Current status of flow cytometry in cell and molecular biology.

This review summarizes recent developments in flow cytometry (FC). It gives an overview of techniques currently available, in terms of apparatus and sample handling, a guide to evaluating applications, an overview of dyes and staining methods, an introduction to internet resources, and a broad listing of classic references and reviews in various fields of interest, as well as some recent interesting articles.

[1]  M. Van Dilla,et al.  Flow microfluorometric studies of lectin binding to mammalian cells. I. general features , 1973, Journal of cellular physiology.

[2]  T. Strom,et al.  Multiparameter flow cytometric analysis of lymphocyte subpopulation activation in lectin-stimulated cultures. , 1982, Journal of immunology.

[3]  G. Frelat,et al.  Recent advances of flow cytometry in fundamental and applied microbiology , 1993, Biology of the cell.

[4]  J. Hindkjær,et al.  Ring chromosome 20 with loss of telomeric sequences detected by multicolour PRINS , 1993, Clinical genetics.

[5]  R. Kalejta,et al.  Simultaneous analysis of the cyan, yellow and green fluorescent proteins by flow cytometry using single-laser excitation at 458 nm. , 1999, Cytometry.

[6]  S. Burgess,et al.  Counting absolute numbers of specific leukocyte subpopulations in avian whole blood using a single-step flow cytometric technique: comparison of two inbred lines of chickens. , 1999, Journal of immunological methods.

[7]  J. Merino,et al.  Simultaneous evaluation of phagocytosis and Fc gamma R-mediated oxidative burst in human monocytes by a simple flow cytometry method. , 1993, Journal of immunological methods.

[8]  Z. Darżynkiewicz,et al.  Expression of G1 and G2 cyclins measured in individual cells by multiparameter flow cytometry: a new tool in the analysis of the cell cycle , 1994 .

[9]  J. Steinkamp,et al.  Phagocytosis: flow cytometric quantitation with fluorescent microspheres. , 1982, Science.

[10]  J. Russo,et al.  A Review of DNA Flow Cytometric Preparatory and Analytical Methods , 1985 .

[11]  A. Fattorossi,et al.  Flow cytometric analysis of the early phases of apoptosis by cellular and nuclear techniques. , 1996, Cytometry.

[12]  J. Hoeg,et al.  Filipin as a flow microfluorometry probe for cellular cholesterol. , 1984, Cytometry.

[13]  D. Shrieve,et al.  Quantitative analysis of cellular glutathione by flow cytometry utilizing monochlorobimane: some applications to radiation and drug resistance in vitro and in vivo. , 1986, Cancer research.

[14]  S. E. Petersen,et al.  A comparison between flow cytometric ploidy investigation and chromosome analysis of 32 human colorectal tumors. , 1986, Cytometry.

[15]  W. Kuo,et al.  Molecular cytometry applied to detection and characterization of disease-linked chromosome aberrations. , 1991, Bailliere's clinical haematology.

[16]  D. Hedley,et al.  Evaluation of methods for measuring cellular glutathione content using flow cytometry. , 1994, Cytometry.

[17]  J. Ringelberg,et al.  Optical plankton analyser: a flow cytometer for plankton analysis, I: Design considerations. , 1989, Cytometry.

[18]  D Marie,et al.  Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analysis of marine prokaryotes , 1996, Applied and environmental microbiology.

[19]  E. Hamori,et al.  Selection of viable cells with known DNA content. , 1980, Cytometry.

[20]  K. Bauer,et al.  Simultaneous measurement of cell cycle and apoptotic cell death. , 1998, Methods in cell biology.

[21]  P. Rabinovitch,et al.  Heterogeneity among T cells in intracellular free calcium responses after mitogen stimulation with PHA or anti-CD3. Simultaneous use of indo-1 and immunofluorescence with flow cytometry. , 1986, Journal of immunology.

[22]  S. C. Dixon,et al.  Bivariate flow cytometry of farm animal chromosomes: a potential tool for gene mapping. , 2009, Animal genetics.

[23]  M. H. Kaplan,et al.  LOCALIZATION OF A N T I G E N I N TISSUE CELLS I I . IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY*' , 2003 .

[24]  D J Arndt-Jovin,et al.  COMPUTER-CONTROLLED MULTIPARAMETER ANALYSIS AND SORTING OF CELLS AND PARTICLES , 1974, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[25]  A. Fauci,et al.  Detection and characterization of human high molecular weight B cell growth factor receptors on leukemic B cells in chronic lymphocytic leukemia. , 1989, The Journal of clinical investigation.

[26]  R. Feulgren,et al.  Mikroskopisch-chemischer Nachweis einer Nucleinsäure vom Typus der Thymonucleinsäure und die- darauf beruhende elektive Färbung von Zellkernen in mikroskopischen Präparaten. , 1924 .

[27]  T. T. Chen,et al.  Heterologous down-modulation of luteinizing hormone receptors by prolactin: a flow cytometry study. , 1991, Endocrinology.

[28]  L. Wheeless,et al.  Multidimensional slit-scan prescreening system: preliminary results of a single blind clinical study. , 1984, Cytometry.

[29]  B. Bohn.  Flow cytometry: A novel approach for the quantitative analysis of receptor-ligand interactions on surfaces of living cells , 1980, Molecular and Cellular Endocrinology.

[30]  C. Benz,et al.  Flow cytometric analysis of fluorescein-conjugated estradiol (E-BSA-FITC) binding in breast cancer suspensions. , 1985, Cytometry.

[31]  I. Blanca,et al.  Expression of different lipoprotein receptors in natural killer cells and their effect on natural killer proliferative and cytotoxic activity. , 1995, Immunology.

[32]  I J Christensen,et al.  Standardization of high-resolution flow cytometric DNA analysis by the simultaneous use of chicken and trout red blood cells as internal reference standards. , 1983, Cytometry.

[33]  E. Podniesinski,et al.  Amplification and detection of a Y-chromosome DNA sequence by fluorescence in situ polymerase chain reaction and flow cytometry using cells in suspension. , 1995, Cytometry.

[34]  B. Ponder,et al.  Complete characterization of a large marker chromosome by reverse and forward chromosome painting , 1992, Human Genetics.

[35]  Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry , 1997, Applied and environmental microbiology.

[36]  H. Gross,et al.  Model study detecting breast cancer cells in peripheral blood mononuclear cells at frequencies as low as 10(-7). , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[37]  M. Loken.  Separation of viable T and B lymphocytes using a cytochemical stain, Hoechst 33342. , 1980, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[38]  A. Órfão,et al.  Clinically useful information provided by the flow cytometric immunophenotyping of hematological malignancies: current status and future directions. , 1999, Clinical chemistry.

[39]  R. Duque.  Flow Cytometric Analysis of Lymphomas and Acute Leukemias , 1993, Annals of the New York Academy of Sciences.

[40]  L. Jenski,et al.  Anomalous changes in forward scatter of lymphocytes with loosely packed membranes. , 1999, Cytometry.

