13C metabolic flux analysis in cell line and bioprocess development

With an ever-increasing demand for biopharmaceuticals, there is a growing need for high-producing industrial cell lines. 13C metabolic flux analysis provides a comprehensive perspective of host metabolism by rigorously quantifying fluxes within intracellular metabolic networks. The characterization of production cell lines altered via metabolic engineering or media optimization can help to identify desirable metabolic phenotypes. Coupled with the advent of high-throughput mini bioreactor systems and expanded metabolic modeling capabilities, 13C flux analysis can provide an even more complete picture of intracellular metabolism. The resulting knowledge of desirable cellular traits and metabolic regulatory nodes offers new opportunities for the systematic identification of engineering targets to develop optimized cell lines and bioprocesses.

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