Expression of neural cell adhesion molecule (N-CAM) in rat islets and its role in islet cell type segregation.

Endocrine cell types are non-randomly distributed within pancreatic islets of Langerhans. In the rat, insulin-secreting B-cells occupy the core of the islets and are surrounded by A-, D- and PP-cells, secreting glucagon, somatostatin and pancreatic polypeptide, respectively. Furthermore, dissociated islet cells have the ability in vitro to form aggregates with the same cell-type organization as native islets (pseudoislets). These observations suggest that a differential expression of cell adhesion molecules (CAMs) might characterize B- and non-B-cells (A-, D- and PP-cells), and be in part responsible for the establishment and maintenance of islet architecture. Indirect immunofluorescence using antibodies against CAMs and islet hormones was performed on serial sections of the splenic and duodenal parts of the rat pancreas. Staining for the Ca(2+)-dependent CAM E-cadherin was detected on both exocrine and endocrine tissue and was uniform over the entire islet section, in both pancreatic regions. By contrast, staining for the Ca(2+)-independent neural CAM (N-CAM) was restricted to endocrine tissue and nerve endings. Furthermore, N-CAM staining of endocrine cells was stronger in the islet periphery, a region composed mostly of non-B-cells. Serial sections demonstrate that cells staining strongly for N-CAM in the splenic part correspond to glucagon cells and in the duodenal part to pancreatic polypeptide cells. Within pseudoislets in vitro a stronger staining for N-CAM was also observed on peripheral cells, corresponding to non-B-cells.

[1]  D. Hanahan,et al.  Molecular cloning of mouse pancreatic islet R-cadherin: differential expression in endocrine and exocrine tissue. , 1993, Molecular endocrinology.

[2]  P. Halban,et al.  Tumor necrosis factor-alpha modifies adhesion properties of rat islet B cells. , 1993, The Journal of clinical investigation.

[3]  J. Lilien,et al.  Cadherin-mediated adhesion in pancreatic islet cells is modulated by a cell surface N-acetylgalactosaminylphosphotransferase. , 1992, Journal of cell science.

[4]  S. Baekkeskov,et al.  Differential expression of neural cell adhesion molecule and cadherins in pancreatic islets, glucagonomas, and insulinomas. , 1992, Molecular endocrinology.

[5]  L. Orci,et al.  Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene. , 1991, Development.

[6]  P. Halban,et al.  Uvomorulin mediates calcium-dependent aggregation of islet cells, whereas calcium-independent cell adhesion molecules distinguish between islet cell types. , 1991, Developmental biology.

[7]  C. Lardinois,et al.  A morphological and immunohistochemical investigation of endocrine pancreata from obese ob+/ob+ mice. , 1991, Acta histochemica.

[8]  P. Halban,et al.  Differences in aggregation properties and levels of the neural cell adhesion molecule (NCAM) between islet cell types. , 1990, Experimental cell research.

[9]  G. Edelman,et al.  Expression of chicken liver cell adhesion molecule fusion genes in transgenic mice. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[10]  J. Gagliardino,et al.  Sequential Morphological Changes in Pancreatic Islets of Spontaneously Diabetic Rats , 1990, Pancreas.

[11]  U. Rutishauser.  Neural cell adhesion molecule as a regulator of cell-cell interactions. , 1990, Advances in experimental medicine and biology.

[12]  G. Edelman,et al.  Cell sorting-out is modulated by both the specificity and amount of different cell adhesion molecules (CAMs) expressed on cell surfaces. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[13]  C. Damsky,et al.  Expression of the cell-cell adhesion glycoprotein cell-CAM 120/80 in normal human tissues and tumors. , 1989, The American journal of pathology.

[14]  O. K. Langley,et al.  Expression of the neural cell adhesion molecule NCAM in endocrine cells. , 1989, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[15]  P. Armstrong,et al.  Cell sorting out: the self-assembly of tissues in vitro. , 1989, Critical reviews in biochemistry and molecular biology.

[16]  Akinao Nose,et al.  Expressed recombinant cadherins mediate cell sorting in model systems , 1988, Cell.

