论文信息 - Synthesis of oligodeoxyribonucleotides containing 2'-deoxypseudouridine: inhibition of uracil-DNA glycosylase.

Synthesis of oligodeoxyribonucleotides containing 2'-deoxypseudouridine: inhibition of uracil-DNA glycosylase.

2'-Deoxypseudouridine (1) was synthesized starting from thymidine in a 23% overall yield via a silylated furanoid glycal. Compound 1 was introduced to oligodeoxyribonucleotides (ODNs) by the solid phase phosphoramidite chemistry. The ODNs containing 1 inhibited uracil-DNA glycosylase (UDG) reaction.

A. Ono | A. Nishizima

[1] G. Gilliland,et al. Crystal structure of Escherichia coli uracil DNA glycosylase and its complexes with uracil and glycerol: Structure and glycosylase mechanism revisited , 1999, Proteins.