Decolorization of textile dyes and their effluents using white rot fungi

Reactive dyes are important chemical pollutants from textile industries .The two species of white rot fungi were evaluated for their ability to decolorize Blue CA, Black B133, Corazol Violet SR. Trametes hirsuta and Pleurotus florida displayed the greatest extent of decolorization. Laccase is the ligneolytic enzyme from these fungi. The laccase activity was measured using both solid and aqueous state assays. The dye absorption ability of the mycelium was studied using appropriate medium containing dyes at the concentration of 75 mg/l. The effective decolorization of Blue CA and Corazol Violet SR dyes by both microorganisms were observed in the fifth day of incubation. Further decolorization activity was verified using various concentrations of dyes such as 25, 50 and 75 mg/l. Maximum decolorization was observed in Blue CA and Corazol Violet SR dyes. The effluent from the dye house was treated using both organisms with different concentration of glucose (1 and 2%). Effective decolourization was found to be more by the Pleurotus florida in 2% glucose.

[1]  M. Capelari,et al.  Lignin degradation andIn vitro digestibility of wheat straw treated with Brazilian tropical species of white rot fungi , 1997, Folia Microbiologica.

[2]  E. A. Erkurt,et al.  Decolorization of synthetic dyes by white rot fungi, involving laccase enzyme in the process , 2007 .

[3]  Young-Mo Kim,et al.  Purification and characterization of laccase produced by a white rot fungus Pleurotus sajor-caju under submerged culture condition and its potential in decolorization of azo dyes , 2006, Applied Microbiology and Biotechnology.

[4]  P. Baldrian,et al.  Decolorization of structurally different synthetic dyes using cobalt(II)/ascorbic acid/hydrogen peroxide system. , 2003, Chemosphere.

[5]  K. Patel,et al.  Decolorization of synthetic dyes by Irpex lacteus in liquid cultures and packed-bed bioreactor , 2003 .

[6]  V. Šašek,et al.  Capacity of Irpex lacteus and Pleurotus ostreatus for decolorization of chemically different dyes. , 2001, Journal of biotechnology.

[7]  A. Kandelbauer,et al.  Indigo degradation with purified laccases from Trametes hirsuta and Sclerotium rolfsii. , 2001, Journal of biotechnology.

[8]  Jo‐Shu Chang,et al.  Decolorization of azo dyes with immobilized Pseudomonas luteola , 2001 .

[9]  A. López-Munguía,et al.  Residual compost of Agaricus bisporus as a source of crude laccase for enzymic oxidation of phenolic compounds , 2001 .

[10]  T. Cajthaml,et al.  Irpex lacteus, a white rot fungus applicable to water and soil bioremediation , 2000, Applied Microbiology and Biotechnology.

[11]  F. Schinner,et al.  Characterization and immobilization of the laccase from Pleurotus ostreatus and its use for the continuous elimination of phenolic pollutants. , 2000, Enzyme and microbial technology.

[12]  T. Tzanov,et al.  Decolorization and Detoxification of Textile Dyes with a Laccase from Trametes hirsuta , 2000, Applied and Environmental Microbiology.

[13]  V. Šašek,et al.  Biological decolorization of the synthetic dye RBBR in contaminated soil , 2000 .

[14]  J. Knapp,et al.  The decolourisation of a chemical industry effluent by white rot fungi , 1999 .

[15]  J. Ramsay,et al.  The evaluation of white rot fungi in the decoloration of textile dyes , 1999 .

[16]  M. Pickard,et al.  Industrial Dye Decolorization by Laccases from Ligninolytic Fungi , 1999, Current Microbiology.

[17]  R. Sani,et al.  Biodegradation of triphenylmethane dyes. , 1998, Enzyme and microbial technology.

[18]  S. Pavlostathis,et al.  Decolorization and toxicity screening of selected reactive azo dyes under methanogenic conditions , 1998 .

[19]  Ibrahim M. Banat,et al.  Microbial decolorization of textile-dye-containing effluents A review , 1996 .

[20]  J. Knapp,et al.  Decolorization of dyes by wood-rotting basidiomycete fungi , 1995 .

[21]  R. Crawford,et al.  Potential for Bioremediation of Xenobiotic Compounds by the White‐Rot Fungus Phanerochaete chrysosporium , 1995 .

[22]  M. Shoda,et al.  Characteristics of a newly isolated fungus, Geotrichum candidum Dec 1, which decolorizes various dyes , 1995 .

[23]  C. A. Reddy,et al.  The potential for white-rot fungi in the treatment of pollutants , 1995 .

[24]  S. Stevens,et al.  Degradation azo dyes by environmental microorganisms and helminths , 1993 .

[25]  H. Zollinger Color chemistry: Syntheses, properties, and applications of organic dyes and pigments , 1987 .

[26]  R. Farrell,et al.  Enzymatic "combustion": the microbial degradation of lignin. , 1987, Annual review of microbiology.

[27]  R. Willson,et al.  Radical-cations as reference chromogens in kinetic studies of ono-electron transfer reactions: pulse radiolysis studies of 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) , 1982 .

[28]  M. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[29]  M. Alexander,et al.  Effect of Chemical Structure on Microbial Degradation of Substituted Benzenes , 1966 .