Study on the Experiments of KCNJ11 E23K Variant and Genetic Susceptibility in Type 2 Diabetes Mellitus

Objective To investigate the impact of KCNJ11 gene-combined single nucleotide polymorphism(SNP) variation on type 2 diabetes(T2DM).Method Genomic DNA were extracted from the hemocyte of 100 Chinese residents with T2DM in Anhui.Polymerase chain reactions were used to amplify E23K in the KCNJ11 gene.Then the products were analyzed with electrophoresis.Different outcomes can be observed between normal and T2DM groups by ultraviolet rays.Results The frequency of K allele of the KCNJ11 in type 2 diabetes patients was significantly increased as compared with that in the control subjects(73.5% vs 6.7%,P0.01),also was the geno-typic frequency for KK(72% vs 2%,P0.01).The frequency of K allele and KK genotype showed significant differ-ence between these two groups(P0.01).Conclusion KCNJ11 gene combined KK may directly or indirectly con-tribute to the genetic susceptility of type 2 diabetes.