Growth inhibition of prostate cell lines in vitro by phyto-oestrogens.

OBJECTIVE To assess a range of phyto-oestrogens as moderators of growth and metabolism in several prostate cell lines. MATERIALS AND METHODS Four prostate cell lines (PNT-1/A, PNT-2, PC-3 and DU145) were challenged with different doses of five phyto-oestrogens (biochanin A, daidzein, genistein, genistin and nordihydroguaiaretic acid) over 3 days in culture. Cell proliferation was assessed by incorporation of 5-bromo-2'-deoxyuridine (BrdU) and metabolic activity by cleavage of a tetrazolium salt (XTT). RESULTS Growth and metabolism were inhibited with all compounds and cell lines (e.g. the dose for 50% inhibition of proliferation of PC-3 cells by genistein was 38 micromol/L); differences in the patterns of results suggested that different mechanisms operated, but there was no evidence for any synergistic activity on the inhibition of cell proliferation. CONCLUSION These results offer further support for the hypothesized role of phyto-oestrogens as dietary protectors against prostatic cancer.

[1]  R. Ward.  Lignans, neolignans, and related compounds. , 1995, Natural product reports.

[2]  John D. Potter,et al.  Food, nutrition and the prevention of cancer : a global perspective , 2001 .

[3]  W. Fair,et al.  Cancer of the prostate: a nutritional disease? , 1997, Urology.

[4]  S. Barnes,et al.  Phyto-oestrogens and breast cancer , 1997, The Lancet.

[5]  F. Stephens,et al.  Phytoestrogens and prostate cancer: possible preventive role , 1997, The Medical journal of Australia.

[6]  J. Gustafsson.  Estrogen receptor β — Getting in on the action? , 1997, Nature Medicine.

[7]  J. Gustafsson,et al.  Cloning of a novel receptor expressed in rat prostate and ovary. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[8]  K. Griffiths,et al.  The preventive role of diet in prostatic disease. , 1996, British journal of urology.

[9]  K. Griffiths,et al.  Inhibition of 5 alpha-reductase in genital skin fibroblasts and prostate tissue by dietary lignans and isoflavonoids. , 1995, The Journal of endocrinology.

[10]  H. Adlercreutz,et al.  Genistein, a dietary ingested isoflavonoid, inhibits cell proliferation and in vitro angiogenesis. , 1995, The Journal of nutrition.

[11]  O. Cussenot,et al.  Functional expression of sv40 in normal human prostatic epithelial and fibroblastic cells - differentiation pattern of nontumorigenic cell-lines. , 1995, International journal of oncology.

[12]  H. Adlercreutz,et al.  Genistein is an effective stimulator of sex hormone-binding globulin production in hepatocarcinoma human liver cancer cells and suppresses proliferation of these cells in culture , 1993, Steroids.

[13]  H. Adlercreutz,et al.  Genistein, a dietary-derived inhibitor of in vitro angiogenesis. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[14]  H. Adlercreutz,et al.  Inhibition of human aromatase by mammalian lignans and isoflavonoid phytoestrogens , 1993, The Journal of Steroid Biochemistry and Molecular Biology.

[15]  H. Adlercreutz.  Diet, Breast Cancer, and Sex Hormone Metabolism , 1990, Annals of the New York Academy of Sciences.

[16]  W. L. Beeson,et al.  Cohort study of diet, lifestyle, and prostate cancer in adventist men , 1989, Cancer.

[17]  M. Shibuya,et al.  Genistein, a specific inhibitor of tyrosine-specific protein kinases. , 1987, The Journal of biological chemistry.

[18]  W. McGuire,et al.  Phytoestrogen interaction with estrogen receptors in human breast cancer cells. , 1978, Endocrinology.

[19]  S. Barnes,et al.  Genistein and biochanin A inhibit the growth of human prostate cancer cells but not epidermal growth factor receptor tyrosine autophosphorylation , 1993, The Prostate.

[20]  H. Adlercreutz,et al.  Effect of dietary components, including lignans and phytoestrogens, on enterohepatic circulation and liver metabolism of estrogens and on sex hormone binding globulin (SHBG). , 1987, Journal of steroid biochemistry.