论文信息 - Colorimetric Determination of Surfactants Using Lipase

Colorimetric Determination of Surfactants Using Lipase

Abstract The inhibition effect of a surfactant on an enzymic reaction of lipase was investigated to determine small amounts of the surfactant. Surfactants could be determined by colori-metrically measuring the degree of their inhibition in the enzymic reaction. By the method studied, 10–200 ppm of LAS, 25–300 ppm of ABS, 102 −103 ppm of SDS, and 1–10% of polyethyleneglycol dodecyl ether and polyethylene glycol octylphenyl ether could be determined with coefficient of variance of about 2%.

K. Konishi | H. Tanaka | K. Tsuji

[1] T. Ogiso,et al. [Studies on bile-sensitive lipase. VII. Effect of surfactants on Mucor lipase (studies on enzymes. 48)]. , 1969, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan.

[2] M. H. Sadar,et al. Fluorometric determination of pesticides. , 1969, Analytical chemistry.

[3] G G Guilbault,et al. Fluorometric system employing immobilized cholinesterase for assaying anticholinesterase compounds. , 1965, Analytical chemistry.

[4] G. Guilbault,et al. Fluorometric Determination of Lipase, Acylase, Alpha-, and Gamma-Chymotrypsin and Inhibitors of These Enzymes. , 1964 .

[5] G. Guilbault,et al. Electrical Determination of Organophosphorous Compounds. , 1962 .

[6] D. C. Abbott. The colorimetric determination of anionic surface-active materials in water , 1962 .

[7] E. A. Lazo-wasem. Determination of Lipase Activity in Pharmaceutical Preparations Intended for Oral Administration , 1961 .

[8] E. Wills. The effect of surface-active agents on pancreatic lipase. , 1955, The Biochemical journal.

[9] E. Wills. The effect of anionic detergents and some related compounds on enzymes. , 1954, The Biochemical journal.

[10] J. Lapides,et al. CHROMOGENIC SUBSTRATES IV. ACYL ESTERS OF p-NITROPHENOL AS SUBSTRATES FOR THE COLORIMETRIC DETERMINATION OF ESTERASE , 1947 .