Molecular Characterization of Anaplasma, Ehrlichia, Babesia, and Borrelia in Ticks from Guangdong Province of Southern China

Tick-borne pathogens have become emerging global health threats in humans and animals. The objective of the present study was to investigate Anaplasma, Ehrlichia, Babesia, and Borrelia in ticks in southern China. Ticks were collected from animals and identified to the species level. Tick-borne Anaplasma, Ehrlichia, Babesia, and Borrelia were tested by PCR assays, and analyzed by sequencing and phylogenetics. We collected 747 ticks, including Rhipicephalus microplus (n=225) and R. sanguineus (n=522), from pet dogs in Guangdong Province of southern China. Using PCR and phylogenetic analysis we showed that these ticks carried Anaplasma platys, Ehrlichia canis, Borrelia miyamotoi, Babesia vogeli, and an unclassified Ehrlichia species in Rhipicephalus ticks. Of these pathogens, B. miyamotoi was first found in southern China. A. platys, E. canis, and B. miyamotoi were zoonotic pathogens that infected both R. microplus and R. sanguineus, whereas Ba. vogeli only infected R. sanguineus, with a prevalence of 0.3%-1.5%. The results of this study showed diversity and complexity of tick-borne pathogens in Guangdong Province of southern China, implying the importance of tick-borne pathogen surveillance and control.

[1]  B. Gessner,et al.  Systematic Review and Meta-analysis of Lyme Disease Data and Seropositivity for Borrelia burgdorferi, China, 2005‒2020 , 2022, Emerging infectious diseases.

[2]  QUAN LIU,et al.  Virome of Rhipicephalus ticks by metagenomic analysis in Guangdong, southern China , 2022, Frontiers in Microbiology.

[3]  P. Díaz,et al.  Diversity of Anaplasma species and importance of mixed infections in roe deer from Spain. , 2021, Transboundary and emerging diseases.

[4]  Gaowa,et al.  Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China , 2021, Parasites & vectors.

[5]  W. Wang,et al.  Identification of a new orthonairovirus associated with human febrile illness in China , 2021, Nature Medicine.

[6]  W. Liu,et al.  Mapping ticks and tick-borne pathogens in China , 2021, Nature Communications.

[7]  Xiaolong Lv,et al.  First detection of Borrelia miyamotoi infections in ticks and humans from the northeast of Inner Mongolia, China. , 2021, Acta tropica.

[8]  Meihua Yang,et al.  Human Tacheng Tick Virus 2 Infection, China, 2019 , 2021, Emerging infectious diseases.

[9]  W. Wang,et al.  A new nairo-like virus associated with human febrile illness in China , 2021, Emerging microbes & infections.

[10]  Xiaoxing Wang,et al.  Molecular evidence of piroplasm infection in companion animals in Hunan Province, China , 2020, BMC Veterinary Research.

[11]  Jia-Fu Jiang,et al.  Anaplasma platys-Like Infection in Goats, Beijing, China. , 2020, Vector borne and zoonotic diseases.

[12]  N. Jin,et al.  A Tentative Tamdy Orthonairovirus Related to Febrile Illness in Northwestern China. , 2020, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[13]  Xuelian Zhang,et al.  Prevalence and molecular characterization of Babesia in pet dogs in Shenzhen, China. , 2020, Comparative immunology, microbiology and infectious diseases.

[14]  L. Kramer,et al.  Emerging Tick-Borne Diseases , 2020, Clinical Microbiology Reviews.

[15]  Xuelian Zhang,et al.  Molecular evidence of Babesia in pet cats in mainland China , 2019, BMC Veterinary Research.

[16]  Quan Liu,et al.  A New Segmented Virus Associated with Human Febrile Illness in China. , 2019, The New England journal of medicine.

[17]  Quan Liu,et al.  Prevalence and Identification of Borrelia burgdorferi Sensu Lato Genospecies in Ticks from Northeastern China. , 2019, Vector borne and zoonotic diseases.

[18]  B. He,et al.  Genomes and seroprevalence of severe fever with thrombocytopenia syndrome virus and Nairobi sheep disease virus in Haemaphysalis longicornis ticks and goats in Hubei, China , 2019, Virology.

[19]  Xiaofeng Zheng,et al.  First description of the pathogenicity of Babesia vogeli in experimentally infected dogs. , 2018, Veterinary parasitology.

[20]  M. Aktas,et al.  Molecular evidence for trans‐stadial transmission of Anaplasma platys by Rhipicephalus sanguineus sensu lato under field conditions , 2018, Medical and veterinary entomology.

