论文信息 - Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins - 字舞流文

Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins

Recombinant His-tagged XendoU, a eukaryotic endoribonuclease, appeared to aggregate in the presence of divalent cations. Monodisperse protein which yielded crystals diffracting to 2.2 Å was obtained by addition of EDTA.

Beatrice Vallone | M. Brunori | B. Vallone | I. Bozzoni | F. Renzi | Pietro Laneve | E. Caffarelli | Irene Bozzoni | Maurizio Brunori | Fabiana Renzi | Gianna Panetta | Massimo Arceci | Pietro Laneve | Elisa Caffarelli | G. Panetta | Massimo Arceci

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