. Somashekar,et al.  Effect of culture conditions on lipid and gamma-linolenic acid production by mucoraceous fungi , 2003 .

[25]  Hung-Chang Chen,et al.  Inoculum effects on the production of γ-linolenic acid by the shake culture of Cunninghamella echinulata CCRC 31840 , 1997 .

[26]  M. Zhu,et al.  An inexpensive medium for production of arachidonic acid by Mortierella alpina , 2003, Journal of Industrial Microbiology and Biotechnology.

[27]  N. Aksöz,et al.  Production of Lactase by Trichoderma Sp. , 2004 .

[28]  T. Antczak,et al.  Relationships between lipases and lipids in mycelia of two Mucor strains , 2006 .

[29]  P. Rodis,et al.  Enhanced polygalacturonase production by Aspergillus niger NRRL‐364 grown on supplemented citrus pectin , 1993 .

[30]  C. Ratledge,et al.  The role of malic enzyme in the regulation of lipid accumulation in filamentous fungi. , 1999, Microbiology.

[31]  M. Dostálek,et al.  Effect of culture conditions on mycelial growth and production of γ-linolenic acid by the fungus Mortierella ramanniana , 1988, Applied Microbiology and Biotechnology.

[32]  Fengwu Bai,et al.  High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture , 2007 .

[33]  Seraphim Papanikolaou,et al.  Single cell oil (SCO) production by Mortierella isabellina grown on high-sugar content media. , 2004, Bioresource technology.

[34]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[35]  S. Papanikolaou,et al.  Modeling Lipid Accumulation and Degradation in Yarrowia lipolytica Cultivated on Industrial Fats , 2003, Current Microbiology.