Monocotyledonous Plants as Hosts for Agrobacterium

Fifteen species from nine monocotyledonous families were inoculated with strains of Agrobacterium tumefaciens, A. rhizogenes, and A. rubi. Tumor production was observed in seven of these species: Arthropodium cirratum (New Zealand rock lily), Cordyline australis (New Zealand cabbage tree), Dioscorea bulbifera (yam), Monstera deliciosa (fruit salad plant), Nerine bowdenii, Polygonatum x hybridum (Solomon's seal), and Zantedeschia aethiopica (arum lily). The use of appropriate controls for Agrobacterium inoculations and the detection of opines in extracts from most of these tumors confirmed that they had resulted from Agrobacterium-mediated gene transfer. Chlorophyll retention around tumors during senescence in some of the plant species was attributed to the expression of the T-DNA isopentyl-transferase gene. We conclude that more monocotyledons are hosts for Agrobacterium transformation than previously thought. Important factors for the induction of tumors included the choice of Agrobacterium strain, the growth phase of the plant, and the organ being inoculated.

[1]  N. Sugii.  Induction of Tumors in Anthurium andraeanum by Agrobactemium , 1991 .

[2]  S. Gelvin.  Crown gall disease and hairy root disease : a sledgehammer and a tackhammer. , 1990, Plant physiology.

[3]  C. Bhatia,et al.  Agrobacterium Tumefaciens-induced tumour formation on some tropical dicot and monocot plants , 1987 .

[4]  M. Montagu,et al.  T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[5]  G. Kahl,et al.  T-DNA integration and expression in a monocot crop plant after induction of Agrobacterium , 1987, Nature.

[6]  S. Goldman,et al.  Agrobacterium tumefaciens-mediated transformation of the monocot genus Gladiolus: detection of expression of T-DNA-encoded genes , 1987, Journal of bacteriology.

[7]  M. Cleene.  The Susceptibility of Monocotyledons to Agrobacterium tumefaciens , 1985 .

[8]  M. Van Montagu,et al.  An Agrobacterium‐transformed cell culture from the monocot Asparagus officinalis , 1984, The EMBO journal.

[9]  P. Hooykaas,et al.  Expression of Ti plasmid genes in monocotyledonous plants infected with Agrobacterium tumefaciens , 1984, Nature.

[10]  P. Hirsch,et al.  A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid , 1983, Nature.

[11]  D. Garfinkel,et al.  Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism , 1980, Journal of bacteriology.

[12]  G. Strobel,et al.  Involvement of a plasmid in the hairy root disease of plants caused by Agrobacterium rhizogenes. , 1979, Plasmid.

[13]  M. Chilton,et al.  Fingerprints of Agrobacterium Ti plasmids. , 1978, Plasmid.

[14]  Kenneth V. Thimann,et al.  Plant Hormones. (Book Reviews: Hormone Action in the Whole Life of Plants) , 1977 .

[15]  M. Chilton,et al.  Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[16]  A. J. Conner,et al.  Gene transfer to plants using Agrobacterium. , 1991 .

[17]  A. J. Conner,et al.  Onion is a monocotyledonous host for Agrobacterium , 1990 .

[18]  J. Greef,et al.  Functional Expression of Agrobacterium tumefaciens T-DNA onc-genes in Asparagus Crown Gall Tissues , 1990 .

[19]  M. Gordon,et al.  Agrobacterium–Mediated Transformation of Rice (Oryza sativa L.) , 1990, Bio/Technology.

[20]  Q. Hao.  EXPRESSION OF Ti PLASMID GENES IN MONOCOTYLEDONOUS PLANTS OF Hippeastrum rutilum AND Chlorophytum comosum , 1988 .

[21]  J. Matoušek,et al.  Differentiation of transformed plants from tumors induced by Agrobacterium rubi ATCC 13335 , 1987 .

[22]  N. Grimsley,et al.  Agrobacterium-mediated delivery of infectious maize streak virus into maize plants , 1987, Nature.

[23]  L. Moore,et al.  Host specificity in the genus Agrobacterium. , 1979 .