Advances in tracking hematopoiesis at the single-cell level

Purpose of reviewStudying heterogeneous populations, such as hematopoietic stem cells (HSCs), requires continuous long-term observation of living cells at the single-cell level. The purpose of this review is to discuss recent advances in technologies required for continuous single-cell analysis and the contribution of this approach to find answers in hematopoiesis research. Recent findingsContinuous long-term imaging at the single-cell level still requires custom-made hardware, software and manual in-depth analysis of large amounts of data. Despite these technical difficulties, continuous time-lapse imaging and single-cell tracking are increasingly used in hematopoiesis research. It has already contributed to answering decades-old questions. SummaryContinuous long-term single-cell analysis is indispensable for a comprehensive analysis of dynamic processes in heterogeneous cell populations. Despite many remaining technological hurdles, this approach is increasingly used in hematopoiesis research.

[1]  G. Sauvageau,et al.  Asymmetric segregation and self-renewal of hematopoietic stem and progenitor cells with endocytic Ap2a2. , 2012, Blood.

[2]  Ingo Roeder,et al.  On the symmetry of siblings: automated single-cell tracking to quantify the behavior of hematopoietic stem cells in a biomimetic setup. , 2012, Experimental hematology.

[3]  M. R. Costa,et al.  Using an adherent cell culture of the mouse subependymal zone to study the behavior of adult neural stem cells on a single-cell level , 2011, Nature Protocols.

[4]  D. Rapoport,et al.  A Novel Validation Algorithm Allows for Automated Cell Tracking and the Extraction of Biologically Meaningful Parameters , 2011, PloS one.

[5]  D. Kent,et al.  Ontogeny stage-independent and high-level clonal expansion in vitro of mouse hematopoietic stem cells stimulated by an engineered NUP98-HOX fusion transcription factor. , 2011, Blood.

[6]  D. Kent,et al.  High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays , 2011, Nature Methods.

[7]  Timm Schroeder,et al.  Long-term single-cell imaging of mammalian stem cells , 2011, Nature Methods.

[8]  Felipe Ortega,et al.  Continuous live imaging of adult neural stem cell division and lineage progression in vitro , 2011, Development.

[9]  V. Ntziachristos.  Going deeper than microscopy: the optical imaging frontier in biology , 2010, Nature Methods.

[10]  S. V. Anisimov,et al.  Risks and Mechanisms of Oncological Disease Following Stem Cell Transplantation , 2010, Stem Cell Reviews and Reports.

[11]  Timm Schroeder,et al.  Hematopoietic stem cell heterogeneity: subtypes, not unpredictable behavior. , 2010, Cell stem cell.

[12]  K. Kissa,et al.  Blood stem cells emerge from aortic endothelium by a novel type of cell transition , 2010, Nature.

[13]  Timm Schroeder,et al.  The electronic crystal ball: predicting cell fate from time-lapse data , 2010, Nature Methods.

[14]  Andrew R. Cohen,et al.  Computational prediction of neural progenitor cell fates , 2010, Nature Methods.

[15]  S. Janmohamed,et al.  Intermediate-term hematopoietic stem cells with extended but time-limited reconstitution potential. , 2010, Cell stem cell.

[16]  D. Stainier,et al.  Hematopoietic stem cells derive directly from aortic endothelium during development , 2009, Nature.

[17]  Philipp S. Hoppe,et al.  The transcription factors STAT5A/B regulate GM-CSF-mediated granulopoiesis. , 2009, Blood.

[18]  T. Cheng,et al.  Stem Cell Traits in Long-term Co-culture Revealed by Time-lapse Imaging , 2009, Leukemia.

[19]  Philipp S. Hoppe,et al.  Hematopoietic Cytokines Can Instruct Lineage Choice , 2009, Science.

[20]  Andreas Trumpp,et al.  Hematopoietic Stem Cells Reversibly Switch from Dormancy to Self-Renewal during Homeostasis and Repair , 2008, Cell.

[21]  Shin-Ichi Nishikawa,et al.  Continuous single-cell imaging of blood generation from haemogenic endothelium , 2009, Nature.

