Improved fertilization and implantation rates after non-touch zona pellucida microdrilling of mouse oocytes with a 1.48 microm diode laser beam.

The safety of microdrilling the zona pellucida of mouse oocytes with a 1.48 microm diode laser has been investigated by determining the ability of mouse oocytes to fertilize in vitro and develop in vivo. Mice born after transfer of control and zona pellucida-microdrilled embryos into foster mothers were submitted to anatomical and immunohistochemical investigations, and their aptitude to breed was assessed in two subsequent generations. Decoronization of the oocytes with hyaluronidase induced a reduction of the fertilization and implantation rates, which was attributed to a zona hardening phenomenon. After laser zona pellucida microdrilling, these rates were restored to those obtained with embryos derived from untreated oocyte-cumulus complexes. Pups derived from zona pellucida microdrilled embryos were comparable with those obtained from control embryos, confirming the lack of deleterious effects of the laser treatment. In conclusion, the 1.48 microm diode laser allows safe microdrilling of the zona pellucida of mouse oocytes after decoronization with hyaluronidase. Based on the health of the F2 generation and the lack of neuroanatomical and neurochemical differences, we concluded that this technology may be investigated in the human, particularly when the zona pellucida represents the main impediment for fertilization or embryo hatching.

[1]  Peter Fuhrberg,et al.  Photoablation of oocyte zona pellucida by erbium-yag laser for in-vitro fertilisation in severe male infertility , 1992, The Lancet.

[2]  J. Lauder,et al.  Neurotransmitters as growth regulatory signals: role of receptors and second messengers , 1993, Trends in Neurosciences.

[3]  S. Bassil,et al.  Micromanipulation of mouse gametes with laser microbeam and optical tweezers. , 1995, Human reproduction.

[4]  K. Mclaughlin,et al.  Comparison of zona cutting and zona drilling as techniques for assisted fertilization in the mouse. , 1988, Journal of reproduction and fertility.

[5]  K. Schütze,et al.  Zona drilling and sperm insertion with combined laser microbeam and optical tweezers. , 1994, Fertility and sterility.

[6]  M. Derde,et al.  Citations from the Literature , 2003 .

[7]  J B Russell,et al.  Laser micromanipulation in the mouse embryo: a novel approach to zona drilling. , 1992, Fertility and sterility.

[8]  T. Maeda,et al.  Visualization of detailed acetylcholinesterase fiber and neuron staining in rat brain by a sensitive histochemical procedure. , 1986, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[9]  H E Malter,et al.  Partial zona dissection of the human oocyte: a nontraumatic method using micromanipulation to assist zona pellucida penetration. , 1989, Fertility and sterility.

[10]  D F Katz,et al.  Biophysical properties of the zona pellucida measured by capillary suction: is zona hardening a mechanical phenomenon? , 1988, The Journal of experimental zoology.

[11]  D. van der Kooy,et al.  The mouse mutation reeler causes increased adhesion within a subpopulation of early postmitotic cortical neurons , 1995, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[12]  A. Lopata,et al.  A zona opening procedure for improving in vitro fertilization at low sperm concentrations: a mouse model. , 1989, Fertility and sterility.

[13]  K Rink,et al.  Microdissection of mouse and human zona pellucida using a 1.48-microns diode laser beam: efficacy and safety of the procedure. , 1995, Fertility and sterility.

[14]  M. Berns,et al.  Zona opening with 308 nm XeCl excimer laser improves fertilization by spermatozoa from long-term vasectomized mice. , 1993, Human reproduction.

[15]  C. Sotelo.  Mutant mice and the formation of cerebellar circuitry , 1980, Trends in Neurosciences.

[16]  J. Gordon.  Use of Micromanipulation for Increasing the Efficiency of Mammalian Fertilization in Vitro a , 1988, Annals of the New York Academy of Sciences.

[17]  D. Palanker,et al.  The efficacy and safety of zona pellucida drilling by a 193-nm excimer laser. , 1993, Fertility and sterility.

[18]  Klaus Rink,et al.  Non‐contact microdrilling of mouse zona pellucida with an objective‐delivered 1.48‐μm diode laser , 1996, Lasers in surgery and medicine.

[19]  J. Gordon,et al.  Restoration of normal implantation rates in mouse embryos with a hatching impairment by use of a new method of assisted hatching. , 1993, Fertility and sterility.

[20]  D. Whittingham.  Culture of mouse ova. , 1971, Journal of reproduction and fertility. Supplement.

[21]  H Malter,et al.  Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. , 1990, Human reproduction.

[22]  X. Zhang,et al.  Studies on zona hardening in rat oocytes that are matured in vitro in a serum‐free medium , 1991, Molecular reproduction and development.

[23]  K. Wigglesworth,et al.  Comparison of embryonic developmental competence of mouse oocytes grown with and without serum , 1992, Molecular reproduction and development.

[24]  C. Versaci,et al.  Andrology: Seventeen live births after the use of an erbium-yytrium aluminium garnet laser in the treatment of male factor infertility , 1994 .

[25]  R. Palmiter,et al.  Targeted disruption of the tyrosine hydroxylase gene reveals that catecholamines are required for mouse fetal development , 1995, Nature.

[26]  J W Gordon,et al.  Assisted fertilization by zona drilling: a mouse model for correction of oligospermia. , 1986, The Journal of experimental zoology.

[27]  C. Matthews,et al.  Freeze-thaw-induced changes of the zona pellucida explains decreased rates of fertilization in frozen-thawed mouse oocytes. , 1990, Journal of reproduction and fertility.

[28]  Guy P. Delacretaz,et al.  1.48-um diode laser microdissection of the zona pellucida of mouse zygotes , 1994, SPIE LASE.

[29]  J W Gordon,et al.  Clinical evaluation of three approaches to micromanipulation-assisted fertilization. , 1990, Fertility and sterility.

[30]  P. Martínez,et al.  Effect of anti-human sperm monoclonal antibodies on mouse in vitro fertilization. , 1994, Immunological investigations.

[31]  M. Oermann,et al.  [Clinical evaluation]. , 1981, Ugeskrift for laeger.

[32]  A. Handyside,et al.  Embryo biopsy strategies for preimplantation diagnosis. , 1993, Fertility and sterility.

[33]  W Feichtinger,et al.  Use of lasers in assisted fertilization and hatching. , 1994, Human reproduction.

[34]  C. Peterson,et al.  Role of the cumulus in the selection of morphologically normal sperm and induction of the acrosome reaction during human in vitro fertilization. , 1993, Archives of andrology.

[35]  N. Laufer,et al.  Fertilization of human oocytes by sperm from infertile males after zona pellucida drilling. , 1988, Fertility and sterility.