[41]  J. Gray,et al.  Is the central dogma of flow cytometry true: that fluorescence intensity is proportional to cellular dye content? , 1982, Cytometry.

[42]  J. Dyson,et al.  Characterization of six textile dyes as fluorescent stains for flow cytometry. , 1990, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[43]  L L Vindeløv,et al.  A review of techniques and results obtained in one laboratory by an integrated system of methods designed for routine clinical flow cytometric DNA analysis. , 1990, Cytometry.

[44]  L. Corash,et al.  Advances in leukocyte differential and peripheral blood stem cell enumeration , 1996, Current opinion in hematology.

[45]  I Nicoletti,et al.  A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. , 1991, Journal of immunological methods.

[46]  K. A. Kelley.  Very early detection of changes associated with cellular activation using a modified flow cytometer. , 1991, Cytometry.

[47]  S. Latt,et al.  Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. , 1976, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[48]  D. Pinkel,et al.  Bacterial characterization by flow cytometry. , 1983, Science.

[49]  L. Kamentsky,et al.  Spectrophotometer: New Instrument for Ultrarapid Cell Analysis , 1965, Science.

[50]  S. Demo,et al.  Quantitative measurement of mast cell degranulation using a novel flow cytometric annexin-V binding assay. , 1999, Cytometry.

[51]  Z. Darżynkiewicz,et al.  Expression of cyclins-B and cyclins-e in individual molt-4 cells and in stimulated human-lymphocytes during their progression through the cell-cycle. , 1993, International journal of oncology.

[52]  J. Elias,et al.  Cell proliferation indexes: a biomarker in solid tumors. , 1997, Biotechnic & histochemistry : official publication of the Biological Stain Commission.

[53]  H. Shapiro.  Cell membrane potential analysis. , 1994, Methods in cell biology.

[54]  P Valent,et al.  Up-regulation of LDL-receptor expression by LDL-immunoapheresis in patients with familial hypercholesterolemia. , 1999, Journal of investigative medicine : the official publication of the American Federation for Clinical Research.

[55]  W. Bushnell,et al.  Pontentiometric cyanine dyes are sensitive probes for mitochondria in intact plant cells : kinetin enhances mitochondrial fluorescence. , 1987, Plant physiology.

[56]  D. Hopwood,et al.  Cell and tissue fixation, 1972–1982 , 1985, The Histochemical Journal.

[57]  J. E. Celis,et al.  Cell Biology: A Laboratory Handbook , 1997 .

[58]  P. Lebaron,et al.  Comparison of Blue Nucleic Acid Dyes for Flow Cytometric Enumeration of Bacteria in Aquatic Systems , 1998, Applied and Environmental Microbiology.

[59]  M. Poot,et al.  Detection of apoptosis in live cells by MitoTracker red CMXRos and SYTO dye flow cytometry. , 1997, Cytometry.

[60]  B. Trask,et al.  High-speed chromosome sorting. , 1987, Science.

[61]  M. Mancini,et al.  Flow cytometric measurement of mitochondrial mass and function: A novel method for assessing chemoresistance , 1998, Annals of Surgical Oncology.

[62]  T Merrill,et al.  The LLNL high-speed sorter: design features, operational characteristics, and biological utility. , 1985, Cytometry.

[63]  S. Latt,et al.  New fluorochromes, compatible with high wavelength excitation, for flow cytometric analysis of cellular nucleic acids. , 1984, Cytometry.

[64]  H. Wijkström,et al.  Flow cytometric DNA and cytogenetic studies in human tumors: a comparison and discussion of the differences in modal values obtained by the two methods. , 1986, Cytometry.

[65]  Cram Ls.  Flow cytogenetics and chromosome sorting. , 1990 .

[66]  B. Drewinko,et al.  Comparison of in vitro methods to determine drug-induced cell lethality. , 1976, Cancer research.

[67]  T. Chused,et al.  Lymphocyte membrane potential and Ca2+‐sensitive potassium channels described by oxonol dye fluorescence measurements , 1985, Journal of cellular physiology.

[68]  M. Melamed,et al.  Analysis of apoptosis by laser scanning cytometry. , 1999, Cytometry.

[69]  E. Simons,et al.  Simultaneous measurement of stimulus-induced changes in cytoplasmic Ca2+ and in membrane potential of human neutrophils. , 1986, The Journal of biological chemistry.

[70]  J. Corsetti,et al.  The labeling of lipoproteins for studies of cellular binding with a fluorescent lipophilic dye. , 1991, Analytical biochemistry.

[71]  T. Welling,et al.  PKH26 and 125I-PKH95: characterization and efficacy as labels for in vitro and in vivo endothelial cell localization and tracking. , 1996, The Journal of surgical research.

[72]  H U Weier,et al.  Generation of five high-complexity painting probe libraries from flow-sorted mouse chromosomes. , 1994, Genomics.

[73]  H. P. Friedman.  The use of ultraviolet light and fluorescent dyes in the detection of uterine cancer by vaginal smear , 1950 .

[74]  R. Haugland,et al.  A novel fluorescent ceramide analogue for studying membrane traffic in animal cells: accumulation at the Golgi apparatus results in altered spectral properties of the sphingolipid precursor , 1991, The Journal of cell biology.

[75]  R. Ramponi,et al.  Fluorescence resonance energy transfer imaging as a tool for in situ evaluation of cell morphofunctional characteristics. , 1992, Journal of photochemistry and photobiology. B, Biology.

[76]  N J Dovichi,et al.  Light scatter detection within the sheath flow cuvette for size determination of multicomponent submicrometer particle suspensions. , 1987, Analytical chemistry.

[77]  J. Fried,et al.  Flow cytofluorometric analysis of cell cycle distributions using propidium iodide. Properties of the method and mathematical analysis of the data , 1976, The Journal of cell biology.

[78]  L. Mátyus,et al.  New trends in photobiology: Fluorescence resonance energy transfer measurements on cell surfaces. A spectroscopic tool for determining protein interactions , 1992 .

[79]  F. Bischoff,et al.  Rare event selection of fetal nucleated erythrocytes in maternal blood by flow cytometry. , 1996, Cytometry.

[80]  R. Murphy,et al.  Chapter 16 Flow-Cytometric Analysis of Endocytic Compartments , 1989 .

[81]  T. Lindmo,et al.  Flow cytometry and sorting , 1979 .

[82]  R. Murphy.  Analysis and isolation of endocytic vesicles by flow cytometry and sorting: demonstration of three kinetically distinct compartments involved in fluid-phase endocytosis. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[83]  D. Shouval,et al.  Fluidity of membrane lipids and lateral mobility of concanavalin A receptors in the cell surface of normal lymphocytes and lymphocytes from patients with malignant lymphomas and leukemias. , 1977, Cancer research.

[84]  R. Leif,et al.  The use of antibody specific for bromodeoxyuridine for the immunofluorescent determination of DNA replication in single cells and chromosomes. , 1975, Experimental cell research.

