[Effect of konjac polysaccharide on levels of leptin and Na+ -K+-ATPase of mice treated with high fat].

OBJECTIVE To investigate the effect of konjac polysaccharide on serum leptin and intestinal mucosa Na+ -K+-ATPase activity of mice treated with high fat. METHODS Mice were divided into normal control, high-fat control, and high, middle, low dose of konjac polysaccharide combining high fat groups. The mice were feed for twenty days. The levels of serum leptin were determined by ELISA method, and intestinal mucosa Na+ -K+-ATPase activities were determined by spectrophotometry. The body weight, adipose tissue and blood glucose were also examined. RESULTS At the 10th day, body weight and postprandial blood glucose of high-fat control group were (31.3 +/- 2.11) g and (7.5 +/- 1.15) mmol/L, those of high dose konjac polysaccharide combining high fat group were(28.0 +/- 2.06) g and (4.8 +/- 0.73) mmol/L. At the 20th day, concentrations of serum leptin and Na+ -K-ATPase activities of high-fat control group were (1078.5 +/- 61.69) pg/ml and (16.2 +/- 1.48) micromol Pi/(mg pro x h), those of high dose konjac polysaccharide combining high fat group were (820.5 +/- 58.52) pg/ml and (11.2 +/- 1.10) micromol Pi/(mg pro x h). All differences between two groups were significant (P < 0.05). CONCLUSION Konjac polysaccharide could decrease level of body weight, postprandial blood glucose, serum leptin and intestinal mucosa Na -K+-ATPase activity of mice treated with high fat.