Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling.

Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.

[1]  Artur Makarewicz,et al.  Electronic Supplementary Material ( ESI ) for ChemComm , 2015 .

[2]  P. Orság,et al.  GFP-like fluorophores as DNA labels for studying DNA-protein interactions. , 2012, The Journal of organic chemistry.

[3]  M. Fojta,et al.  Synthesis of Hydrazone‐Modified Nucleotides and Their Polymerase Incorporation onto DNA for Redox Labeling , 2012 .

[4]  P. Orság,et al.  Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA–protein interactions , 2012 .

[5]  Michal Hocek,et al.  Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction. , 2012, Chemical communications.

[6]  R. Pohl,et al.  Synthesis of aldehyde-linked nucleotides and DNA and their bioconjugations with lysine and peptides through reductive amination. , 2012, Chemistry.

[7]  R. Pohl,et al.  Synthesis and photophysical properties of biaryl-substituted nucleos(t)ides. Polymerase synthesis of DNA probes bearing solvatochromic and pH-sensitive dual fluorescent and 19F NMR labels. , 2012, The Journal of organic chemistry.

[8]  A. Marx,et al.  Barcoded nucleotides. , 2012, Angewandte Chemie.

[9]  M. Fojta,et al.  Anthraquinone as a redox label for DNA: synthesis, enzymatic incorporation, and electrochemistry of anthraquinone-modified nucleosides, nucleotides, and DNA. , 2011, Chemistry.

[10]  R. Pohl,et al.  Transient and switchable (triethylsilyl)ethynyl protection of DNA against cleavage by restriction endonucleases. , 2011, Angewandte Chemie.

[11]  M. Fojta,et al.  Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl transferase. Application in electrochemical DNA hybridization and protein-DNA binding assays. , 2011, Organic & biomolecular chemistry.

[12]  R. Pohl,et al.  Cleavage of Functionalized DNA Containing 5‐Modified Pyrimidines by Type II Restriction Endonucleases , 2011, Chembiochem : a European journal of chemical biology.

[13]  R. Pohl,et al.  Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines. , 2010, Angewandte Chemie.

[14]  Michal Hocek,et al.  Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases , 2009, Nucleic acids research.

[15]  S. Howorka,et al.  Synthesis and enzymatic incorporation of modified deoxyuridine triphosphates. , 2009, Organic & biomolecular chemistry.

[16]  M. Fojta,et al.  Base-modified DNA labeled by [Ru(bpy)(3)](2+) and [Os(bpy)(3)](2+) complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications. , 2009, Chemistry.

[17]  T. Carell,et al.  Controlled nucleation of DNA metallization. , 2009, Angewandte Chemie.

[18]  G. Jeschke,et al.  Enzymatic synthesis of multiple spin-labeled DNA. , 2008, Angewandte Chemie.

[19]  M. Fojta,et al.  Cross-coupling reactions of nucleoside triphosphates followed by polymerase incorporation. Construction and applications of base-functionalized nucleic acids. , 2008, Organic & biomolecular chemistry.

[20]  M. Fojta,et al.  Aminophenyl- and nitrophenyl-labeled nucleoside triphosphates: synthesis, enzymatic incorporation, and electrochemical detection. , 2008, Angewandte Chemie.

[21]  M. Fojta,et al.  Ferrocenylethynyl derivatives of nucleoside triphosphates: synthesis, incorporation, electrochemistry, and bioanalytical applications. , 2007, Chemistry.

[22]  P. Čapek,et al.  An efficient method for the construction of functionalized DNA bearing amino acid groups through cross-coupling reactions of nucleoside triphosphates followed by primer extension or PCR. , 2007, Chemistry.

[23]  I-Ming Hsing,et al.  Electrochemical real-time polymerase chain reaction. , 2006, Journal of the American Chemical Society.

[24]  Michael Famulok,et al.  A versatile toolbox for variable DNA functionalization at high density. , 2005, Journal of the American Chemical Society.

[25]  J Justin Gooding,et al.  Enzymatic synthesis of redox-labeled RNA and dual-potential detection at DNA-modified electrodes. , 2004, Angewandte Chemie.

[26]  J Justin Gooding,et al.  Multipotential electrochemical detection of primer extension reactions on DNA self-assembled monolayers. , 2004, Journal of the American Chemical Society.

[27]  M. Metzker,et al.  Rigid, conjugated, fluoresceinated thymidine triphosphates: syntheses and polymerase mediated incorporation into DNA analogues. , 2003, Chemistry.

[28]  G. King,et al.  Ferrocene conjugates of dUTP for enzymatic redox labelling of DNA. , 2002, Nucleic acids research.

[29]  Y. Tor,et al.  Redox-active metal-containing nucleotides: synthesis, tunability, and enzymatic incorporation into DNA. , 2002, Journal of the American Chemical Society.

[30]  W. Ganzenmüller.  Ein italienisches Goldlüsterrezept vom Ende des 14. Jahrhunderts , 1938 .