Azotobacter vinelandii의 알긴산에 관한 연구
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investigation between the of Azotobacter vinelandii ІМV and phosphate-mobilizing bacteria Bacillus subtilis IMV B-7023 with nanoparticles of natural clay mineral – bentonite, on the viability of cells. Methods. Nitrogen-fixing bacteria A. vinelandii IMV B-7076 were cultivated in the Ashby medium, and phosphate-mobilizing bacteria B. subtilis IMV B-7023 – in glucose-mineral medium. In manufactured 10–20 % nanocomposite of bentonite 10% suspension of pure culture or the mixed bacterial culture were added. The viability of strains was determined after keeping them at room tempera-ture during 1 – 6 мonths. Electronic microscopy of bacteria was conducted with the trans-mis sion electronic microscope JEM-1400 Japan). Results. The interaction between A. vinelandii ІМV B-7076 and B. subtilis ІМV В-7023 –components of a complex bacterial preparation and nanoparticles of 10–20 % nanocomposite of bentonite enhanced the viability of these bacteria considerably. After six months of keeping them in 20 % bento nite, the number of viable Azotobacter cells decreased by 45 %, and of bacilli – by 11.5 %, whereas in the medium without nanomaterials – by over 2 orders and 2.2 times respec tively. The inoculation of wheat seeds with the complex nanocomposite bacterial prepa ration led to a considerable prolongation of the length of sprouts. Conclusions. The interaction between A. vinelandii ІМV В-7076 and B. subtilis ІМV В-7023 and nano-ben tonite enhanced the viability of these strains considerably, which is a prerequisite for manu fac turing the complex nanocomposite bacterial preparation for plant growing using these bacteria.
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