Polypyrimidine tract binding protein blocks the 5' splice site-dependent assembly of U2AF and the prespliceosomal E complex.

[1]  Robert Castelo,et al.  Regulation of Fas alternative splicing by antagonistic effects of TIA-1 and PTB on exon definition. , 2005, Molecular cell.

[2]  F. Clark,et al.  Understanding alternative splicing: towards a cellular code , 2005, Nature Reviews Molecular Cell Biology.

[3]  Douglas L Black,et al.  Exon repression by polypyrimidine tract binding protein. , 2005, RNA.

[4]  D. Black.  A simple answer for a splicing conundrum. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[5]  O. Kent,et al.  Characterization of a U2AF-Independent Commitment Complex (E′) in the Mammalian Spliceosome Assembly Pathway , 2005, Molecular and Cellular Biology.

[6]  C. Shin,et al.  Cell signalling and the control of pre-mRNA splicing , 2004, Nature Reviews Molecular Cell Biology.

[7]  Sharlene R. Lim,et al.  Commitment to splice site pairing coincides with A complex formation. , 2004, Molecular cell.

[8]  Michael R. Green,et al.  U2AF homology motifs: protein recognition in the RRM world. , 2004, Genes & development.

[9]  T. Nilsen.  The spliceosome: the most complex macromolecular machine in the cell? , 2003, BioEssays : news and reviews in molecular, cellular and developmental biology.

[10]  D. Black.  Mechanisms of alternative pre-messenger RNA splicing. , 2003, Annual review of biochemistry.

[11]  Melissa S Jurica,et al.  Pre-mRNA splicing: awash in a sea of proteins. , 2003, Molecular cell.

[12]  R. Reed,et al.  Coupling transcription, splicing and mRNA export. , 2003, Current opinion in cell biology.

[13]  Michael Sattler,et al.  Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP. , 2003, Molecular cell.

[14]  T. Cooper,et al.  Antagonistic regulation of alpha-actinin alternative splicing by CELF proteins and polypyrimidine tract binding protein. , 2003, RNA.

[15]  Douglas L. Black,et al.  Roles for SR Proteins and hnRNP A1 in the Regulation of c-src Exon N1 , 2003, Molecular and Cellular Biology.

[16]  J. Valcárcel,et al.  The splicing regulator TIA‐1 interacts with U1‐C to promote U1 snRNP recruitment to 5′ splice sites , 2002, The EMBO journal.

[17]  M. Wilm,et al.  Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[18]  Henning Urlaub,et al.  Small Nuclear Ribonucleoprotein Remodeling During Catalytic Activation of the Spliceosome , 2002, Science.

[19]  E. Wagner,et al.  RNAi-mediated PTB depletion leads to enhanced exon definition. , 2002, Molecular cell.

[20]  Henning Urlaub,et al.  Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD‐box protein , 2002, The EMBO journal.

[21]  Steven P. Gygi,et al.  Comprehensive proteomic analysis of the human spliceosome , 2002, Nature.

[22]  J. Griffith,et al.  Purification and electron microscopic visualization of functional human spliceosomes , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[23]  O. Kent,et al.  Early organization of pre-mRNA during spliceosome assembly , 2002, Nature Structural Biology.

[24]  Bosiljka Tasic,et al.  Alternative pre-mRNA splicing and proteome expansion in metazoans , 2002, Nature.

[25]  D. Bentley.  The mRNA assembly line: transcription and processing machines in the same factory. , 2002, Current opinion in cell biology.

[26]  Nikolaus Grigorieff,et al.  Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. , 2002, RNA.

[27]  A. Kornblihtt,et al.  Alternative splicing: multiple control mechanisms and involvement in human disease. , 2002, Trends in genetics : TIG.

[28]  T. Cooper,et al.  Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing. , 2002, Molecular cell.

[29]  A. Furger,et al.  Integrating mRNA Processing with Transcription , 2002, Cell.

