A method for generating single crystals that rely on internal fluid dynamics of microdroplets.

The single crystallization method by focusing on the characteristic internal fluid dynamics of the microdroplets was explored. Also the theoretical background was discussed, and the droplet size for obtaining only a single crystal within a microdroplet was estimated.

[1]  Kanaka Hettiarachchi,et al.  Controlled microfluidic encapsulation of cells, proteins, and microbeads in lipid vesicles. , 2006, Journal of the American Chemical Society.

[2]  A. Faleiros,et al.  Kinetics of phase change , 2000 .

[3]  S. Chung,et al.  Containerless protein crystal growth in rotating levitated drops , 1998 .

[4]  A. McPherson,et al.  Protein and virus crystal growth on international microgravity laboratory-2. , 1995, Biophysical journal.

[5]  Liang Li,et al.  Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins , 2006, Proceedings of the National Academy of Sciences.

[6]  Toshiro Higuchi,et al.  Droplet formation in a microchannel network. , 2002, Lab on a chip.

[7]  Sophie Pautot,et al.  Engineering asymmetric vesicles , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[8]  Kenichi Yoshikawa,et al.  Spontaneous transfer of phospholipid-coated oil-in-oil and water-in-oil micro-droplets through an oil/water interface. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[9]  Daeyeon Lee,et al.  Double emulsion templated monodisperse phospholipid vesicles. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[10]  A. McPherson Virus and protein crystal growth on earth and in microgravity , 1993 .

[11]  B. Lorber,et al.  Nucleation and growth of thaumatin crystals within a gel under microgravity on STS-95 mission vs. under Earth's gravity , 2001 .

[12]  W. Littke,et al.  Materials: protein single crystal growth under microgravity. , 1984, Science.

[13]  Joseph E. Reiner,et al.  Preparation of nanoparticles by continuous-flow microfluidics , 2008 .

[14]  N. Van Rooijen,et al.  Effect of liposome size on the circulation time and intraorgan distribution of amphipathic poly(ethylene glycol)-containing liposomes. , 1994, Biochimica et biophysica acta.

[15]  S Kim,et al.  Predicting protein diffusion coefficients. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[16]  S. Veesler,et al.  Photochemically induced nucleation in supersaturated and undersaturated thaumatin solutions , 2007 .

[17]  A. deMello,et al.  Droplet microfluidics: recent developments and future applications. , 2011, Chemical communications.

[18]  Hiroyuki Nakamura,et al.  Analysis of Kinetic Behavior of Protein Crystallization in Nanodroplets , 2011 .

[19]  H. Yamaguchi,et al.  X-ray diffraction of protein crystal grown in a nano-liter scale droplet in a microchannel and evaluation of its applicability. , 2012, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[20]  Dimo Kashchiev,et al.  Crystallization and Critical Supercooling of Disperse Liquids , 1994 .

[21]  B. Dardzinski,et al.  Temperature Dependent Change of Apparent Diffusion Coefficient of Water in Normal and Ischemic Brain of Rats , 1994, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[22]  Philippe Laval,et al.  A microfluidic device for investigating crystal nucleation kinetics , 2007 .

[23]  A. McPherson,et al.  Atomic Force Microscopy Studies of Surface Morphology and Growth Kinetics in Thaumatin Crystallization , 1996 .

[24]  S. Quake,et al.  A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[25]  A. D. Clark,et al.  Recent results and new hardware developments for protein crystal growth in microgravity , 1994 .

[26]  B. Sidell,et al.  Temperature affects the diffusion of small molecules through cytosol of fish muscle. , 1987, The Journal of experimental biology.

[27]  Stéphane Veesler,et al.  Reaching One Single and Stable Critical Cluster through Finite-Sized Systems , 2009 .

[28]  A. Hosoi,et al.  Marangoni convection in droplets on superhydrophobic surfaces , 2009, Journal of Fluid Mechanics.

[29]  N. Candoni,et al.  Nucleation and polymorphism explored via an easy-to-use microfluidic tool , 2012 .

[30]  Rustem F Ismagilov,et al.  Formation of Arrayed Droplets by Soft Lithography and Two‐Phase Fluid Flow, and Application in Protein Crystallization , 2004, Advanced materials.

[31]  D. Verdoes,et al.  Induction time and metastability limit in new phase formation , 1991 .

[32]  Rustem F Ismagilov,et al.  A droplet-based, composite PDMS/glass capillary microfluidic system for evaluating protein crystallization conditions by microbatch and vapor-diffusion methods with on-chip X-ray diffraction. , 2004, Angewandte Chemie.

[33]  Pinhas Ben-Tzvi,et al.  Microdroplet generation in gaseous and liquid environments , 2009 .

[34]  P. Vekilov,et al.  Nucleation and Crystallization of Globular Proteins: What we Know and What is Missing , 1996 .

[35]  V. Aswal,et al.  Time evolution of crystallization phase of lysozyme protein in aqueous salt solution as studied by scattering techniques , 2007 .

[36]  Temperature dependence of the diffusion coefficient of nanoparticles , 2008 .

[37]  Solubility of Thaumatin , 2008 .

[38]  R. Ismagilov,et al.  Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets. , 2003, Journal of the American Chemical Society.