Analysis of poly(amidoamine)‐succinamic acid dendrimers by slab‐gel electrophoresis and capillary zone electrophoresis

Ethylenediamine (EDA)‐core poly(amidoamine) (PAMAM) succinamic acid dendrimers (Ex.SAH, where x refers to the generation) were synthesized and analyzed by polyacrylamide gel electrophoresis (PAGE), size‐exclusion chromatography (SEC), potentiometric acid‐base titration, and capillary zone electrophoresis (CZE). Various generations (E1.SAH–E7.SAH) PAMAMs and a succinamic acid terminated core‐shell tecto(dendrimer) (E5(E3.SAH)n) were first analyzed by PAGE. PAGE results show that the relative mobilities of generation 2 to generation 7 dendrimers decreased with the increasing number of generations. The molecular mass of a generation 5 core generation 3 shell tecto(dendrimer) (denoted as E5(E3.SAH)n) was determined to be between the Mw of E6.SAH and E7.SAH. CZE analysis allowed the evaluation of electrophoretic properties of given‐generation dendrimers. The electrophoretic mobilities of individual generations PAMAM polyanions are similar, indicating that the separation mainly depends on their approximately identical charge/mass ratio. The E5(E3.SAH)n tectodendrimer had a lower electrophoretic mobility, which was consistent with its lower charge/mass ratio. The combination of PAGE and CZE analysis provides an alternative and effective way to characterize this group of PAMAM‐succinamic acid dendrimers.

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