[30]  Massimo Caputi,et al.  SR proteins and hnRNP H regulate the splicing of the HIV‐1 tev‐specific exon 6D , 2002, The EMBO journal.

[31]  P. Grabowski,et al.  Mutations in RRM4 uncouple the splicing repression and RNA-binding activities of polypyrimidine tract binding protein. , 2002, RNA.

[32]  D. Brow,et al.  Allosteric cascade of spliceosome activation. , 2002, Annual review of genetics.

[33]  A. Krämer,et al.  Domains in Human Splicing Factors SF3a60 and SF3a66 Required for Binding to SF3a120, Assembly of the 17S U2 snRNP, and Prespliceosome Formation , 2001, Molecular and Cellular Biology.

[34]  M. Adams,et al.  Modulation of P-element pre-mRNA splicing by a direct interaction between PSI and U1 snRNP 70K protein. , 2001, Molecular cell.

[35]  C. Gooding,et al.  Differential alternative splicing activity of isoforms of polypyrimidine tract binding protein (PTB). , 2001, RNA.

[36]  E. Wagner,et al.  Polypyrimidine Tract Binding Protein Antagonizes Exon Definition , 2001, Molecular and Cellular Biology.

[37]  A. Burlingame,et al.  In‐Gel Digestion of Proteins for MALDI‐MS Fingerprint Mapping , 1998, Current protocols in protein science.

[38]  D. Black,et al.  Cooperative Assembly of an hnRNP Complex Induced by a Tissue-Specific Homolog of Polypyrimidine Tract Binding Protein , 2000, Molecular and Cellular Biology.

[39]  Russ P. Carstens,et al.  An Intronic Splicing Silencer Causes Skipping of the IIIb Exon of Fibroblast Growth Factor Receptor 2 through Involvement of Polypyrimidine Tract Binding Protein , 2000, Molecular and Cellular Biology.

[40]  B. Graveley.  Sorting out the complexity of SR protein functions. , 2000, RNA.

[41]  D L Black,et al.  Multisite RNA binding and release of polypyrimidine tract binding protein during the regulation of c-src neural-specific splicing. , 2000, Molecular cell.

[42]  R. Reed,et al.  Mechanisms of fidelity in pre-mRNA splicing. , 2000, Current opinion in cell biology.

[43]  R. Reed,et al.  Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. , 2000, Molecular cell.

[44]  J. Valcárcel,et al.  Inhibition of msl-2 splicing by Sex-lethal reveals interaction between U2AF35 and the 3′ splice site AG , 1999, Nature.

[45]  Benjamin J. Blencowe,et al.  Distinct Factor Requirements for Exonic Splicing Enhancer Function and Binding of U2AF to the Polypyrimidine Tract* , 1999, The Journal of Biological Chemistry.

[46]  R. Reed,et al.  Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels. , 1999, RNA.

[47]  D. Black,et al.  Combinatorial control of a neuron-specific exon. , 1999, RNA.

[48]  C. Gooding,et al.  Polypyrimidine Tract Binding Protein Functions as a Repressor To Regulate Alternative Splicing of α-Actinin Mutally Exclusive Exons , 1999, Molecular and Cellular Biology.

[49]  M. Rosbash,et al.  Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. , 1999, Genes & development.

[50]  D. Helfman,et al.  Polypyrimidine Tract-Binding Protein Positively Regulates Inclusion of an Alternative 3′-Terminal Exon , 1999, Molecular and Cellular Biology.

[51]  Douglas L. Black,et al.  hnRNP H Is a Component of a Splicing Enhancer Complex That Activates a c-src Alternative Exon in Neuronal Cells , 1999, Molecular and Cellular Biology.

[52]  Qing-Shuo Zhang,et al.  RNA-protein interactions , 1999 .

[53]  H. Lou,et al.  Regulation of Alternative Polyadenylation by U1 snRNPs and SRp20 , 1998, Molecular and Cellular Biology.

[54]  Or Gozani,et al.  A Potential Role for U2AF-SAP 155 Interactions in Recruiting U2 snRNP to the Branch Site , 1998, Molecular and Cellular Biology.

