Expression of a receptor protein tyrosine phosphatase in human glial tumors

We have analyzed expression of a receptor protein tyrosine phosphatase (RPTPζ/β) in tissue samples from 23 human gliomas. Using the reverse transcription-polymerase chain reaction (RT-PCR) technique, we assayed for the presence or absence of mRNA transcripts encoding the intact receptor and 2 alternatively spliced forms of RPTPζ/β. Transcripts encoding the intact and truncated receptors were expressed in all of the lower grade gliomas (WHO grade 1–3) analyzed, but not in 55% of the grade 4 glioblastomas multiforme (GBM). However, this subset of GBMs did express an alternatively spliced secreted form comprised of only the RPTPζ/β extracellular domain. Our data suggests there may be a correlation between the loss of transcripts encoding the receptor forms of RPTPζ/β and progression from low to high grade gliomas. This work provides additional evidence for the importance of phosphatase isoform expression in human tumors.

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