[21]  W. Liu,et al.  Borrelia miyamotoi Infections in Humans and Ticks, Northeastern China , 2018, Emerging infectious diseases.

[22]  M. Aktas,et al.  Molecular Evidence for Transstadial Transmission of Ehrlichia canis by Rhipicephalus sanguineus sensu lato Under Field Conditions , 2017, Journal of Medical Entomology.

[23]  K. Olival,et al.  Global hotspots and correlates of emerging zoonotic diseases , 2017, Nature Communications.

[24]  Jian-xun Luo,et al.  Molecular evidence of tick-borne pathogens in Hyalomma anatolicum ticks infesting cattle in Xinjiang Uygur Autonomous Region, Northwestern China , 2017, Experimental and Applied Acarology.

[25]  N. Ismail,et al.  Tick-Borne Emerging Infections: Ehrlichiosis and Anaplasmosis. , 2017, Clinics in laboratory medicine.

[26]  Quan Liu,et al.  Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China , 2016, Frontiers in microbiology.

[27]  X. Xuan,et al.  First molecular detection of tick-borne pathogens in dogs from Jiangxi, China , 2016, The Journal of veterinary medical science.

[28]  L. Fang,et al.  Emerging tick-borne infections in mainland China: an increasing public health threat , 2015, The Lancet Infectious Diseases.

[29]  B. He,et al.  Nairobi Sheep Disease Virus RNA in Ixodid Ticks, China, 2013 , 2015, Emerging infectious diseases.

[30]  E. Breitschwerdt,et al.  Intravascular persistence of Anaplasma platys, Ehrlichia chaffeensis, and Ehrlichia ewingii DNA in the blood of a dog and two family members , 2014, Parasites & Vectors.

[31]  F. Jongejan,et al.  Transmission of Ehrlichia canis by Rhipicephalus sanguineus ticks feeding on dogs and on artificial membranes. , 2013, Veterinary parasitology.

[32]  M. Pittau,et al.  Molecular Investigation and Phylogeny of Anaplasma spp. in Mediterranean Ruminants Reveal the Presence of Neutrophil-Tropic Strains Closely Related to A. platys , 2013, Applied and Environmental Microbiology.

[33]  D. Raoult,et al.  Survey of Anaplasmataceae bacteria in sheep from Senegal , 2013, Tropical Animal Health and Production.

[34]  D. Morrison,et al.  Babesia: a world emerging. , 2012, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[35]  R. Straubinger,et al.  First case of Anaplasma platys infection in a dog from Croatia , 2012, Parasites & Vectors.

[36]  Weizhong Yang,et al.  Fever with thrombocytopenia associated with a novel bunyavirus in China. , 2011, The New England journal of medicine.

[37]  C. L. Fritz.  Emerging tick-borne diseases. , 2009, The Veterinary clinics of North America. Small animal practice.

[38]  S. Haddad,et al.  Clinical, serological, and molecular evidence of ehrlichiosis and anaplasmosis in dogs in Tunisia , 2009, Parasitology Research.

[39]  N. Okabe,et al.  Molecular Survey of Babesia microti, Ehrlichia Species and Candidatus Neoehrlichia mikurensis in Wild Rodents from Shimane Prefecture, Japan , 2007, Microbiology and immunology.

[40]  Y. Rikihisa,et al.  Human Infection with Ehrlichia Canis Accompanied by Clinical Signs in Venezuela , 2006, Annals of the New York Academy of Sciences.

[41]  Á. Sainz,et al.  Genetic characterization of Anaplasma (Ehrlichia) platys in dogs in Spain. , 2006, Journal of veterinary medicine. B, Infectious diseases and veterinary public health.

[42]  James E. Childs,et al.  Ehrlichia chaffeensis: a Prototypical Emerging Pathogen , 2003, Clinical Microbiology Reviews.

[43]  Y. Takahashi,et al.  Genetic and phenotypic analysis of Borrelia miyamotoi sp. nov., isolated from the ixodid tick Ixodes persulcatus, the vector for Lyme disease in Japan. , 1995, International journal of systematic bacteriology.

[44]  F. Veronesi,et al.  Natural infection of Anaplasma platys in dogs from Umbria region (Central Italy). , 2014, Veterinaria italiana.

[45]  H. Sager,et al.  Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland. , 2006, Annals of agricultural and environmental medicine : AAEM.

[46]  Hao Li,et al.  Molecular detection and identi fi cation of relapsing fever Borrelia in ticks and wild small mammals in China , 2022 .