[55]  M. Rosbash,et al.  A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition. , 1998, Genes & development.

[56]  C. Guthrie,et al.  Mechanical Devices of the Spliceosome: Motors, Clocks, Springs, and Things , 1998, Cell.

[57]  D L Black,et al.  A complex intronic splicing enhancer from the c-src pre-mRNA activates inclusion of a heterologous exon , 1997, Molecular and cellular biology.

[58]  R. C. Chan,et al.  The polypyrimidine tract binding protein binds upstream of neural cell-specific c-src exon N1 to repress the splicing of the intron downstream , 1997, Molecular and cellular biology.

[59]  M. Rosbash,et al.  Cross-Intron Bridging Interactions in the Yeast Commitment Complex Are Conserved in Mammals , 1997, Cell.

[60]  D L Black,et al.  A new regulatory protein, KSRP, mediates exon inclusion through an intronic splicing enhancer. , 1997, Genes & development.

[61]  S. Berget,et al.  Architectural limits on split genes. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[62]  John E. Coligan,et al.  Current Protocols in Protein Science , 1996 .

[63]  C. Will,et al.  In vitro reconstitution of mammalian U1 snRNPs active in splicing: the U1-C protein enhances the formation of early (E) spliceosomal complexes. , 1996, Nucleic acids research.

[64]  D L Black,et al.  Conserved intron elements repress splicing of a neuron-specific c-src exon in vitro , 1995, Molecular and cellular biology.

[65]  D L Black,et al.  The generally expressed hnRNP F is involved in a neural-specific pre-mRNA splicing event. , 1995, Genes & development.

[66]  J. Valcárcel,et al.  Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. , 1995, Science.

[67]  Jocelyn Côté,et al.  The U1 Small Nuclear Ribonucleoprotein/5′ Splice Site Interaction Affects U2AF65 Binding to the Downstream 3′ Splice Site (*) , 1995, The Journal of Biological Chemistry.

[68]  S. Berget.  Exon Recognition in Vertebrate Splicing (*) , 1995, The Journal of Biological Chemistry.

[69]  J. Steitz,et al.  SR proteins can compensate for the loss of U1 snRNP functions in vitro. , 1994, Genes & development.

[70]  R. Reed,et al.  A functional association between the 5' and 3' splice site is established in the earliest prespliceosome complex (E) in mammals. , 1993, Genes & development.

[71]  Michael R. Green,et al.  The protein Sex-lethal antagonizes the splicing factor U2AF to regulate alternative splicing of transformer pre-mRNA , 1993, Nature.

[72]  P. Sharp,et al.  Splicing of precursors to mRNAs by the spliceosomes , 1993 .

[73]  M. Garcia-Blanco,et al.  The spliceosome assembly pathway in mammalian extracts , 1992, Molecular and cellular biology.

[74]  D. Black.  Activation of c-src neuron-specific splicing by an unusual RNA element in vivo and in vitro , 1992, Cell.

[75]  R. Reed,et al.  An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway. , 1991, Genes & development.

[76]  J. Dignam.  Preparation of extracts from higher eukaryotes. , 1990, Methods in enzymology.

[77]  J. Brugge,et al.  The structurally distinct form of pp60c-src detected in neuronal cells is encoded by a unique c-src mRNA , 1987, Molecular and cellular biology.

[78]  P. Sharp,et al.  A mutational analysis of spliceosome assembly: evidence for splice site collaboration during spliceosome formation. , 1987, Genes & development.

[79]  D. Baltimore,et al.  Neuronal pp60c-src contains a six-amino acid insertion relative to its non-neuronal counterpart. , 1987, Science.

[80]  J. Steitz,et al.  U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing , 1985, Cell.

[81]  M. Wilm,et al.  Characterization of novel SF 3 b and 17 S U 2 snRNP proteins , including a human Prp 5 p homologue and an SF 3 b DEAD-box protein , 2022 .