Robust high-yield methodologies for (2)H and (2)H/(15)N/(13)C labeling of proteins for structural investigations using neutron scattering and NMR.
暂无分享,去创建一个
A. Duff | A. Rekas | K. Wilde | Agata Rekas | Anthony P Duff | Karyn L Wilde | Vanessa Lake | Peter J Holden | P. Holden | Vanessa Lake
[1] B. Farmer,et al. High-level 2H/13C/15N labeling of proteins for NMR studies , 1995, Journal of biomolecular NMR.
[2] T. Gunasekera,et al. Growth of E. coli BL21 in minimal media with different gluconeogenic carbon sources and salt contents , 2006, Applied Microbiology and Biotechnology.
[3] J. Trewhella,et al. Troponin I encompasses an extended troponin C in the Ca(2+)-bound complex: a small-angle X-ray and neutron scattering study. , 1994, Biochemistry.
[4] I. Schlichting,et al. Production and X-ray crystallographic analysis of fully deuterated cytochrome P450cam. , 2005, Acta crystallographica. Section D, Biological crystallography.
[5] B. Leiting,et al. Predictable deuteration of recombinant proteins expressed in Escherichia coli. , 1998, Analytical biochemistry.
[6] P. Moore. The preparation of deuterated ribosomal materials for neutron scattering. , 1979, Methods in enzymology.
[7] D. James,et al. Low-resolution solution structures of Munc18:Syntaxin protein complexes indicate an open binding mode driven by the Syntaxin N-peptide , 2012, Proceedings of the National Academy of Sciences.
[8] J. Trewhella,et al. Calmodulin binds a highly extended HIV-1 MA protein that refolds upon its release. , 2012, Biophysical journal.
[9] D. Brockwell,et al. Improved methods of cultivation and production of deuteriated proteins from E. coli strains grown on fully deuteriated minimal medium. , 2003, Journal of applied microbiology.
[10] K. Nierhaus,et al. Large-scale preparation of fully deuterated cell components. Ribosomes from Escherichia coli with high biological activity. , 1993, European journal of biochemistry.
[11] D. LeMaster. Isotope labeling in solution protein assignment and structural analysis , 1994 .
[12] A. Goldman,et al. Production and preliminary analysis of perdeuterated yeast inorganic pyrophosphatase crystals suitable for neutron diffraction. , 2004, Acta crystallographica. Section D, Biological crystallography.
[13] L. Kay,et al. The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins. , 1998, Annual review of biophysics and biomolecular structure.
[14] F. Studier,et al. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. , 1986, Journal of molecular biology.
[15] F. Studier,et al. Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. , 1991, Journal of molecular biology.
[16] A. Middelberg,et al. High yield expression and efficient purification of deuterated human protein galectin-2 , 2012 .
[17] A. van Dorsselaer,et al. High-resolution neutron protein crystallography with radically small crystal volumes: application of perdeuteration to human aldose reductase. , 2005, Acta crystallographica. Section D, Biological crystallography.
[18] M. Paabo,et al. Use of the glass electrode in deuterium oxide and the relation between the standardized pD (paD) scale and the operational pH in heavy water , 1968 .
[19] Clay Bracken,et al. A method for efficient isotopic labeling of recombinant proteins , 2001, Journal of biomolecular NMR.
[20] E. Anderson. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B". , 1946, Proceedings of the National Academy of Sciences of the United States of America.
[21] A. Bax,et al. Staphylococcal nuclease: sequential assignments and solution structure. , 1989, Biochemistry.
[22] Anton P. J. Middelberg,et al. A novel technique for the measurement of disruption in high‐pressure homogenization: Studies on E. coli containing recombinant inclusion bodies , 1991, Biotechnology and bioengineering.
[23] A. Kahru,et al. Glucose-limited fed-batch cultivation of Escherichia coli with computer-controlled fixed growth rate. , 1990, Biotechnology and bioengineering.
[24] M. Sunde,et al. Solid-state NMR spectroscopy of functional amyloid from a fungal hydrophobin: a well-ordered β-sheet core amidst structural heterogeneity. , 2012, Angewandte Chemie.
[25] B P Schoenborn,et al. Enhanced visibility of hydrogen atoms by neutron crystallography on fully deuterated myoglobin. , 2000, Proceedings of the National Academy of Sciences of the United States of America.
[26] F. Richards,et al. Preparative-scale isolation of isotopically labeled amino acids. , 1982, Analytical biochemistry.
[27] E. M. Barnes,et al. Feedback inhibition of ammonium (methylammonium) ion transport in Escherichia coli by glutamine and glutamine analogs , 1987, Journal of bacteriology.
[28] D. Mustafi,et al. Overexpression and biosynthetic deuterium enrichment of TEM-1 beta-lactamase for structural characterization by magnetic resonance methods. , 2000, Protein expression and purification.
[29] G M Clore,et al. An efficient and cost-effective isotope labeling protocol for proteins expressed in shape Escherichia coli , 1998, Journal of biomolecular NMR.
[30] I. Booth,et al. Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity. , 2002, Microbiology.
[31] D. Myles,et al. Structural stability and dynamics of hydrogenated and perdeuterated cytochrome P450cam (CYP101). , 2004, Biochemistry.
[32] Peter F. Stanbury,et al. Principles of Fermentation Technology , 1984 .
[33] Victoria L. Murray,et al. Practical protocols for production of very high yields of recombinant proteins using Escherichia coli , 2009, Protein science : a publication of the Protein Society.
[34] S. Enfors,et al. Modeling of Overflow Metabolism in Batch and Fed‐Batch Cultures of Escherichiacoli , 1999, Biotechnology progress.
[35] K. Clauser,et al. The production and X-ray structure determination of perdeuterated Staphylococcal nuclease. , 1994, Biophysical chemistry.
[36] A. Middelberg,et al. The effects of acid hydrolysis on protein biosurfactant molecular, interfacial, and foam properties: pH responsive protein hydrolysates , 2012 .
[37] F. Baneyx. Recombinant protein expression in Escherichia coli. , 1999, Current opinion in biotechnology.