[85]  A. Landay,et al.  Procedural guidelines for performing immunophenotyping by flow cytometry. , 1989, Clinical immunology and immunopathology.

[86]  L. Mcgann,et al.  Light scattering and cell volumes in osmotically stressed and frozen-thawed cells. , 1988, Cytometry.

[87]  P. Steinlein,et al.  Cell biologists sort things out: Analysis and purification of intracellular organelles by flow cytometry. , 1997, Trends in cell biology.

[88]  A. Harel-Bellan,et al.  Flow cytometry resonance energy transfer suggests an association between low-affinity interleukin 2 binding sites and HLA class I molecules. , 1990, The Biochemical journal.

[89]  H. Shapiro.  Flow cytometric estimation of DNA and RNA content in intact cells stained with Hoechst 33342 and pyronin Y. , 1981, Cytometry.

[90]  T. Shenk,et al.  Use of a membrane-localized green fluorescent protein allows simultaneous identification of transfected cells and cell cycle analysis by flow cytometry. , 1997, Cytometry.

[91]  G. Finkel,et al.  Cytologic screening automated by Cytoscreener. , 1970, JAAMI : journal of the Association for the Advancement of Medical Instrumentation.

[92]  H. Rinneberg,et al.  Flow cytometric differentiation of erythrocytes and leukocytes in dilute whole blood by light scattering. , 1998, Cytometry.

[93]  S. Abdullah,et al.  Comparison of different staining procedures for the flow cytometric analysis of U-937 cells infected with different Leishmania-species. , 1999, Journal of microbiological methods.

[94]  M. Hilchenbach,et al.  Simple method for comparing large numbers of flow cytometry histograms exemplified by analysis of the CD4 (T4) antigen and LDL receptor on human peripheral blood lymphocytes. , 1986, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[95]  W. Russell,et al.  A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses , 1975, Nature.

[96]  J. Steinkamp,et al.  A new multiparameter separator for microscopic particles and biological cells. , 1973, The Review of scientific instruments.

[97]  Michael G. Ormerod,et al.  Flow Cytometry: A Practical Approach , 1994 .

[98]  H. Crissman,et al.  Specific staining of DNA with the fluorescent antibiotics, mithramycin, chromomycin, and olivomycin. , 1990, Methods in cell biology.

[99]  W. Göhde,et al.  Separation of Pancreatic Islets by Fluorescence-Activated Sorting , 1989, Diabetes.

[100]  Z. Darżynkiewicz.  Simultaneous analysis of cellular RNA and DNA content. , 1994, Methods in cell biology.

[101]  D. Galbraith,et al.  Reference standards for determination of DNA content of plant nuclei. , 1999, American journal of botany.

[102]  R. Beelen,et al.  Separation of alveolar macrophages and dendritic cells via autofluorescence: phenotypical and functional characterizatior , 1993, Journal of leukocyte biology.

[103]  A. Verkman,et al.  Membrane chloride transport measured using a chloride-sensitive fluorescent probe. , 1987, Biochemistry.

[104]  E. Peterman,et al.  Optical methods for exploring dynamics of single copies of green fluorescent protein. , 1999, Cytometry.

[105]  H. Tanke,et al.  Intracellular pH-determination by fluorescence measurements. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[106]  B H Mayall,et al.  Chromosome measurement and sorting by flow systems. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[107]  D. Lester,et al.  Social modification of alcohol consumption in inbred mice. , 1975, Science.

[108]  M. Melamed,et al.  New cell cycle compartments identified by multiparameter flow cytometry. , 1980, Cytometry.

[109]  G. Brecher,et al.  Evaluation of electronic red blood cell counter. , 1956, American journal of clinical pathology.

[110]  P. Rabinovitch,et al.  Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-amino-actinomycin D and single laser excitation: applications to study of cell activation and the cell cycle of murine Ly-1 B cells. , 1986, Journal of immunology.

[111]  M. Shinitzky,et al.  Microviscosity parameters and protein mobility in biological membranes. , 1976, Biochimica et biophysica acta.

[112]  Roger Y. Tsien,et al.  Improved green fluorescence , 1995, Nature.

[113]  F. Lacombe,et al.  Flow cytometry study of cell cycle, apoptosis and drug resistance in acute leukemia. , 1996, Hematology and cell therapy.

[114]  I. Bertoncello,et al.  Fluoro-Gold: An alternative viability stain for multicolor flow cytometric analysis. , 1999, Cytometry.

[115]  G. Valet,et al.  Flow-cytometric determination of glutathione alterations in vital cells by o-phthaldialdehyde (OPT) staining. , 1986, Experimental cell research.

[116]  M. Melamed,et al.  Size and refractive index dependence of simple forward angle scattering measurements in a flow system using sharply-focused illumination. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[117]  J. Padmanabhan,et al.  Flow cytometric analysis of nitric oxide production in human neutrophils using dichiorofluorescein diacetate in the presence of a calmodulin inhibitor , 2005 .

[118]  G. Nolan,et al.  Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[119]  S. Heimfeld,et al.  Use of fluorescence threshold triggering and high-speed flow cytometry for rare event detection. , 1995, Cytometry.

[120]  J. Lakowicz,et al.  Phase-resolved fluorescence lifetime measurements for flow cytometry. , 1993, Cytometry.

[121]  M. Melamed,et al.  Accessibility of DNA in situ to various fluorochromes: relationship to chromatin changes during erythroid differentiation of Friend leukemia cells. , 1984, Cytometry.

[122]  J. Hoffman,et al.  Determination of membrane potentials in human and Amphiuma red blood cells by means of a fluorescent probe , 1974, The Journal of physiology.

[123]  A. Monaco,et al.  Construction, arraying, and high-density screening of large insert libraries of human chromosomes X and 21: their potential use as reference libraries. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[124]  T. Caspersson.  Cell growth and cell function , 1950 .

[125]  J. O'connor,et al.  Study of surface carbohydrates on isolated Golgi subfractions by fluorescent-lectin binding and flow cytometry. , 1995, Cytometry.

[126]  R. C. Benson,et al.  Cellular autofluorescence--is it due to flavins? , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[127]  L O Henderson,et al.  Terminology and nomenclature for standardization in quantitative fluorescence cytometry. , 1998, Cytometry.

[128]  K. Anderson,et al.  Reversal of multidrug resistance phenotype by surfactants: relationship to membrane lipid fluidity. , 1995, Archives of biochemistry and biophysics.

[129]  K. Sigler,et al.  Slow fluorescent indicators of membrane potential: a survey of different approaches to probe response analysis. , 1996, Journal of photochemistry and photobiology. B, Biology.

[130]  C B Bagwell,et al.  DNA histogram debris theory and compensation. , 1991, Cytometry.

[131]  J. Visser,et al.  Purification of murine hemopoietic stem cells and committed progenitors by fluorescence activated cell sorting using wheat germ agglutinin and monoclonal antibodies. , 1988, Acta histochemica. Supplementband.

[132]  D. Hassall.  Three probe flow cytometry of a human foam-cell forming macrophage. , 1992, Cytometry.

