A Novel Approach for the Identification of Morphological Features from Low Quality Images

In this paper, a novel mathematical morphological approach is proposed, which is combined with an active threshold-based method for the identification of morphological features from images with poor qualities. The algorithm is very fast and needs low computing power. First, a mixed smooth filtering is designed to remove background noises. Second, an active threshold-based method is discussed to create a binary image to achieve rough segmentation. Third, some simple morphological operations, such as opening, closing, filling, and so on, are designed and applied to get the final result of segmentation. After morphological analysis, morphological features, such as contours, areas, numbers, locations, and so on, are obtained. Finally, the comparisons with other conventional methods validate the effectiveness, and an additional experimental result proves the repeatability of the proposed method.

[1]  Melvyn L. Smith,et al.  A mathematical morphology approach to image based 3D particle shape analysis , 2005, Machine Vision and Applications.

[2]  Theodosios Pavlidis,et al.  Integrating Region Growing and Edge Detection , 1990, IEEE Trans. Pattern Anal. Mach. Intell..

[3]  Behzad Shahraray,et al.  Uniform Resampling of Digitized Contours , 1985, IEEE Transactions on Pattern Analysis and Machine Intelligence.

[4]  D Marr,et al.  Theory of edge detection , 1979, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[5]  R. Wingate,et al.  Imagining the brain cell: the neuron in visual culture , 2006, Nature Reviews Neuroscience.

[6]  Hugues Talbot,et al.  Mathematical morphology: A useful set of tools for image analysis , 2000, Stat. Comput..

[7]  P.K Sahoo,et al.  A survey of thresholding techniques , 1988, Comput. Vis. Graph. Image Process..

[8]  Andrew P. Witkin,et al.  Scale-Space Filtering , 1983, IJCAI.

[9]  John F. Canny,et al.  A Computational Approach to Edge Detection , 1986, IEEE Transactions on Pattern Analysis and Machine Intelligence.

[10]  D. Leland,et al.  Role of Cell Culture for Virus Detection in the Age of Technology , 2007, Clinical Microbiology Reviews.

[11]  Dorin Comaniciu,et al.  Mean shift analysis and applications , 1999, Proceedings of the Seventh IEEE International Conference on Computer Vision.

[12]  Victoria J Allan,et al.  Light Microscopy Techniques for Live Cell Imaging , 2003, Science.

[13]  J. Serra Introduction to mathematical morphology , 1986 .

[14]  J. Lyman,et al.  Updated Review of Blood Culture Contamination , 2006, Clinical Microbiology Reviews.

[15]  N. Otsu A threshold selection method from gray level histograms , 1979 .

[16]  Henk J. A. M. Heijmans,et al.  Nonlinear multiresolution signal decomposition schemes. I. Morphological pyramids , 2000, IEEE Trans. Image Process..

[17]  Michael L. Dustin,et al.  Dynamic imaging of the immune system: progress, pitfalls and promise , 2006, Nature Reviews Immunology.

[18]  Stéphane Mallat,et al.  Multifrequency channel decompositions of images and wavelet models , 1989, IEEE Trans. Acoust. Speech Signal Process..

[19]  Abderrahim Elmoataz,et al.  Using active contours and mathematical morphology tools for quantification of immunohistochemical images , 1998, Signal Process..

[20]  Yizong Cheng,et al.  Mean Shift, Mode Seeking, and Clustering , 1995, IEEE Trans. Pattern Anal. Mach. Intell..

[21]  Stéphane Mallat,et al.  A Theory for Multiresolution Signal Decomposition: The Wavelet Representation , 1989, IEEE Trans. Pattern Anal. Mach. Intell..