Recognition of Different Types of Leukocytes Using YOLOv2 and Optimized Bag-of-Features

White blood cells (WBCs) protect human body against different types of infections including fungal, parasitic, viral, and bacterial. The detection of abnormal regions in WBCs is a difficult task. Therefore a method is proposed for the localization of WBCs based on YOLOv2-Nucleus-Cytoplasm, which contains darkNet-19 as a basenetwork of the YOLOv2 model. In this model features are extracted from LeakyReLU-18 of darkNet-19 and supplied as an input to the YOLOv2 model. The YOLOv2-Nucleus-Cytoplasm model localizes and classifies the WBCs with maximum score labels. It also localize the WBCs into the blast and non-blast cells. After localization, the bag-of-features are extracted and optimized by using particle swarm optimization(PSO). The improved feature vector is fed to classifiers i.e., optimized naïve Bayes (O-NB) & optimized discriminant analysis (O-DA) for WBCs classification. The experiments are performed on LISC, ALL-IDB1, and ALL-IDB2 datasets.

[1]  B. Walczak,et al.  Particle swarm optimization (PSO). A tutorial , 2015 .

[2]  Cong Wang,et al.  CycleGAN With an Improved Loss Function for Cell Detection Using Partly Labeled Images , 2020, IEEE Journal of Biomedical and Health Informatics.

[3]  Ali Farhadi,et al.  YOLO9000: Better, Faster, Stronger , 2016, 2017 IEEE Conference on Computer Vision and Pattern Recognition (CVPR).

[4]  Keerthana Prasad,et al.  Comparison of traditional image processing and deep learning approaches for classification of white blood cells in peripheral blood smear images , 2019, Biocybernetics and Biomedical Engineering.

[5]  Vincenzo Piuri,et al.  All-IDB: The acute lymphoblastic leukemia image database for image processing , 2011, 2011 18th IEEE International Conference on Image Processing.

[6]  Zuoyong Li,et al.  LeukocyteMask: An automated localization and segmentation method for leukocyte in blood smear images using deep neural networks , 2019, Journal of biophotonics.

[7]  V. Piuri,et al.  Morphological classification of blood leucocytes by microscope images , 2004, 2004 IEEE International Conference onComputational Intelligence for Measurement Systems and Applications, 2004. CIMSA..

[8]  Nancy M. Salem,et al.  A Comparative Study of White Blood cells Segmentation using Otsu Threshold and Watershed Transformation , 2016 .

[9]  Ehdi,et al.  AUTOMATIC OBJECT DETECTION AND SEGMENTATION OF THE HISTOCYTOLOGY IMAGES USING RESHAPABLE AGENTS , 2013 .

[10]  Cecilia Di Ruberto,et al.  Leucocyte classification for leukaemia detection using image processing techniques , 2014, Artif. Intell. Medicine.

[11]  Yudong Zhang,et al.  Polarimetric synthetic aperture radar image segmentation by convolutional neural network using graphical processing units , 2017, Journal of Real-Time Image Processing.

[12]  Arun Kumar Sangaiah,et al.  Alcoholism identification via convolutional neural network based on parametric ReLU, dropout, and batch normalization , 2018, Neural Computing and Applications.

[13]  Sung Wook Baik,et al.  Leukocytes Classification and Segmentation in Microscopic Blood Smear: A Resource-Aware Healthcare Service in Smart Cities , 2017, IEEE Access.

[14]  Shipeng Xie,et al.  Alcoholism Identification Based on an AlexNet Transfer Learning Model , 2019, Front. Psychiatry.

[15]  D. Ruppert The Elements of Statistical Learning: Data Mining, Inference, and Prediction , 2004 .

[16]  Mohd Razali Md Tomari,et al.  White blood cell (WBC) counting analysis in blood smear images using various color segmentation methods , 2018 .

[17]  Partha Pratim Banik,et al.  An Automatic Nucleus Segmentation and CNN Model based Classification Method of White Blood Cell , 2020, Expert Syst. Appl..

[18]  Vittorio Rizzoli,et al.  Bleomycin genotoxicity and amifostine (WR-2721) cell protection in normal leukocytes vs. K562 tumoral cells. , 2002, Biochemical pharmacology.

[19]  E. Crimmins,et al.  Inflammation as a potential mediator for the association between periodontal disease and Alzheimer’s disease , 2008, Neuropsychiatric disease and treatment.

[20]  Engin Avci,et al.  White blood cells detection and classification based on regional convolutional neural networks. , 2019, Medical hypotheses.

[21]  Leyza Baldo Dorini,et al.  White blood cell segmentation using morphological operators and scale-space analysis , 2007, XX Brazilian Symposium on Computer Graphics and Image Processing (SIBGRAPI 2007).

[22]  R. Venkatesan,et al.  Interpolative Leishman-Stained transformation invariant deep pattern classification for white blood cells , 2020, Soft Comput..

[23]  Bin Liu,et al.  Unilateral sensorineural hearing loss identification based on double-density dual-tree complex wavelet transform and multinomial logistic regression , 2019, Integr. Comput. Aided Eng..

[24]  B. Mehta,et al.  Leukocyte-Related Disorders: A Review for the Pediatrician. , 2020, Pediatric annals.

[25]  Cecilia Di Ruberto,et al.  A leucocytes count system from blood smear images , 2016, Machine Vision and Applications.