[133]  J. Gray,et al.  Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle. , 1986, Experimental cell research.

[134]  A Masini,et al.  Functional heterogeneity of an isolated mitochondrial population revealed by cytofluorometric analysis at the single organelle level. , 1996, Experimental cell research.

[135]  G. Jürgens,et al.  High density lipoprotein uptake by freshly isolated human peripheral blood T lymphocytes. , 1987, Immunobiology.

[136]  M. Ormerod.  The study of apoptotic cells by flow cytometry , 1998, Leukemia.

[137]  S. Azen,et al.  Leukocyte immunophenotyping by flow cytometry in a multisite study: standardization, quality control, and normal values in the Transfusion Safety Study. The Transfusion Safety Study Group. , 1990, Clinical immunology and immunopathology.

[138]  M. Melamed,et al.  Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[139]  K. Sakamoto,et al.  Analysis with flow cytometry of green fluorescent protein expression in leukemic cells. , 1999, Cytometry.

[140]  R. Tsien,et al.  green fluorescent protein , 2020, Catalysis from A to Z.

[141]  M. Andreeff,et al.  Consensus review of the clinical utility of DNA flow cytometry in neoplastic hematopathology. , 1993, Cytometry.

[142]  T. Hammond,et al.  Permeability properties of the mammalian bladder apical membrane. , 1994, The American journal of physiology.

[143]  D. Hedley,et al.  DNA Cytometry Consensus Conference. Consensus review of the clinical utility of DNA cytometry in carcinoma of the breast. , 1993, Breast cancer research and treatment.

[144]  B. D. de Grooth,et al.  Photon-counting device compatible with conventional flow cytometric data acquisition electronics. , 1998, Cytometry.

[145]  Z. Darżynkiewicz,et al.  Critical aspects in the analysis of apoptosis and necrosis. , 1998, Human cell.

[146]  M. Edidin,et al.  Energy transfer methods for detecting molecular clusters on cell surfaces. , 1997, Methods in enzymology.

[147]  L. Wheeless,et al.  DNA cytometry consensus conference. , 1993, Cytometry.

[148]  S. Damjanovich,et al.  Proximity measurements of cell surface proteins by fluorescence energy transfer. , 1987, Immunology letters.

[149]  I. Taylor.  A rapid single step staining technique for DNA analysis by flow microfluorimetry. , 1980, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[150]  T. Brüning,et al.  Flow-cytometric determination of high-density-lipoprotein binding sites on human leukocytes. , 1987, Clinical chemistry.

[151]  H. Zola,et al.  Detection of cytokine receptors by high-sensitivity immunofluorescence/flow cytometry. , 1992, Immunobiology.

[152]  P. Horan,et al.  Fluorescent cell labeling for in vivo and in vitro cell tracking. , 1990, Methods in cell biology.

[153]  J. Y. Chin,et al.  Aequorea green fluorescent protein analysis by flow cytometry. , 1995, Cytometry.

[154]  David F. Keren,et al.  Flow Cytometry in Clinical Diagnosis , 1994 .

[155]  W. Kosenow.  Die Fluorochromierung mit Acridinorange, eine Methode zur Lebend-beobachtung gefärbter Blutzellen , 1952 .

[156]  J. McCoy,et al.  Current practices in clinical flow cytometry. A practice survey by the American Society of Clinical Pathologists. , 1999, American journal of clinical pathology.

[157]  B. Thorell.  Intracellular red-ox steady states as basis for cell characterization by flow cytofluorometry. , 1980, Blood cells.

[158]  A Krishan,et al.  Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining , 1975, The Journal of cell biology.

[159]  R C MELLORS,et al.  A micro-fluorometric scanner for the differential detection of cells; application of exfoliative cytology. , 1951, Science.

[160]  V Kachel,et al.  A new flow cytometric transducer for fast sample throughput and time resolved kinetic studies of biological cells and other particles. , 1982, Cytometry.

[161]  J. Yewdell,et al.  Fluorescent conjugates of brefeldin A selectively stain the endoplasmic reticulum and Golgi complex of living cells. , 1995, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[162]  J. Uličný.  Lorenz-Mie light scattering in cellular biology. , 1992, General physiology and biophysics.

[163]  T. Hirschfeld.  Optical microscopic observation of single small molecules. , 1976, Applied optics.

[164]  B. J. Price,et al.  Multiangle light scattering flow photometry of cultured human fibroblasts: comparison of normal cells with a mutant line containing cytoplasmic inclusions. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[165]  D. J. Mason,et al.  A Fluorescent Gram Stain for Flow Cytometry and Epifluorescence Microscopy , 1998, Applied and Environmental Microbiology.

[166]  C. Parish,et al.  Fluorescent dyes for lymphocyte migration and proliferation studies , 1999, Immunology and cell biology.

[167]  L. Wheeless,et al.  Consensus review of the clinical utility of DNA cytometry in bladder cancer. Report of the DNA Cytometry Consensus Conference. , 1993, Cytometry.

[168]  J F Leary,et al.  Laser flow cytometric light scatter and fluorescence pulse width and pulse rise-time sizing of mammalian cells. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[169]  D. Pipeleers,et al.  Islet cell analysis and purification by light scatter and autofluorescence. , 1982, Biochemical and biophysical research communications.

[170]  H. Tanke,et al.  Flow cytometry of human reticulocytes based on RNA fluorescence. , 1981, Cytometry.

[171]  W. J. Visser,et al.  Isolation of autofluorescent "aged" human fibroblasts by flow sorting. Morphology, enzyme activity and proliferative capacity. , 1982, Experimental cell research.

[172]  T. Kuroiwa,et al.  Behavior of mitochondria, chloroplasts and their nuclei during the mitotic cycle in the ultramicroalga Cyanidioschyzon merolae. , 1994, European journal of cell biology.

[173]  Z. Darżynkiewicz,et al.  Assays of cell viability: discrimination of cells dying by apoptosis. , 1994, Methods in cell biology.

[174]  J. O'connor,et al.  A flow cytometric assay for intracellular nonprotein thiols using mercury orange. , 1988, Cytometry.

[175]  E. Banfi,et al.  Simultaneous flow cytometric method to measure phagocytosis and oxidative products by neutrophils. , 1991, Cytometry.

[176]  W Stokdijk,et al.  Optical plankton analyser: a flow cytometer for plankton analysis, II: Specifications. , 1989, Cytometry.

[177]  Brian H. Mayall,et al.  Guidelines for implementation of clinical DNA cytometry , 1993 .

[178]  A. Sartorelli,et al.  Differentiation of HL-60 promyelocytic leukemia cells monitored by flow cytometric measurement of nitro blue tetrazolium (NBT) reduction. , 1985, Cytometry.

[179]  J. Steinkamp,et al.  Flow microfluorometric studies of lectin binding to mammalian cells II. Estimation of the surface density of receptor sites by multiparameter analysis , 1974, Journal of cellular physiology.