[22]  P. Lio’,et al.  Hematopoietic Stem Cells Reversibly Switch from Dormancy to Self-Renewal during Homeostasis and Repair , 2008, Cell.

[23]  Liran Carmel,et al.  Potency and fate specification in CNS stem cell populations in vitro. , 2008, Cell stem cell.

[24]  I. Weissman,et al.  The origins of the identification and isolation of hematopoietic stem cells, and their capability to induce donor-specific transplantation tolerance and treat autoimmune diseases. , 2008, Blood.

[25]  D. Kent,et al.  Steel factor coordinately regulates the molecular signature and biologic function of hematopoietic stem cells. , 2008, Blood.

[26]  Timm Schroeder,et al.  Imaging stem-cell-driven regeneration in mammals , 2008, Nature.

[27]  L. Zon,et al.  Hematopoiesis: An Evolving Paradigm for Stem Cell Biology , 2008, Cell.

[28]  Ryan Brinkman,et al.  Long-term propagation of distinct hematopoietic differentiation programs in vivo. , 2007, Cell stem cell.

[29]  Tony J Collins,et al.  ImageJ for microscopy. , 2007, BioTechniques.

[30]  P. Wernet,et al.  Asymmetric cell division within the human hematopoietic stem and progenitor cell compartment: identification of asymmetrically segregating proteins. , 2007, Blood.

[31]  I. Weissman,et al.  Differential Expression of α2 Integrin Separates Long‐Term and Short‐Term Reconstituting Lin−/loThy1.1loc‐kit+ Sca‐1+ Hematopoietic Stem Cells , 2006 .

[32]  A. Trumpp,et al.  Bone-marrow haematopoietic-stem-cell niches , 2006, Nature Reviews Immunology.

[33]  S. Morrison,et al.  Supplemental Experimental Procedures , 2022 .

[34]  T. Schroeder.  Tracking Hematopoiesis at the Single Cell Level , 2005, Annals of the New York Academy of Sciences.

[35]  Keisuke Ito,et al.  Tie2/Angiopoietin-1 Signaling Regulates Hematopoietic Stem Cell Quiescence in the Bone Marrow Niche , 2004, Cell.

[36]  Haiyang Huang,et al.  Identification of the haematopoietic stem cell niche and control of the niche size , 2003, Nature.

[37]  D. Scadden,et al.  Osteoblastic cells regulate the haematopoietic stem cell niche , 2003, Nature.

[38]  H. Sieburg,et al.  Deterministic regulation of hematopoietic stem cell self-renewal and differentiation. , 2002, Blood.

[39]  Hiromitsu Nakauchi,et al.  Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell , 1996, Science.

[40]  A. S. Conner,et al.  Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo , 1996, The Journal of experimental medicine.

[41]  I. Weissman,et al.  Searching for hematopoietic stem cells: evidence that Thy-1.1lo Lin- Sca-1+ cells are the only stem cells in C57BL/Ka-Thy-1.1 bone marrow , 1992, The Journal of experimental medicine.

[42]  I. Weissman,et al.  Purification and characterization of mouse hematopoietic stem cells. , 1988, Science.

[43]  A. I.,et al.  Neural Field Continuum Limits and the Structure–Function Partitioning of Cognitive–Emotional Brain Networks , 2023, Biology.

[44]  H. Blau,et al.  Perturbation of single hematopoietic stem cell fates in artificial niches. , 2009, Integrative biology : quantitative biosciences from nano to macro.

[45]  David W. Rowe,et al.  Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche , 2009, Nature.

[46]  Anne E Carpenter,et al.  CellProfiler: free, versatile software for automated biological image analysis. , 2007, BioTechniques.

[47]  I. Weissman,et al.  Differential expression of alpha2 integrin separates long-term and short-term reconstituting Lin-/loThy1.1(lo)c-kit+ Sca-1+ hematopoietic stem cells. , 2006, Stem cells.

[48]  高野 弥奈.  Asymmetric division and lineage commitment at the level of hematopoietic stem cells : inference from differentiation in daughter cell and granddaughter cell pairs , 2004 .