Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor.

Basic and acidic fibroblast growth factors (FGF's) are potent mitogens for capillary endothelial cells in vitro, stimulate angiogenesis in vivo, and may participate in tissue repair. An oligonucleotide probe for bovine basic FGF was designed from the nucleotide sequence of the amino-terminal exon of bovine acidic FGF, taking into account the 55 percent amino acid sequence homology between the two factors. With this oligonucleotide probe, a full length complementary DNA for basic FGF was isolated from bovine pituitary. Basic FGF in bovine hypothalamus was shown to be encoded by a single 5.0-kilobase messenger RNA; in a human hepatoma cell line, both 4.6- and 2.2-kilobase basic FGF messenger RNA's were present. Both growth factors seem to be synthesized with short amino-terminal extensions that are not found on the isolated forms for which the amino acid sequences have been determined. Neither basic nor acidic FGF has a classic signal peptide.

[1]  M. Klagsbrun,et al.  Purification and characterization of heparin-binding endothelial cell growth factors. , 1986, The Journal of biological chemistry.

[2]  R. Guillemin,et al.  Retina- and eye-derived endothelial cell growth factors: partial molecular characterization and identity with acidic and basic fibroblast growth factors. , 1985, Biochemistry.

[3]  G. Giménez-Gallego,et al.  Brain-derived acidic fibroblast growth factor: complete amino acid sequence and homologies. , 1985, Science.

[4]  N. Ling,et al.  Primary structure of bovine brain acidic fibroblast growth factor (FGF). , 1985, Biochemical and biophysical research communications.

[5]  G. Giménez-Gallego,et al.  Pure brain-derived acidic fibroblast growth factor is a potent angiogenic vascular endothelial cell mitogen with sequence homology to interleukin 1. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[6]  Y. Courtois,et al.  A unique family of endothelial cell polypeptide mitogens: the antigenic and receptor cross-reactivity of bovine endothelial cell growth factor, brain-derived acidic fibroblast growth factor, and eye-derived growth factor-II , 1985, The Journal of cell biology.

[7]  N. Ling,et al.  Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[8]  R. Lobb,et al.  Induction of angiogenesis by bovine brain derived class 1 heparin-binding growth factor. , 1985, Biochemistry.

[9]  Anita B. Roberts,et al.  Human transforming growth factor-β complementary DNA sequence and expression in normal and transformed cells , 1985, Nature.

[10]  S. Orkin,et al.  Cultured human endothelial cells express platelet-derived growth factor B chain: cDNA cloning and structural analysis , 1985, Nature.

[11]  C. March,et al.  Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs , 1985, Nature.

[12]  P. Pigeon,et al.  Immunoreactive fibroblast growth factor (FGF) in rat tissues: molecular weight forms and the effects of hypophysectomy. , 1985, Biochemical and biophysical research communications.

[13]  W I Wood,et al.  Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[14]  M. Klagsbrun,et al.  Purification of cartilage-derived growth factor by heparin affinity chromatography. , 1985, The Journal of biological chemistry.

[15]  M. Klagsbrun,et al.  Heparin affinity of anionic and cationic capillary endothelial cell growth factors: analysis of hypothalamus-derived growth factors and fibroblast growth factors. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[16]  P. Morméde,et al.  Immunoreactive fibroblast growth factor in cells of peritoneal exudate suggests its identity with macrophage-derived growth factor. , 1985, Biochemical and biophysical research communications.

[17]  R. Lobb,et al.  Purification of two distinct growth factors from bovine neural tissue by heparin affinity chromatography. , 1984, Biochemistry.

[18]  B. Keyt,et al.  Expression of active human factor VIII from recombinant DNA clones , 1984, Nature.

[19]  M. Klagsbrun,et al.  Endothelial cell mitogens derived from retina and hypothalamus: biochemical and biological similarities , 1984, The Journal of cell biology.

[20]  A. Schreiber,et al.  Heparin binds endothelial cell growth factor, the principal endothelial cell mitogen in bovine brain. , 1984, Science.

[21]  E. Chen,et al.  Human transforming growth factor-α: Precursor structure and expression in E. coli , 1984, Cell.

[22]  J. Folkman,et al.  Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor. , 1984, Science.

[23]  M. Kozak.  Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. , 1984, Nucleic acids research.

[24]  S. Anderson,et al.  Isolation of a genomic clone for bovine pancreatic trypsin inhibitor by using a unique-sequence synthetic DNA probe. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[25]  P. Leder,et al.  The human c-myc oncogene: Structural consequences of translocation into the igh locus in Burkitt lymphoma , 1983, Cell.

[26]  Gunnar von Heijne,et al.  Patterns of Amino Acids near Signal‐Sequence Cleavage Sites , 1983 .

[27]  M. Jaye,et al.  Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX. , 1983, Nucleic acids research.

[28]  S. Camper,et al.  Cloning and nucleotide sequencing of the bovine growth hormone gene. , 1982, Nucleic acids research.

[29]  J. Vieira,et al.  A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. , 1982, Gene.

[30]  D. Gospodarowicz,et al.  The angiogenic activity of the fibroblast and epidermal growth factor. , 1979, Experimental eye research.

[31]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[32]  H. Boedtker,et al.  RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. , 1977, Biochemistry.

[33]  A. Ullrich,et al.  Insulin-like growth factor II precursor gene organization in relation to insulin gene family , 1984, Nature.