[180]  C. M. Yentsch.  Environmental health: flow cytometric methods to assess our water world. , 1990, Methods in cell biology.

[181]  S. Je.  Lies, damned lies--and biological stains. , 1972 .

[182]  R. Mathies,et al.  Heterodimeric DNA-binding dyes designed for energy transfer: stability and applications of the DNA complexes. , 1993, Nucleic acids research.

[183]  P. Petit,et al.  Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis , 1995, The Journal of cell biology.

[184]  L. Wheeless,et al.  Quantitative single cell analysis and sorting. , 1977, Science.

[185]  L. Stryer,et al.  Energy transfer: a spectroscopic ruler. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[186]  J. Paul Robinson,et al.  Handbook of Flow Cytometry Methods , 1993 .

[187]  I. Thorneycroft,et al.  Preconceptional sex selection: past, present, and future. , 1998, Archives of andrology.

[188]  R. W. Horobin,et al.  The impurities of biological dyes: their detection, removal, occurrence and histological significance—a review , 1969, The Histochemical Journal.

[189]  H. Crissman,et al.  Detailed studies on the application of three fluorescent antibiotics for DNA staining in flow cytometry. , 1978, Stain technology.

[190]  M. Fulwyler,et al.  Electronic Separation of Biological Cells by Volume , 1965, Science.

[191]  R. Hoffman,et al.  Resolution of dimly fluorescent particles: a practical measure of fluorescence sensitivity. , 1998, Cytometry.

[192]  R. Chatelier,et al.  Calibration of flow cytometric fluorescence standards using the isoparametric analysis of ligand binding. , 1987, Cytometry.

[193]  M. Roederer,et al.  CD4 and CD8 T cells with high intracellular glutathione levels are selectively lost as the HIV infection progresses , 1991 .

[194]  R Y Tsien,et al.  Fluorescent indicators for cytosolic sodium. , 1989, The Journal of biological chemistry.

[195]  A. L. Koch,et al.  Determination of the Biomasses of Small Bacteria at Low Concentrations in a Mixture of Species with Forward Light Scatter Measurements by Flow Cytometry , 1998, Applied and Environmental Microbiology.

[196]  M. Ferguson-Smith.  Genetic Analysis by Chromosome Sorting and Painting: Phylogenetic and Diagnostic Applications , 1997, European journal of human genetics : EJHG.

[197]  R. G. Sweet.  High Frequency Recording with Electrostatically Deflected Ink Jets , 1965 .

[198]  Joseph S. Fruton,et al.  The Collected Papers of Paul Ehrlich , 1957, The Yale Journal of Biology and Medicine.

[199]  J. Sparks,et al.  Cellular heterogeneity in binding and uptake of low‐density lipoprotein in primary rat hepatocytes , 1993, Hepatology.

[200]  G C Salzman,et al.  A flow-system multiangle light-scattering instrument for cell characterization. , 1975, Clinical chemistry.

[201]  J. Aubin.  Autofluorescence of viable cultured mammalian cells. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[202]  B. Trask,et al.  Quantification by flow cytometry of chromosome-17 deletions in Smith-Magenis syndrome patients , 1996, Human Genetics.

[203]  A. Landay.  Clinical flow cytometry , 1993 .

[204]  L. A. Kamentsky,et al.  Instrumentation for automated examinations of cellular specimens , 1969 .

[205]  S. Sahlin,et al.  Differentiation between attached and ingested immune complexes by a fluorescence quenching cytofluorometric assay. , 1983, Journal of immunological methods.

[206]  K. Mcleish,et al.  A simultaneous flow cytometric measurement of neutrophil phagocytosis and oxidative burst in whole blood. , 1987, Diagnostic and clinical immunology.

[207]  M P Lee,et al.  Light scattering and fluorescence by small particles having internal structure. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[208]  L. Herzenberg,et al.  ANALYSIS OF CELL POPULATIONS WITH A FLUORESCENCE‐ACTIVATED CELL SORTER * , 1975, Annals of the New York Academy of Sciences.

[209]  C Nicolini,et al.  A comparison of mathematical methods for the analysis of DNA histograms obtained by flow cytometry , 1982, Cell and tissue kinetics.

[210]  R. Higashikubo,et al.  Cell-cycle position and nuclear protein content. , 1982, Cytometry.

[211]  J H Jett,et al.  Detection of single molecules of phycoerythrin in hydrodynamically focused flows by laser-induced fluorescence. , 1987, Analytical chemistry.

[212]  M. Roth,et al.  A fluorescent lipid analogue can be used to monitor secretory activity and for isolation of mammalian secretion mutants. , 1995, Molecular biology of the cell.

[213]  G. Valet,et al.  Flow-cytometric characterization of stimulation, free radical formation, peroxidase activity and phagocytosis of human granulocytes with 2,7-dichlorofluorescein (DCF). , 1987, European journal of cell biology.

[214]  K. Zatloukal,et al.  High molecular weight components are main constituents of Mallory bodies isolated with a fluorescence activated cell sorter. , 1991, Laboratory investigation; a journal of technical methods and pathology.

[215]  C. Stewart,et al.  Use of a photolabeling technique to identify nonviable cells in fixed homologous or heterologous cell populations. , 1991, Cytometry.

[216]  H. Crissman,et al.  TOTO and YOYO: new very bright fluorochromes for DNA content analyses by flow cytometry. , 1994, Cytometry.

[217]  R. Haugland,et al.  Green- and red-fluorescent nanospheres for the detection of cell surface receptors by flow cytometry. , 1998, Journal of immunological methods.

[218]  K. Teague,et al.  Comparative flow cytometric analysis of proliferating cell nuclear antigen (PCNA) antibodies in human solid neoplasms. , 1994, Cytometry.

[219]  M. Melamed,et al.  Consensus review of the clinical utility of DNA flow cytometry in colorectal cancer. , 1993, Cytometry.

[220]  M. Seeds,et al.  Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. , 1983, Journal of immunology.

[221]  J. Jacobberger,et al.  Analysis of intracellular antigens by flow cytometry. , 1986, Cytometry.

[222]  M. Melamed,et al.  Flow cytofluorimetry: discrimination between single cells and cell aggregates by direct size measurements. , 1975, Acta cytologica.

[223]  M H Muser,et al.  Time-resolved flow cytometry for the measurement of lanthanide chelate fluorescence: II. Instrument design and experimental results. , 1994, Cytometry.

[224]  C. Cox,et al.  Correction of cellular autofluorescence in flow cytometry by mathematical modeling of cellular fluorescence. , 1988, Cytometry.

[225]  C F Sams,et al.  Development of a whole blood staining device for use during space shuttle flights. , 1999, Cytometry.

[226]  M. Donze,et al.  Anomalous behaviour of forward and perpendicular light scattering of a cyanobacterium owing to intracellular gas vacuoles. , 1987, Cytometry.

[227]  J V Watson,et al.  Improved methodology for intracellular enzyme reaction and inhibition kinetics by flow cytometry. , 1987, Cytometry.

