Directly coaxial 3D bioprinting of large-scale vascularized tissue constructs

Three-dimensional (3D) bioprinting of soft large-scale tissues in vitro is still a big challenge due to two limitations, (i) the lack of an effective way to print fine nutrient delivery channels (NDCs) inside the cell-laden structures above the millimetre level; (ii) the need for a feasible strategy to vascularize NDCs. Here, a novel 3D bioprinting method is reported to directly print cell-laden structures with effectively vascularized NDCs. Bioinks with desired tissue cells and endothelial cells (ECs) are separately and simultaneously printed from the outside (mixed with GelMA) and inside (mixed with gelatin) of a coaxial nozzle. As a result, the printed large-scale tissue consists of sheath-core fibers. At the same time, when the core fibers are dissolved to generate channels, the ECs deposit and adhere to the channels automatically. With this method, 3D cell-laden, vascularized tissue constructs (⩾1 cm) with a long-term culture (⩾20 d) are firstly reported. Specifically, vascularized cancer tissue constructs and osteogenic tissue constructs were generated. Considering the above advantages, this advanced bioprinting strategy has significant potential for building large-scale vascularized tissue constructs for applications in tissue engineering, and possibly even in regenerative medicine and organ repair.

[1]  Jianzhong Fu,et al.  Sacrificial microgel-laden bioink-enabled 3D bioprinting of mesoscale pore networks , 2020 .

[2]  Hui Lin,et al.  Why choose 3D bioprinting? Part II: methods and bioprinters , 2020, Bio-Design and Manufacturing.

[3]  Yong He,et al.  Synchronous 3D Bioprinting of Large‐Scale Cell‐Laden Constructs with Nutrient Networks , 2019, Advanced healthcare materials.

[4]  Yong He,et al.  Why choose 3D bioprinting? Part I: a brief introduction of 3D bioprinting for the beginners , 2019, Bio-Design and Manufacturing.

[5]  Lijie Sun,et al.  3D printing of biomimetic vasculature for tissue regeneration , 2019, Materials Horizons.

[6]  Ali Khademhosseini,et al.  Multiscale bioprinting of vascularized models. , 2019, Biomaterials.

[7]  Jianzhong Fu,et al.  Rapid assembling organ prototypes with controllable cell-laden multi-scale sheets , 2019, Bio-Design and Manufacturing.

[8]  D. Cho,et al.  3D Cell Printing of Perfusable Vascularized Human Skin Equivalent Composed of Epidermis, Dermis, and Hypodermis for Better Structural Recapitulation of Native Skin , 2018, Advanced healthcare materials.

[9]  Jianzhong Fu,et al.  Fiber-Based Mini Tissue with Morphology-Controllable GelMA Microfibers. , 2018, Small.

[10]  Gary J Hooper,et al.  Bio-resin for high resolution lithography-based biofabrication of complex cell-laden constructs , 2018, Biofabrication.

[11]  Ali Khademhosseini,et al.  Bioinks for 3D bioprinting: an overview. , 2018, Biomaterials science.

[12]  Jianzhong Fu,et al.  3D Bioprinting of Low-Concentration Cell-Laden Gelatin Methacrylate (GelMA) Bioinks with a Two-Step Cross-linking Strategy. , 2018, ACS applied materials & interfaces.

[13]  Dong-Woo Cho,et al.  Tissue Engineered Bio‐Blood‐Vessels Constructed Using a Tissue‐Specific Bioink and 3D Coaxial Cell Printing Technique: A Novel Therapy for Ischemic Disease , 2017 .

[14]  L. Bertassoni,et al.  A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs , 2017, Scientific Reports.

[15]  Ali Khademhosseini,et al.  Extrusion Bioprinting of Shear‐Thinning Gelatin Methacryloyl Bioinks , 2017, Advanced healthcare materials.

[16]  Wei Zhu,et al.  Direct 3D bioprinting of prevascularized tissue constructs with complex microarchitecture. , 2017, Biomaterials.

[17]  Liliang Ouyang,et al.  A Generalizable Strategy for the 3D Bioprinting of Hydrogels from Nonviscous Photo‐crosslinkable Inks , 2017, Advanced materials.

[18]  Y. S. Zhang,et al.  Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip. , 2016, Biomaterials.

[19]  Ali Khademhosseini,et al.  Direct 3D bioprinting of perfusable vascular constructs using a blend bioink. , 2016, Biomaterials.

[20]  Mark A. Skylar-Scott,et al.  Three-dimensional bioprinting of thick vascularized tissues , 2016, Proceedings of the National Academy of Sciences.

[21]  A. Khademhosseini,et al.  Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. , 2015, Biomaterials.

[22]  Liang Ma,et al.  Coaxial nozzle-assisted 3D bioprinting with built-in microchannels for nutrients delivery. , 2015, Biomaterials.

[23]  Thomas J Ober,et al.  Microfluidic Printheads for Multimaterial 3D Printing of Viscoelastic Inks , 2015, Advanced materials.

[24]  S. Yoo,et al.  Creating perfused functional vascular channels using 3D bio-printing technology. , 2014, Biomaterials.

[25]  A. Khademhosseini,et al.  Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs. , 2014, Lab on a chip.

[26]  J. Lewis,et al.  3D Bioprinting of Vascularized, Heterogeneous Cell‐Laden Tissue Constructs , 2014, Advanced materials.

[27]  John J. Vericella,et al.  High‐Throughput Printing via Microvascular Multinozzle Arrays , 2013, Advanced materials.

[28]  Brendon M. Baker,et al.  Rapid casting of patterned vascular networks for perfusable engineered 3D tissues , 2012, Nature materials.

[29]  Ali Khademhosseini,et al.  Functional Human Vascular Network Generated in Photocrosslinkable Gelatin Methacrylate Hydrogels , 2012, Advanced functional materials.

[30]  J. Lewis,et al.  Omnidirectional Printing of 3D Microvascular Networks , 2011, Advanced materials.

[31]  A. Khademhosseini,et al.  Cell-laden microengineered gelatin methacrylate hydrogels. , 2010, Biomaterials.

[32]  L. Niklason,et al.  Scaffold-free vascular tissue engineering using bioprinting. , 2009, Biomaterials.

[33]  Jeroen Rouwkema,et al.  Vascularization in tissue engineering. , 2008, Trends in biotechnology.

[34]  Dai Fukumura,et al.  Engineering vascularized tissue , 2005, Nature Biotechnology.

[35]  Noo Li Jeon,et al.  Diffusion limits of an in vitro thick prevascularized tissue. , 2005, Tissue engineering.

[36]  J. Lewis,et al.  Chaotic mixing in three-dimensional microvascular networks fabricated by direct-write assembly , 2003, Nature materials.

[37]  P. Carmeliet,et al.  Angiogenesis in cancer and other diseases , 2000, Nature.

[38]  Marcel A. Heinrich,et al.  Rapid Continuous Multimaterial Extrusion Bioprinting , 2017, Advanced materials.

[39]  A. Khademhosseini,et al.  Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs Using Low‐Viscosity Bioink , 2016, Advanced materials.

[40]  Brendon M. Baker,et al.  Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues , 2012 .

[41]  Yasuaki Seki,et al.  Biological materials: Structure and mechanical properties , 2008 .

[42]  H. Lodish.  Molecular Cell Biology , 1986 .