Determination of ochratoxin A in pig liver-derived pa?te´s by high-performance liquid chromatography

A solid phase extraction procedure followed by HPLC analysis with fluorescence detection has been developed for ochratoxin A (OTA) in pâtés derived from pig liver. After a previous extraction of OTA with 60% acetonitrile, all the samples were purified through C8 columns. The percentage recovery was 85.7% and the lower limits calculated for accurate detection and quantitation were 0.56ng/g and 0.84ng/g respectively. The HPLC procedure showed a good linearity in the interval of equivalent OTA concentrations of 0.84 to 3ng/g. Values <10% were obtained for precision in HPLC determinations performed (n=3) and (n=9). Stability of calibration standards and samples during the analytical procedure was also demonstrated. This method was successfully applied to 38 pig-derived pâtés and three samples were found to be positive with OTA levels above the detection limit. The highest concentration (1.77ng/g) has been found in a home-made pâté.

[1]  E. González-Peñas,et al.  A high-performance liquid-chromatographic method for the determination of ochratoxin a in human plasma , 1999 .

[2]  H Valenta,et al.  Chromatographic methods for the determination of ochratoxin A in animal and human tissues and fluids. , 1998, Journal of chromatography. A.

[3]  K. Jørgensen.  Survey of pork, poultry, coffee, beer and pulses for ochratoxin A. , 1998, Food additives and contaminants.

[4]  E. González-Peñas,et al.  Exposure to Ochratoxin a in Europe: Comparison with a Region of Northern Spain , 1998 .

[5]  A. Williams,et al.  An intercomparison of methods for the determination of ochratoxin A in pig kidney. , 1997, Food additives and contaminants.

[6]  R. Causon.  Validation of chromatographic methods in biomedical analysis. Viewpoint and discussion. , 1997, Journal of chromatography. B, Biomedical sciences and applications.

[7]  H. Steinhart,et al.  Can food quality be enhanced by chromatography? , 1997 .

[8]  M. Goll,et al.  Determination of ochratoxin A in regional samples of cow's milk from Germany. , 1996, Food additives and contaminants.

[9]  A. De Dominicis,et al.  Ochratoxin A levels in human milk and related food samples: an exposure assessment. , 1995, Natural toxins.

[10]  H. P. Egmond.  Mycotoxins: Regulations, quality assurance and reference materials , 1995 .

[11]  B Zimmerli,et al.  Determination of ochratoxin A at the ppt level in human blood, serum, milk and some foodstuffs by high-performance liquid chromatography with enhanced fluorescence detection and immunoaffinity column cleanup: methodology and Swiss data. , 1995, Journal of chromatography. B, Biomedical applications.

[12]  H. V. van Egmond.  Mycotoxins: regulations, quality assurance and reference materials. , 1995, Food additives and contaminants.

[13]  J. Miller,et al.  Mycotoxins in grain: compounds other than aflatoxin. , 1994 .

[14]  J. Miller,et al.  Penicillium and Aspergillus toxins. , 1994 .

[15]  K. Hult,et al.  Plasma ochratoxin A levels in three Swedish populations surveyed using an ion-pair HPLC technique. , 1991, Food additives and contaminants.

[16]  B. Hald.  Ochratoxin A in human blood in European countries. , 1991, IARC scientific publications.

[17]  K. Hult,et al.  Toxicokinetics of ochratoxin A in several species and its plasma‐binding properties , 1989, Journal of applied toxicology : JAT.

[18]  G. Dirheimer,et al.  Monitoring and identification of fungal toxins in food products, animal feed and cereals in Tunisia , 1988 .

[19]  M. Gareis,et al.  [Determination and occurrence of ochratoxin A in slaughtered swine]. , 1984, Berliner und Munchener tierarztliche Wochenschrift.

[20]  K. Hult,et al.  Mycotoxic porcine nephropathy and spontaneous occurrence of ochratoxin A residues in kidneys and blood of Polish swine , 1984, Applied and environmental microbiology.

[21]  B. Josefsson,et al.  Heat stability of ochratoxin A in pig products. , 1980, Journal of the science of food and agriculture.

[22]  K. Hult,et al.  Ochratoxin A as the cause of spontaneous nephropathy in fattening pigs , 1978, Applied and environmental microbiology.

[23]  W Fritz,et al.  [Determination of ochratoxin A]. , 1974, Die Nahrung.