Evaluation of weaning performance of California halibut (Paralichthys californicus) larvae using growth, survival and digestive proteolytic activity

This study evaluated weaning success of California halibut, Paralichthys californicus, larvae onto a microdiet at various stages of development utilizing growth, survival and digestive enzyme activity. Weaning onto a microdiet was evaluated at 16, 26, 36 and 46 days posthatch (dph). Alkaline and acid proteases and leucine aminopeptidase activities were measured after weaning and compared between the weaned treatment and Artemia-fed controls. Survival was significantly lower in the microdiet-fed treatments compared to the control groups. Growth was significantly reduced in all weaning treatments compared to the control, except for the 46 dph group. No differences in enzyme activities were detected between treatment and diet at 16 and 26 dph; however, activities were higher for the microdiet-fed larvae at 36 and 46 dph. This study demonstrates that California halibut larvae possess a differentiated and effective digestive system early in development and can be weaned with relative success (>40% survival) before completion of the metamorphosis (i.e., 36 dph). The lack of weaning success at an early date cannot be entirely because of the absence of a functional stomach but could be related to, among other factors, the low-microdiet ingestion rates observed and higher leaching of smaller microdiets.

[1]  J. Lazo,et al.  A method to determine protein digestibility of microdiets for larval and early juvenile fish , 2009 .

[2]  S. Morais,et al.  Digestive physiology of marine fish larvae: Hormonal control and processing capacity for proteins, peptides and amino acids , 2007 .

[3]  C. Cahu,et al.  Dietary modulation of some digestive enzymes and Metabolic processes in developing marine fish: Applications to diet formulation , 2007 .

[4]  C. Arnold,et al.  Characterization of digestive enzymes during larval development of red drum (Sciaenops ocellatus) , 2007 .

[5]  J. Lazo,et al.  Proteolytic Activity in California Halibut Larvae (Paralichthys californicus) , 2006 .

[6]  R. Piedrahita,et al.  Development of Digestive Enzymes in California Halibut Paralichthys californicus Larvae , 2006, Fish Physiology and Biochemistry.

[7]  P. Quazuguel,et al.  Amylase and trypsin responses to intake of dietary carbohydrate and protein depend on the developmental stage in sea bass (Dicentrarchus labrax) larvae , 1996, Fish Physiology and Biochemistry.

[8]  Alam,et al.  Effects of protein and lipid sources on the growth and survival of red sea bream Pagrus major and Japanese flounder Paralichthys olivaceus receiving micro‐bound diets during larval and early juvenile stage , 2004 .

[9]  R. Piedrahita,et al.  Ontogenetic development of the digestive system in California halibut (Paralichthys californicus) with notes on feeding practices , 2004 .

[10]  R. Piedrahita,et al.  Development of California Halibut, Paralichthys californicus, Culture , 2004 .

[11]  M. T. Dinis,et al.  Digestive enzymes profile of Solea senegalensis post larvae fed Artemia and a compound diet , 2002, Fish Physiology and Biochemistry.

[12]  C. Langdon.  Microparticle types for delivering nutrients to marine fish larvae , 2003 .

[13]  C. Cahu,et al.  Effect of dietary phospholipid level and phospholipid:neutral lipid value on the development of sea bass (Dicentrarchus labrax) larvae fed a compound diet , 2003, British Journal of Nutrition.

[14]  C. Audet,et al.  Early weaning of winter flounder (Pseudopleuronectes americanus Walbaum) larvae on a commercial microencapsulated diet , 2003 .

[15]  R. Piedrahita,et al.  Morphological development and allometric growth patterns in hatchery-reared California halibut larvae , 2002 .

[16]  S. Kolkovski.  Digestive enzymes in fish larvae and juveniles—implications and applications to formulated diets , 2001 .

[17]  C. Cahu,et al.  Substitution of live food by formulated diets in marine fish larvae , 2001 .

[18]  M. T. Dinis,et al.  Co-feeding microparticulate diets with algae : Toward eliminating the need of zooplankton at first feeding in larval red drum (Sciaenops ocellatus) , 2000 .

[19]  M. Yúfera,et al.  A highly efficient microencapsulated food for rearing early larvae of marine fish , 1999 .

[20]  M. Planas,et al.  Larviculture of marine fish: problems and perspectives , 1999 .

[21]  D. Bengtson,et al.  Green-Water Rearing and Delayed Weaning Improve Growth and Survival of Summer Flounder , 1999 .

[22]  J. Cañavate,et al.  Influence of co-feeding larvae with live and inert diets on weaning the sole Solea senegalensis onto commercial dry feeds , 1999 .

[23]  H. Daniels,et al.  Weaning Success of Southern Flounder Juveniles: Effect of Changeover Period and Diet Type on Growth and Survival , 1999 .

[24]  G. Rosenlund,et al.  Co-feeding marine fish larvae with inert and live diets , 1997 .

[25]  J. Verreth,et al.  Larval Nutritional Physiology: Studies with Clarias gariepinus, Coregonus lavaretus and Scophthalmus maximus , 1993 .

[26]  M. Vasseur,et al.  Mécanismes moléculaires d'activation et d'inhibition de la sucrase intestinale par les cations alcalins et par les protons , 1984 .

[27]  K. Dąbrowski.  The feeding of fish larvae : present « state of the art » and perspectives , 1984 .

[28]  J. Grendell,et al.  Digestive end products mobilize secretory proteins from subcellular stores in the pancreas. , 1981, The American journal of physiology.

[29]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[30]  W. Garrett,et al.  Re-Evaluation of the Relationship between Carcass Density and Body Composition of Beef Steers , 1969 .