In order to provide suitable blood transfusion samples to patients, the blood bag should be kept at a uniformly maintained temperature to prevent deterioration during transportation. Therefore, this paper presents a blood monitoring and management system for use in hospitals. This system may continuously report the temperature of the blood bank refrigerator, track the location of a blood bag to increase staff operation efficiency, and can confirm that the assigned blood bag was transported to the intended patient in need of transfusion. We developed and demonstrated the clinical usability of the combined blood temperature management and tracking system using a ubiquitous sensor network and RFID (Radio Frequency Identification) technology.