[228]  H. Petty,et al.  Imaging neutrophil activation: Analysis of the translocation and utilization of NAD(P)H‐associated autofluorescence during antibody‐dependent target oxidation , 1992, Journal of cellular physiology.

[229]  M. Chalfie,et al.  Green fluorescent protein as a marker for gene expression. , 1994, Science.

[230]  L A Sklar,et al.  A convenient on-line device for reagent addition, sample mixing, and temperature control of cell suspensions in flow cytometry. , 1985, Cytometry.

[231]  Robert C. Leif,et al.  Convention on nomenclature for DNA cytometry , 1984 .

[232]  J. Gray,et al.  Techniques in Cell Cycle Analysis , 1987, Humana Press.

[233]  A. Graham,et al.  Changes in free cholesterol content, measured by filipin fluorescence and flow cytometry, correlate with changes in cholesterol biosynthesis in THP-1 macrophages. , 1995, Cytometry.

[234]  B. J. Price,et al.  Light-scatter analysis of microalgae. Correlation of scatter patterns from pure and mixed asynchronous cultures. , 1978, Biophysical journal.

[235]  T M Jovin,et al.  Fluorescence labeling and microscopy of DNA. , 1989, Methods in cell biology.

[236]  P. Henkart,et al.  Fluidity of cell membranes--current concepts and trends. , 1979, International review of cytology.

[237]  G Böck,et al.  Comparison of low density lipoprotein uptake by different human lymphocyte subsets: a new method using double-fluorescence staining. , 1996, Journal of lipid research.

[238]  D. Hedley,et al.  Flow cytometric measurement of cytoplasmic pH: a critical evaluation of available fluorochromes. , 1986, Cytometry.

[239]  B. Trask,et al.  Characterization of somatic cell hybrids by bivariate flow karyotyping and fluorescencein situ hybridization , 1991, Somatic Cell Genetics.

[240]  L. Mátyus,et al.  Flow cytometric measurements of fluorescence energy transfer using single laser excitation. , 1987, Cytometry.

[241]  J. Stellmach,et al.  Impulscytofluorometrie der Redox-Aktivität von Einzelzellen mit einem neuen fluorescierenden Formazan , 1984 .

[242]  W. Bonner,et al.  Cell Sorting: Automated Separation of Mammalian Cells as a Function of Intracellular Fluorescence , 1969, Science.

[243]  H. Maeda,et al.  A new simple fluorometric assay for phagocytosis. , 1986, Journal of immunological methods.

[244]  H M Shapiro,et al.  Detection and discrimination of individual viruses by flow cytometry. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[245]  P. Niederer,et al.  Time-resolved flow cytometry for the measurement of lanthanide chelate fluorescence: I. Concept and theoretical evaluation. , 1994, Cytometry.

[246]  A. Look,et al.  The cytogenetics of childhood leukemia: clinical and biologic implications. , 1988, Pediatric clinics of North America.

[247]  T. J. Fraser,et al.  Comparison of different methods of determining cell viability after exposure to cytotoxic compounds. , 1976, Experimental cell research.

[248]  Frank Dolbeare,et al.  Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics , 2004, The Histochemical Journal.

[249]  L. Mátyus,et al.  Application of fluorescence resonance energy transfer in the clinical laboratory: routine and research. , 1998, Cytometry.

[250]  G van den Engh,et al.  Fluorescence spectra of DNA dyes measured in a flow cytometer. , 1996, Cytometry.

[251]  A. Helmberg,et al.  An orally administered bacterial immunomodulator primes rabbit neutrophils for increased oxidative burst in response to opsonized zymosan , 1989, Infection and immunity.

[252]  M. Ormerod.  Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-ethidium bromide method. , 1997, Methods in molecular biology.

[253]  P. F. Mullaney,et al.  Cell Microfluorometry: A Method for Rapid Fluorescence Measurement , 1969, Science.

[254]  T. Caspersson,et al.  Nucleic Acid Metabolism of the Chromosomes in Relation to Gene Reproduction , 1938, Nature.

[255]  J. O'connor,et al.  Flow cytometric analysis of concanavalin A binding to isolated Golgi fractions from rat liver. , 1993, Experimental cell research.

[256]  D. Loo,et al.  Measurement of cell death. , 1998, Methods in cell biology.

[257]  F. D. De Schryver,et al.  Photophysics of the fluorescent K+ indicator PBFI. , 1995, Biophysical journal.

[258]  S. Demers,et al.  Particle analysis in oceanography , 1991 .

[259]  L L Wheeless,et al.  A multidimensional slit-scan flow system. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[260]  V. Barsky,et al.  7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry. , 1984, Cytometry.

[261]  M. Melamed,et al.  Effect of section thickness on quality of flow cytometric DNA content determinations in paraffin-embedded tissues. , 1986, Cytometry.

[262]  M. Melamed,et al.  Laser-scanning cytometry: A new instrumentation with many applications. , 1999, Experimental cell research.

[263]  H. Crissman,et al.  Application of pyronin Y(G) in cytochemistry of nucleic acids. , 1987, Cytometry.

[264]  M. Stetler-Stevenson,et al.  Quantitative fluorescence: to count or not to count. Is that the question? , 1998, Cytometry.

[265]  L. Kamentsky,et al.  Efficacy of a rapid cell spectrophotometer in screening for cervical cancer , 1968, Cancer.

[266]  R. Veselý,et al.  Initial results of Central European Immunophenotyping Quality Control Program (CEQUAL). , 1996, Cytometry.

[267]  T. Smith,et al.  J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. , 1991, Biochemistry.

[268]  I. Taylor,et al.  Application of DNA flow cytometry to paraffin-embedded archival material for the study of aneuploidy and its clinical significance. , 1985, Cytometry.

[269]  R. Jones,et al.  Immunological Properties of an Antibody Containing a Fluorescent Group.∗ , 1941 .

[270]  S. Fey,et al.  Cyclin: a nuclear protein whose level correlates directly with the proliferative state of normal as well as transformed cells. , 1984, Leukemia research.

[271]  M. Roederer,et al.  8 color, 10-parameter flow cytometry to elucidate complex leukocyte heterogeneity. , 1997, Cytometry.

[272]  D Barnett,et al.  Flow cytometric quantitation of immunofluorescence intensity: problems and perspectives. European Working Group on Clinical Cell Analysis. , 1998, Cytometry.

[273]  G. Pratt,et al.  Modern molecular diagnostics and the management of haematological malignancies. , 1998, Clinical and laboratory haematology.

[274]  R. Sgonc,et al.  Apoptosis detection: an overview , 1998, Experimental Gerontology.

[275]  M R Loken,et al.  Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. , 1990, Cytometry.

[276]  I. Christensen,et al.  A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. , 1983, Cytometry.

[277]  Z. Darżynkiewicz,et al.  Features of apoptotic cells measured by flow cytometry. , 1992, Cytometry.

[278]  J. F. Keane,et al.  Nucleic acid content of the squamous cancer cell. , 1952, Science.

