In recent years, a number of research groups have begun developing nanofabrication methods based on DNA self‐assembly. Here we review our recent experimental progress to utilize novel DNA nanostructures for self‐assembly as well as for templates in the fabrication of functional nano‐patterned materials. We have prototyped a new DNA nanostructure known as a cross structure. This nanostructure has a 4‐fold symmetry which promotes its self‐assembly into tetragonal 2D lattices. We have utilized the tetragonal 2D lattices as templates for highly conductive metallic nanowires and periodic 2D protein nano‐arrays. We have constructed and characterized a DNA nanotube, a new self‐assembling superstructure composed of DNA tiles. We have also demonstrated an aperiodic DNA lattice composed of DNA tiles assembled around a long scaffold strand; the system translates information encoded in the scaffold strand into a specific and reprogrammable barcode pattern. We have achieved metallic nanoparticle linear arrays template...