[17]  Philippe A. Halban,et al.  Altered differentiated cell surface properties of transformed (RINm5F) compared with native adult rat pancreatic B cells. , 1988, Endocrinology.

[18]  J. Sunshine,et al.  The neural cell adhesion molecule (NCAM) as a regulator of cell-cell interactions. , 1988, Science.

[19]  S. Bonner-Weir,et al.  Spontaneous Reassociation of Dispersed Adult Rat Pancreatic Islet Cells Into Aggregates With Three-Dimensional Architecture Typical of Native Islets , 1987, Diabetes.

[20]  A. Nose,et al.  A novel cadherin cell adhesion molecule: its expression patterns associated with implantation and organogenesis of mouse embryos , 1986, The Journal of cell biology.

[21]  P. Morris,et al.  ISOLATION OF RAT PANCREATIC ISLETS BY DUCTAL INJECTION OF COLLAGENASE1 , 1986, Transplantation.

[22]  M. Takeichi,et al.  Expression of N-cadherin adhesion molecules associated with early morphogenetic events in chick development , 1986, Nature.

[23]  G M Edelman,et al.  Cell adhesion molecules in the regulation of animal form and tissue pattern. , 1986, Annual review of cell biology.

[24]  U. Rutishauser.  Developmental biology of a neural cell adhesion molecule , 1984, Nature.

[25]  M. Takeichi,et al.  Molecular nature of the calcium-dependent cell-cell adhesion system in mouse teratocarcinoma and embryonic cells studied with a monoclonal antibody. , 1984, Developmental biology.

[26]  L. Orci,et al.  Collagen matrix promotes reorganization of pancreatic endocrine cell monolayers into islet-like organoids , 1983, The Journal of cell biology.

[27]  G. Edelman,et al.  Early epochal maps of two different cell adhesion molecules. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[28]  G. Edelman,et al.  Characterization of L-CAM, a major cell adhesion molecule from embryonic liver cells. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[29]  G. Edelman,et al.  Cell adhesion molecules in early chicken embryogenesis. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[30]  J. Tai,et al.  Preparation of pseudoislets for morphological and functional studies. , 1982, Transplantation.

[31]  D. Pipeleers,et al.  Islet cell analysis and purification by light scatter and autofluorescence. , 1982, Biochemical and biophysical research communications.

[32]  F. Jacob,et al.  Cell-cell interactions in early embryogenesis: A molecular approach to the role of calcium , 1981, Cell.

[33]  L. Orci,et al.  Glucagon and the A cell: physiology and pathophysiology (second of two parts). , 1981, The New England journal of medicine.

[34]  F. Jacob,et al.  A cell surface glycoprotein involved in the compaction of embryonal carcinoma cells and cleavage stage embryos , 1980, Cell.

[35]  L. Orci,et al.  Alteration of Islet Cell Populations in Spontaneously Diabetic Mice , 1978, Diabetes.

[36]  L Orci,et al.  Insulin, glucagon, and somatostatin secretion in the regulation of metabolism. , 1978, Annual review of physiology.

[37]  L. Orci,et al.  Glucagon and the A cells. , 2010, Recent progress in hormone research.

[38]  L Orci,et al.  Pancreatic polypeptide and glucagon : non-random distribution in pancreatic islets. , 1976, Life sciences.

[39]  L. Orci,et al.  Hypertrophy and hyperplasia of somatostatin-containing D-cells in diabetes. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[40]  L. Orci,et al.  FUNCTIONAL SUBDIVISION OF ISLETS OF LANGERHANS AND POSSIBLE ROLE OF D CELLS , 1975, The Lancet.

[41]  L. Herzenberg,et al.  ANALYSIS OF CELL POPULATIONS WITH A FLUORESCENCE‐ACTIVATED CELL SORTER * , 1975, Annals of the New York Academy of Sciences.

[42]  Malcolm S. Steinberg,et al.  Reconstruction of Tissues by Dissociated Cells , 1963 .

[43]  H. V. Wilson.  On some phenomena of coalescence and regeneration in sponges , 1907 .