[279]  T. Tsuruo,et al.  Improved flow-cytometric detection of low P-glycoprotein expression in leukaemic blasts by histogram subtraction analysis. , 1994, Cytometry.

[280]  M. Melamed,et al.  Estimation of cell size from pulse shape in flow cytofluorometry. , 1976, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[281]  D. McClish,et al.  Altered membrane anisotropy gradients of plasma membranes of living peripheral blood leukocytes in aging and Alzheimer's Disease , 1991, Mechanisms of Ageing and Development.

[282]  A Schwartz,et al.  Development of Clinical Standards for Flow Cytometry , 1993, Annals of the New York Academy of Sciences.

[283]  L. Chen.  Chapter 7 Fluorescent Labeling of Mitochondria , 1988 .

[284]  V. Dill,et al.  Flow cytometry : instrumentation and data analysis , 1987 .

[285]  Howard M. Shapiro,et al.  Practical Flow Cytometry , 1985 .

[286]  G C Salzman,et al.  Cell classification by laser light scattering: identification and separation of unstained leukocytes. , 1975, Acta cytologica.

[287]  I J Christensen,et al.  Limits of detection of nuclear DNA abnormalities by flow cytometric DNA analysis. Results obtained by a set of methods for sample-storage, staining and internal standardization. , 1983, Cytometry.

[288]  B. Bohn.  High-sensitivity cytofluorometric quantitation of lectin and hormone binding to surfaces of living cells. , 1976, Experimental cell research.

[289]  C. Milstein,et al.  Continuous cultures of fused cells secreting antibody of predefined specificity , 1975, Nature.

[290]  M. Lalande,et al.  Fluorescence flow analysis of lymphocyte activation using Hoechst 33342 dye. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[291]  T M Chused,et al.  Voltage‐sensitive cyanine dye fluorescence signals in lymphocytes: Plasma membrane and mitochondrial components , 1985, Journal of cellular physiology.

[292]  K A Kelley,et al.  Sample station modification providing on-line reagent addition and reduced sample transit time for flow cytometers. , 1989, Cytometry.

[293]  J. Stegemann,et al.  A new fluid switching flow sorter , 2004, Histochemistry.

[294]  G. Valet,et al.  Determination of intracellular calcium in vital cells by flow-cytometry , 1985, Naturwissenschaften.

[295]  T. Stokke,et al.  Distinction of leucocyte classes based on chromatin-structure-dependent DNA-binding of 7-aminoactinomycin D. , 1987, Cytometry.

[296]  Gregor Rothe,et al.  Flow Cytometric Analysis of Respiratory Burst Activity in Phagocytes With Hydroethidine and 2′,7′‐Dichlorofluorescin , 1990, Journal of leukocyte biology.

[297]  H. Pralle,et al.  Relative and Absolute Changes of Activated Platelets, Microparticles and Platelet Aggregates after Activation in vitro , 1998, Pathophysiology of Haemostasis and Thrombosis.

[298]  D. Balding,et al.  HLA Sequence Polymorphism and the Origin of Humans , 2006 .

[299]  B. Trask,et al.  The binding kinetics and interaction of DNA fluorochromes used in the analysis of nuclei and chromosomes by flow cytometry , 1986, Histochemistry.

[300]  J. Gray,et al.  Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[301]  K. Widhalm,et al.  Analysis of fluorescent low density lipoprotein uptake by lymphocytes, paradoxical increase in the elderly , 1987, Mechanisms of Ageing and Development.

[302]  M. Van Dilla,et al.  Volume Distribution and Separation of Normal Human Leucocytes.∗ , 1967, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[303]  T. Jovin,et al.  Analysis and sorting of living cells according to deoxyribonucleic acid content. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[304]  G. Wick,et al.  Simultaneous determination of cell surface antigens and apoptosis. , 1994, Trends in genetics : TIG.

[305]  J. Stellmach,et al.  [Impulse cytofluorometry of the redox activity of single cells using fluorescent formazan]. , 1984, Acta histochemica.

[306]  C. Reutelingsperger,et al.  Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. , 1998, Cytometry.

[307]  P. Medawar,et al.  ‘Actively Acquired Tolerance’ of Foreign Cells , 1953, Nature.

[308]  D. Peters,et al.  A comparison of mercury arc lamp and laser illumination for flow cytometers. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[309]  G. Papanicolaou,et al.  The diagnostic value of vaginal smears in carcinoma of the uterus. 1941. , 1941, Archives of pathology & laboratory medicine.

[310]  I W Taylor,et al.  Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[311]  J. Watson.  Enzyme kinetic studies in cell populations using fluorogenic substrates and flow cytometric techniques. , 1980, Cytometry.

[312]  F J Otto,et al.  High-resolution analysis of nuclear DNA employing the fluorochrome DAPI. , 1994, Methods in cell biology.

[313]  Z. Darżynkiewicz,et al.  Changes in accessibility of DNA to various fluorochromes during spermatogenesis. , 1986, Cytometry.

[314]  F. Lauer,et al.  Uses and future applications of flow cytometry in immunotoxicity testing. , 1999, Methods.

[315]  L D Bogh,et al.  Flow cytometry instrumentation in research and clinical laboratories. , 1993, Clinical laboratory science : journal of the American Society for Medical Technology.

[316]  J. Watson,et al.  Flow Cytometry Data Analysis: Basic Concepts and Statistics , 1992 .

[317]  M. Grace,et al.  Three-color flow cytometry analysis of tricistronic expression of eBFP, eGFP, and eYFP using EMCV-IRES linkages. , 1999, Cytometry.

[318]  M. Poot,et al.  Cell cycle analysis using continuous bromodeoxyuridine labeling and Hoechst 33258-ethidium bromide bivariate flow cytometry. , 1990, Methods in cell biology.

[319]  C. Franceschi,et al.  A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1). , 1993, Biochemical and biophysical research communications.

[320]  K. Tsou,et al.  A comparative study of DAPI, DIPI, and Hoechst 33258 and 33342 as chromosomal DNA stains. , 1985, Stain technology.

[321]  W. Bolton,et al.  Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry. , 1995, Cytometry.

[322]  G. Steele,et al.  Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[323]  P. Rabinovitch,et al.  Chapter 10 Intracellular Ionized Calcium , 1994 .

[324]  H. Crissman,et al.  Cell-Cycle Analysis in 20 Minutes , 1974, Science.

[325]  J V Giorgi,et al.  Quantitation of CD38 activation antigen expression on CD8+ T cells in HIV-1 infection using CD4 expression on CD4+ T lymphocytes as a biological calibrator. , 1998, Cytometry.

[326]  C. chou,et al.  Is green fluorescent protein toxic to the living cells? , 1999, Biochemical and biophysical research communications.

[327]  A. Partin,et al.  Correlation of prognosis to nuclear roundness and to flow cytometric light scatter. , 1987, Analytical and quantitative cytology and histology.

[328]  E. Sugarbaker,et al.  Preparation of tissues for DNA flow cytometric analysis. , 1980, Cytometry.

