Efficiency of different strategies for the control of grey mold on grapes including gibberellic acid (Gibb3), leaf removal and/or botrycide treatments

Aim: The present work evaluated different strategies for the control of grey mold, caused by Botrytis cinerea, on wine grapes, including the use of the plant growth regulator Gibb3, leaf removal and/or botryticide treatments. The efficiency of the different control strategies (disease incidence and severity, yield) as well as the effect on the cluster structure was investigated.Methods and results: The trials were conducted in commercial vineyards in the Moselle Valley (Luxembourg) between the years 2007 and 2009, on the Pinot gris, Pinot blanc and Pinot noir grape varieties. The untreated control (T1) was compared to the following treatments : (T2) Gibb3, (T3) Gibb3 combined with leaf removal in the cluster zone after bloom, (T4) leaf removal after bloom combined with two times botryticides and (T5) Gibb3 combined with two times botryticides. The combination of Gibb3 with leaf removal or botryticide applications led to an efficiency level in grey mold reduction of around 60% and a decrease in cluster density when compared to the control. Moreover, we showed that the progression of grey mold disease was slowed down by the three treatments T3, T4 and T5.Conclusions: Gibberellic acid applied at full bloom as stand-alone treatment did not reduce in a significant way the compactness of the grape clusters and the impact on grey mold development was low. For a significant decrease of disease severity, gibberellic acid had to be combined with an additional measure, such as leaf removal or the use of botryticides. Based on its positive effect on cluster structure and microclimate, leaf removal can be recommended as a basic measure that can be further combined with an application of botryticides or gibberellic acid.Significance and impact of the study: All the tested strategies combining two measures have shown their potential to prolong the ripening period and therewith to improve wine quality. Moreover, the combined use of gibberellic acid and leaf removal represents a sustainable strategy for integrated viticulture due to its reduced input of organic-synthetic pesticides into the environment.

[1]  M. Trought,et al.  The Flowering Process of Vitis vinifera: A Review , 2009, American Journal of Enology and Viticulture.

[2]  S. Fillinger,et al.  Genetic Analysis of Fenhexamid-Resistant Field Isolates of the Phytopathogenic Fungus Botrytis cinerea , 2008, Antimicrobial Agents and Chemotherapy.

[3]  Stefano Poni,et al.  Early defoliation (hand vs mechanical) for improved crop control and grape composition in Sangiovese (Vitis vinifera L.) , 2008 .

[4]  S. Wratten,et al.  Understorey management increases grape quality, yield and resistance to Botrytis cinerea , 2007 .

[5]  P. Elmer,et al.  Biosuppression of Botrytis cinerea in grapes , 2006 .

[6]  J. Casado-Vela,et al.  Modified cyclodextrins are chemically defined glucan inducers of defense responses in grapevine cell cultures. , 2006, Journal of agricultural and food chemistry.

[7]  H. Schultz,et al.  PHYSIOLOGICAL RESPONSES OF MINIMAL PRUNING SYSTEMS TO GIBBERELLIC ACID , 2005 .

[8]  P. Pieri,et al.  EFFECTS OF DEFOLIATION ON TEMPERATURE AND WETNESS OF GRAPEVINE BERRIES , 2005 .

[9]  D. Dubourdieu,et al.  Origin of (−)-Geosmin on Grapes: On the Complementary Action of Two Fungi, Botrytis Cinerea and Penicillium Expansum , 2005, Antonie van Leeuwenhoek.

[10]  M. Gullino,et al.  Improving the efficacy of biocontrol agents against soilborne pathogens , 2005 .

[11]  Mara Rossoni,et al.  Benzothiadiazole enhances resveratrol and anthocyanin biosynthesis in grapevine, meanwhile improving resistance to Botrytis cinerea. , 2004, Journal of agricultural and food chemistry.

[12]  B. Zoecklein,et al.  Effects of Prohexadione-calcium on Grape Yield Components and Fruit and Wine Composition , 2004, American Journal of Enology and Viticulture.

[13]  A. Heyraud,et al.  Oligogalacturonide signal transduction, induction of defense-related responses and protection of grapevine against Botrytis cinerea , 2004, Planta.

[14]  B. Poinssot,et al.  Laminarin elicits defense responses in grapevine and induces protection against Botrytis cinerea and Plasmopara viticola. , 2003, Molecular plant-microbe interactions : MPMI.

[15]  Franka Mlikota Gabler,et al.  Correlations of Morphological, Anatomical, and Chemical Features of Grape Berries with Resistance to Botrytis cinerea. , 2003, Phytopathology.

[16]  K. Takemura,et al.  Effects of the Combination of Gibberellic Acid and Ammonium Nitrate on the Growth and Quality of Seedless Berries in 'Delaware' Grape , 2003 .

[17]  B. Tudzynski Biosynthesis of gibberellins in Gibberella fujikuroi: biomolecular aspects , 1999, Applied Microbiology and Biotechnology.

[18]  N. Ollat,et al.  The Effect of Limiting Leaf Area During Stage I of Berry Growth on Development and Composition of Berries of Vitis vinifera L. cv. Cabernet Sauvignon , 1998, American Journal of Enology and Viticulture.

[19]  D. Shtienberg,et al.  Incorporation of Weather Forecasting in Integrated, Biological-Chemical Management of Botrytis cinerea. , 1997, Phytopathology.

[20]  H. Bleiholder,et al.  Growth Stages of the Grapevine: Phenological growth stages of the grapevine (Vitis vinifera L. ssp. vinifera)—Codes and descriptions according to the extended BBCH scale† , 1995 .

[21]  B. Zoecklein,et al.  Effects of Fruit Zone Leaf Removal on Yield, Fruit Composition, and Fruit Rot Incidence of Chardonnay and White Riesling (Vitis viniferaL.) Grapes , 1992, American Journal of Enology and Viticulture.

[22]  J. Marois,et al.  Grape cluster architecture and the susceptibility of berries to Botrytis cinerea , 1991 .

[23]  J. Marois,et al.  Microclimates of grapevine canopies associated with leaf removal and control of Botrytis bunch rot , 1989 .

[24]  J. Marois,et al.  Control of Botrytis bunch rot of grape with canopy management , 1987 .

[25]  M. Sall,et al.  Botrytis Bunch Rot of Grapes: Influence of Trellis Type and Canopy Microclimate , 1984 .

[26]  R. Weaver,et al.  Studies with Gibberellin on Wine Grapes to Decrease Bunch Rot , 1962, American Journal of Enology and Viticulture.