[26]  Hamdan O. Alanazi,et al.  An Automated White Blood Cell Nucleus Localization and Segmentation using Image Arithmetic and Automatic Threshold , 2010 .

[27]  Yan Li,et al.  Segmentation of White Blood Cell from Acute Lymphoblastic Leukemia Images Using Dual-Threshold Method , 2016, Comput. Math. Methods Medicine.

[28]  Shuihua Wang,et al.  Cerebral Micro-Bleeding Detection Based on Densely Connected Neural Network , 2019, Front. Neurosci..

[29]  David J. Cappelleri,et al.  Automated Complete Blood Cell Count and Malaria Pathogen Detection Using Convolution Neural Network , 2020, IEEE Robotics and Automation Letters.

[30]  Tanzila Saba,et al.  A New Approach for Brain Tumor Segmentation and Classification Based on Score Level Fusion Using Transfer Learning , 2019, Journal of Medical Systems.

[31]  N. Young,et al.  Current concepts in the pathophysiology and treatment of aplastic anemia. , 2013, Hematology. American Society of Hematology. Education Program.

[32]  Joel H. Saltz,et al.  Methods for Segmentation and Classification of Digital Microscopy Tissue Images , 2018, Front. Bioeng. Biotechnol..

[33]  K. V. Arya,et al.  Automated microscopic image analysis for leukocytes identification: a survey. , 2014, Micron.

[34]  R. Fisher THE USE OF MULTIPLE MEASUREMENTS IN TAXONOMIC PROBLEMS , 1936 .

[35]  Tolga Tasdizen,et al.  Isolation and two-step classification of normal white blood cells in peripheral blood smears , 2012, Journal of pathology informatics.

[36]  Khachik Sargsyan,et al.  Pathology of porcine peripheral white blood cells during infection with African swine fever virus , 2012, BMC Veterinary Research.

[37]  Jianming Zhang,et al.  A Real-Time Chinese Traffic Sign Detection Algorithm Based on Modified YOLOv2 , 2017, Algorithms.

[38]  F. Scotti,et al.  Automatic morphological analysis for acute leukemia identification in peripheral blood microscope images , 2005, CIMSA. 2005 IEEE International Conference on Computational Intelligence for Measurement Systems and Applications, 2005..

[39]  Yusuf Yargı Baydilli,et al.  Classification of white blood cells using capsule networks , 2020, Comput. Medical Imaging Graph..

[40]  Miss. Madhuri G. Bhamare,et al.  Automatic Blood Cell Analysis by Using Digital Image Processing: A Preliminary Study , 2013 .

[41]  Changyan Xiao,et al.  Simultaneous Segmentation of Leukocyte and Erythrocyte in Microscopic Images Using a Marker-Controlled Watershed Algorithm , 2018, Comput. Math. Methods Medicine.

[42]  Ming Yang,et al.  Multivariate Approach for Alzheimer's Disease Detection Using Stationary Wavelet Entropy and Predator-Prey Particle Swarm Optimization. , 2018, Journal of Alzheimer's disease : JAD.

[43]  F. Scotti,et al.  Robust Segmentation and Measurements Techniques of White Cells in Blood Microscope Images , 2006, 2006 IEEE Instrumentation and Measurement Technology Conference Proceedings.

[44]  Xiaomei Li,et al.  Segmentation of White Blood Cells through Nucleus Mark Watershed Operations and Mean Shift Clustering , 2015, Sensors.

[45]  Keerthana Prasad,et al.  Automated Decision Support System for Detection of Leukemia from Peripheral Blood Smear Images , 2019, Journal of Digital Imaging.

[46]  Yingli Tian,et al.  A Heuristic Neural Network Structure Relying on Fuzzy Logic for Images Scoring , 2020, IEEE Transactions on Fuzzy Systems.

[47]  Gabriela Csurka,et al.  Visual categorization with bags of keypoints , 2002, eccv 2004.

[48]  Leyza Baldo Dorini,et al.  Semiautomatic White Blood Cell Segmentation Based on Multiscale Analysis , 2013, IEEE Journal of Biomedical and Health Informatics.

[49]  Mohammad A. Qasaimeh,et al.  Cell Cytometry: Review and Perspective on Biotechnological Advances , 2019, Front. Bioeng. Biotechnol..

[50]  Yudong Zhang,et al.  Cerebral micro‐bleeding identification based on a nine‐layer convolutional neural network with stochastic pooling , 2019, Concurr. Comput. Pract. Exp..

[51]  Asifullah Khan,et al.  A survey of the recent architectures of deep convolutional neural networks , 2019, Artificial Intelligence Review.

[52]  Hai-Quan Vu,et al.  Cell Splitting with High Degree of Overlapping in Peripheral Blood Smear , 2011 .

[53]  Junding Sun,et al.  High Performance Multiple Sclerosis Classification by Data Augmentation and AlexNet Transfer Learning Model , 2019, J. Medical Imaging Health Informatics.

[54]  Dixit Kolhatkar,et al.  Detection and counting of blood cells using image segmentation: A review , 2016, 2016 World Conference on Futuristic Trends in Research and Innovation for Social Welfare (Startup Conclave).

[55]  J. E. Lowther Compressibility of highly coordinated metal oxynitrides : LDA calculations , 2005 .

[56]  Muhammad Shahzad,et al.  Robust Method for Semantic Segmentation of Whole-Slide Blood Cell Microscopic Images , 2020, Comput. Math. Methods Medicine.