Increased expression of the HDJ-2 heat shock protein in biopsies of human rejected kidney.

The presence of donor-specific alloreactive helper and cytotoxic T cells has been described in allograft biopsies obtained from individuals undergoing acute allograft rejection of various solid organs. However, not all of these lymphocytes demonstrated specificity to mismatched donor HLA antigens. The identity of the antigens to which these T cells are directed to is still unknown at present. The possibility that heat shock proteins (Hsp) could serve as antigenic determinants to which these T cells respond has been raised. We have recently cloned and characterized a novel Hsp of 45Kd molecular weight. In the present study we show that the synthesis of this Hsp (HDJ-2) as well as Hsp60 is significantly elevated in kidney biopsies from individuals undergoing acute and chronic rejection. No message was detected either for HDJ-2 or Hsp60 in biopsies obtained from normal pretransplant kidneys or posttransplant kidneys with no rejection. However, there was some increase in Hsp in miscellaneous causes of allograft dysfunction such as infection and drug allergy. But, this was not as consistent as that noted for allograft rejection. This marked increase in Hsp expression during allograft rejection suggests Hsps as potential candidates for antigenic determinants contributing to kidney rejection.

[1]  B. Griffith,et al.  Heat shock protein-induced T-lymphocyte propagation from endomyocardial biopsies in heart transplantation. , 1995, The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation.

[2]  M. Ryan,et al.  Role of chaperones in the biogenesis and maintenance of the mitochondrion , 1995, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[3]  F. Pan,et al.  Heat shock protein reactivity of lymphocytes isolated from heterotopic rat cardiac allografts. , 1995, Transplantation.

[4]  M. Cheetham,et al.  Regulation of 70-kDa heat-shock-protein ATPase activity and substrate binding by human DnaJ-like proteins, HSJ1a and HSJ1b. , 1994, European journal of biochemistry.

[5]  I. Benjamin.  Stress proteins: Is their application in clinical medicine on the horizon? , 1993, Hepatology.

[6]  P. Srivastava,et al.  Heat shock protein 70-associated peptides elicit specific cancer immunity , 1993, The Journal of experimental medicine.

[7]  T. Mohanakumar,et al.  Cloning of a unique human homologue of the Escherichia coli DNAJ heat shock protein. , 1993, Biochimica et biophysica acta.

[8]  D. Cyr,et al.  Eukaryotic homologues of Escherichia coli dnaJ: a diverse protein family that functions with hsp70 stress proteins. , 1993, Molecular biology of the cell.

[9]  L. Bow,et al.  Heat shock and recovery protects renal allografts from warm ischemic injury and enhances HSP72 production. , 1993, Transplantation proceedings.

[10]  J. Pleasure,et al.  A mechanism of glycine and alanine cytoprotective action: stimulation of stress-induced HSP70 mRNA. , 1992, Kidney international.

[11]  H. Itoh,et al.  Renal localization of the constitutive 73-kDa heat-shock protein in normal and PAN rats. , 1992, Kidney international.

[12]  H. Itoh,et al.  Localization of 90-kDa heat shock protein in the kidney. , 1990, Kidney international.

[13]  M. Yacoub,et al.  Selection for donor-specific cytotoxic T lymphocytes within the allografted human heart. , 1990, Transplantation.

[14]  R. Currie.  Effects of ischemia and perfusion temperature on the synthesis of stress-induced (heat shock) proteins in isolated and perfused rat hearts. , 1987, Journal of molecular and cellular cardiology.

[15]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[16]  C. Lovis,et al.  Heat shock proteins and the kidney. , 1994, Renal failure.

[17]  M. Harada,et al.  Autoreactive and heat shock protein 60-recognizing CD4+ T-cells show antitumor activity against syngeneic fibrosarcoma. , 1993, Cancer research.