Highly selective recognition and fluorescent detection of JEV via virus-imprinted magnetic silicon microspheres

Abstract A novel virus-recognition fluorescent sensor based on virus-imprinted polymers (VIP) for highly selective and highly sensitive detection of Japanese encephalitis virus (JEV) was presented. The virus-imprinted method went beyond simple shape imprinting through surface imprinting with magnetic silicon microspheres acting as carrier materials and tetraethyl orthosilicate (TEOS) functioning as building blocks. The resulting VIP film could selectively capture target JEV via recognition cavities, and a high imprinting factor of 2.98 was achieved. Furthermore, fast magnetic separation and highly sensitive fluorescent detection in water were offered for JEV, showing a good linearity within 2.5–45 nM (R2 = 0.9948). Finally, the specificity study confirmed that the virus-imprinted magnetic silicon microspheres demonstrated excellent selectivity for JEV in several different types of virus.

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