Quantum dots pushing up in vitro diagnostics limits

Biopsies are conventionally performed in two dimensions. Histological slices in general present some micrometers in thickness, allowing that some molecular domains stay out of the resulting image. Thus the histopathological assay potentially is based on an incomplete set of information. The use of quantum dots as fluorescente probes allows the investigation of labelling pattern and biomarkers expression, along the three-dimensions of fresh histological slices, leading to more precise results. Present work show and discuss pattern and fluorescence intensity emission at the visible region obtained as a function of tissue thickness in histological (thickness(z)=7.6μm) breast cancer samples labeled with compact (7-10 nm) water soluble quantum dots. Series of 154 three-dimensional (3D) images were recorded from each tissue sample by laser scanning confocal microscopy, using 488 nm excitation.. In order to compare the results obtained, all the acquisition parameters were maintained constant. Results point to the possibility of more accurate histological diagnostics, once they clearly show distinct labeling patterns across sample thickness.

[1]  L. Chung,et al.  Quantum dots-based multiplexed immunohistochemistry of protein expression in human prostate cancer cells. , 2009, European journal of histochemistry : EJH.

[2]  L. True.  Quality control in molecular immunohistochemistry , 2008, Histochemistry and Cell Biology.

[3]  M. Nadji,et al.  Tissue localization of therapy targets in cancer: Immunohistochemistry and beyond , 2008 .

[4]  David L. Schwartz,et al.  Imaging Epidermal Growth Factor Receptor Expression In vivo: Pharmacokinetic and Biodistribution Characterization of a Bioconjugated Quantum Dot Nanoprobe , 2008, Clinical Cancer Research.

[5]  D. Rimm.  What brown cannot do for you , 2006, Nature Biotechnology.

[6]  E. Tholouli,et al.  Quantum dots light up pathology , 2008, The Journal of pathology.

[7]  Hassan M E Azzazy,et al.  In vitro diagnostic prospects of nanoparticles. , 2009, Clinica chimica acta; international journal of clinical chemistry.

[8]  Igor Nabiev,et al.  Fluorescent nanocrystal quantum dots as medical diagnostic tools. , 2008, Expert opinion on medical diagnostics.

[9]  Arezou A Ghazani,et al.  High throughput quantification of protein expression of cancer antigens in tissue microarray using quantum dot nanocrystals. , 2006, Nano letters.

[10]  Rosario Pereiro,et al.  Bioanalytics and biolabeling with semiconductor nanoparticles (quantum dots) , 2007 .

[11]  L. True,et al.  Quantum dots for molecular pathology: their time has arrived. , 2007, The Journal of molecular diagnostics : JMD.

[12]  T. Deerinck.  The Application of Fluorescent Quantum Dots to Confocal, Multiphoton, and Electron Microscopic Imaging , 2008, Toxicologic pathology.

[13]  A. Fontes,et al.  Quantum Dots: Applications in Biology , 2014, Methods in Molecular Biology.

[14]  J. Rao,et al.  Fluorescence imaging in vivo: recent advances. , 2007, Current opinion in biotechnology.

[15]  Igor L. Medintz,et al.  Maltose-binding protein: a versatile platform for prototyping biosensing. , 2006, Current opinion in biotechnology.

[16]  Igor Nabiev,et al.  Highly Stable Fluorescent Nanocrystals as a Novel Class of Labels for Immunohistochemical Analysis of Paraffin-Embedded Tissue Sections , 2002, Laboratory Investigation.

[17]  Beate S. Santos,et al.  Semiconductor Quantum Dots for Biological Applications , 2008 .

[18]  C Womack,et al.  Quantitative multiplexed quantum dot immunohistochemistry. , 2008, Biochemical and biophysical research communications.

[19]  A. Fontes,et al.  Core‐shell CdS/Cd(OH)2 quantum dots: synthesis and bioconjugation to target red cells antigens , 2005, Journal of microscopy.

[20]  Quantum dots as fluorescent bio‐labels in cancer diagnostic , 2006 .

[21]  Beate S. Santos,et al.  CdS-Cd(OH)2 core shell quantum dots functionalized with Concanavalin A lectin for recognition of mammary tumors , 2006 .

[22]  I. Kanno,et al.  Silica-shelled single quantum dot micelles as imaging probes with dual or multimodality. , 2006, Analytical chemistry.

[23]  J. Käs,et al.  Quantum dots – a versatile tool in plant science? , 2006, Journal of nanobiotechnology.

[24]  Youngho Seo,et al.  Nanoprobes for Medical Diagnosis: Current Status of Nanotechnology in Molecular Imaging , 2008 .

[25]  Shuming Nie,et al.  In Situ Molecular Profiling of Breast Cancer Biomarkers with Multicolor Quantum Dots , 2007 .

[26]  K. Jain,et al.  Applications of nanobiotechnology in clinical diagnostics. , 2007, Clinical chemistry.

[27]  W. Fraser Symmans,et al.  Standardizing Slide-Based Assays in Breast Cancer: Hormone Receptors, HER2, and Sentinel Lymph Nodes , 2007, Clinical Cancer Research.

[28]  Clive R Taylor,et al.  Recommendations for Improved Standardization of Immunohistochemistry , 2007, Applied immunohistochemistry & molecular morphology : AIMM.

[29]  R. Nitschke,et al.  Quantum dots versus organic dyes as fluorescent labels , 2008, Nature Methods.

[30]  J. Itoh,et al.  Quantum dots for multicolor tumor pathology and multispectral imaging. , 2007, Methods in molecular biology.

[31]  Colloidal semiconductor quantum dots : Potential tools for new diagnostic methods , 2008 .