Movement within and movement beyond

Leukocyte chemotaxis plays an essential role in generating and delivering immune responses and is a critical component of inflammation. In order to identify novel genes and pathways important for regulating chemotaxis, we performed an RNAi-based screen and identified several genes involved with vesicle movement and fusion as mediators of chemotaxis. Our recently published data1 show that during chemotaxis vesicle trafficking proteins are required for lysosome fusion, uropod release and efficient directed cell migration.

[1]  N. Hacohen,et al.  Synaptotagmin-mediated vesicle fusion regulates cell migration , 2010, Nature Immunology.

[2]  A. Viola,et al.  Chemokines and their receptors: drug targets in immunity and inflammation. , 2008, Annual review of pharmacology and toxicology.

[3]  R. Kay,et al.  Possible roles of the endocytic cycle in cell motility , 2007, Journal of Cell Science.

[4]  T. Galli,et al.  Expression of the Longin domain of TI‐VAMP impairs lysosomal secretion and epithelial cell migration , 2007, Biology of the cell.

[5]  H. Janssen,et al.  Synaptotagmin 3 deficiency in T cells impairs recycling of the chemokine receptor CXCR4 and thereby inhibits CXCL12 chemokine-induced migration , 2007, Journal of Cell Science.

[6]  B. A. Ballif,et al.  Phosphorylation of ACAP1 by Akt regulates the stimulation-dependent recycling of integrin beta1 to control cell migration. , 2005, Developmental cell.

[7]  C. Huynh,et al.  Identification of SNAREs Involved in Synaptotagmin VII-regulated Lysosomal Exocytosis* , 2004, Journal of Biological Chemistry.

[8]  K. Mikoshiba,et al.  Synaptotagmin-like protein 5: a novel Rab27A effector with C-terminal tandem C2 domains. , 2002, Biochemical and biophysical research communications.

[9]  R. Sagi-Eisenberg,et al.  Synaptotagmin II Negatively Regulates Ca2+-triggered Exocytosis of Lysosomes in Mast Cells , 1999, The Journal of experimental medicine.

[10]  D. Strickland,et al.  In migrating fibroblasts, recycling receptors are concentrated in narrow tubules in the pericentriolar area, and then routed to the plasma membrane of the leading lamella , 1994, The Journal of cell biology.

[11]  K. Jacobson,et al.  The direction of membrane lipid flow in locomoting polymorphonuclear leukocytes. , 1990, Science.

[12]  J. Fehr,et al.  Modulating influence of chemotactic factor-induced cell adhesiveness on granulocyte function. , 1979, The Journal of clinical investigation.

[13]  Becker El.  Some interrelations of neutrophil chemotaxis, lysosomal enzyme secretion, and phagocytosis as revealed by synthetic peptides. , 1976 .

[14]  H. Showell,et al.  The ability of chemotactic factors to induce lysosomal enzyme release. II. The mechanism of release. , 1974, Journal of immunology.

[15]  P. Henson,et al.  The ability of chemotactic factors to induce lysosomal enzyme release. I. The characteristics of the release, the importance of surfaces and the relation of enzyme release to chemotactic responsiveness. , 1974, Journal of immunology.

[16]  M. Abercrombie,et al.  The locomotion of fibroblasts in culture. 3. Movements of particles on the dorsal surface of the leading lamella. , 1970, Experimental cell research.

[17]  M. Abercrombie,et al.  The locomotion of fibroblasts in culture. II. "RRuffling". , 1970, Experimental cell research.

[18]  M. Abercrombie,et al.  The locomotion of fibroblasts in culture. I. Movements of the leading edge. , 1970, Experimental cell research.

[19]  P. I. Marcus.  Dynamics of surface modification in myxovirus-infected cells. , 1962, Cold Spring Harbor symposia on quantitative biology.