[329]  J A Hokanson,et al.  Theoretical basis for sampling statistics useful for detecting and isolating rare cells using flow cytometry and cell sorting. , 1997, Cytometry.

[330]  A. Verkerk,et al.  Cell sorting and microchemistry of cultured human fibroblasts: applications in genetics and aging research. , 1984, Cytometry.

[331]  M. Macey.  Flow cytometry : clinical applications , 1993 .

[332]  A. Tsukamoto,et al.  Development of a clinically applicable high-speed flow cytometer for the isolation of transplantable human hematopoietic stem cells. , 1995, Journal of hematotherapy.

[333]  I. Taylor,et al.  DNA flow cytometry of paraffin-embedded tissue. , 1984, Cytometry.

[334]  W. Hiddemann,et al.  Convention on nomenclature for DNA cytometry , 1984 .

[335]  O. Laerum,et al.  Flow cytometry in hematology , 1992 .

[336]  L. Johnson.  Sex preselection by flow cytometric separation of X and Y chromosome-bearing sperm based on DNA difference: a review. , 1995, Reproduction, fertility, and development.

[337]  O. Kallioniemi,et al.  Consensus review of the clinical utility of DNA content cytometry in prostate cancer. , 1993, Cytometry.

[338]  M. Tiirikainen.  Evaluation of red blood cell lysing solutions for the detection of intracellular antigens by flow cytometry. , 1995, Cytometry.

[339]  D. Bostwick,et al.  Potential markers of aggressiveness in prostatic intraepithelial neoplasia detected by fluorescence in situ hybridization. , 1996, European urology.

[340]  I Schmid,et al.  Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. , 1992, Cytometry.

[341]  G. Frelat,et al.  Analysis and sorting of chromosomes by flow cytometry: New trends , 1993, Biology of the cell.

[342]  F. Goot,et al.  Flow cytometry and sorting of amphibian bladder endocytic vesicles containing ADH-sensitive water channels , 1992, The Journal of Membrane Biology.

[343]  M. Loken.  Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. , 1980, Cytometry.

[344]  N. King,et al.  Flow cytometric analysis of immunogold cell surface label. , 1984, Cytometry.

[345]  J. Kao,et al.  Practical aspects of measuring [Ca2+] with fluorescent indicators. , 1994, Methods in cell biology.

[346]  L. Huber,et al.  Use of a FACS III for fluorescence depolarization with DPH. , 1989, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[347]  H. Shapiro.  Chapter 8 Cell Membrane Potential Analysis , 1994 .

[348]  D. Huhn,et al.  Quantitative fluorescence flow cytometry: a comparison of the three techniques for direct and indirect immunofluorescence. , 1998, Cytometry.

[349]  E. Modest,et al.  7-Amino-actinomycin D as a cytochemical probe. I. Spectral properties. , 1975, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[350]  J. van Egmond,et al.  Simplified method for DNA and protein staining of human hematopoietic cell samples. , 1981, Cytometry.

[351]  C H Wang,et al.  Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles. , 1974, Biochemistry.

[352]  A. Glazer,et al.  Heterodimeric DNA-binding dyes designed for energy transfer: synthesis and spectroscopic properties. , 1993, Nucleic acids research.

[353]  M. Ratinaud,et al.  10-N nonyl-acridine orange: a fluorescent probe which stains mitochondria independently of their energetic state. , 1989, Biochemical and biophysical research communications.

[354]  A. B. Lyons,et al.  Divided we stand: Tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester , 1999, Immunology and cell biology.

[355]  D. Grunwald.  Flow cytometry and RNA studies , 1993, Biology of the cell.

[356]  T. Jovin,et al.  Fluorescence energy transfer measurements on cell surfaces: a critical comparison of steady-state fluorimetric and flow cytometric methods. , 1984, Cytometry.

[357]  F. S. Brackett,et al.  Determination of number and size of particles by electrical gating: blood cells. , 1957, Journal of applied physiology.

[358]  H. Tanke,et al.  Selection of defined cell types by flow-cytometric cell sorting. , 1993, Trends in biotechnology.

[359]  R. Tsien,et al.  A new generation of Ca2+ indicators with greatly improved fluorescence properties. , 1985, The Journal of biological chemistry.

[360]  R G Sweet,et al.  Fluorescence activated cell sorting. , 1972, The Review of scientific instruments.

[361]  Gary J. Weil,et al.  Eosinophil autofluorescence and its use in isolation and analysis of human eosinophils using flow microfluorometry. , 1981 .

[362]  J. Steinkamp,et al.  RAPID, SIMULTANEOUS MEASUREMENT OF DNA, PROTEIN, AND CELL VOLUME IN SINGLE CELLS FROM LARGE MAMMALIAN CELL POPULATIONS , 1973, The Journal of cell biology.

[363]  David Lloyd,et al.  Flow Cytometry in Microbiology , 1993, Springer London.

[364]  J A Steinkamp,et al.  Resolution of fluorescence signals from cells labeled with fluorochromes having different lifetimes by phase-sensitive flow cytometry. , 1993, Cytometry.

[365]  H. Gross,et al.  Detection of rare cells at a frequency of one per million by flow cytometry. , 1993, Cytometry.

[366]  T. Jovin,et al.  Proximity of lectin receptors on the cell surface measured by fluorescence energy transfer in a flow system. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[367]  R. Silver,et al.  Ratio imaging: practical considerations for measuring intracellular calcium and pH in living tissue. , 1998, Methods in cell biology.

[368]  G. Fink,et al.  Fluorescence activated cell sorting (FACS) as a separation method for neurofibrillary tangles in Alzheimer's disease , 1986, Journal of Neuroscience Methods.

[369]  Crissman Ha,et al.  Cell cycle-related changes in chromatin structure detected by flow cytometry using multiple DNA fluorochromes. , 1993 .

[370]  O. Wolfbeis,et al.  Syntheses of fluorescent dyes. XIV. Standards for fluorescence measurements in the near neutral pH-range† , 1982 .

[371]  J Kapuscinski,et al.  DAPI: a DNA-specific fluorescent probe. , 1995, Biotechnic & histochemistry : official publication of the Biological Stain Commission.

[372]  J. Leary,et al.  High‐Speed Flow Cytometric Analysis and Sorting of Human Fetal Cells from Maternal Blood for Molecular Characterization , 1994, Annals of the New York Academy of Sciences.

[373]  Bayard,et al.  Rapid hypotonic method for flow cytofluorometry of monolayer cell cultures. Some pitfalls in staining and data analysis. , 1978, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[374]  A Schwartz,et al.  Standardizing flow cytometry: a classification system of fluorescence standards used for flow cytometry. , 1998, Cytometry.

[375]  L. Johnson.  Advances in gender preselection in swine. , 2020, Journal of reproduction and fertility. Supplement.

[376]  J A Aten,et al.  Slit-scanning technique using standard cell sorter instruments for analyzing and sorting nonacrocentric human chromosomes, including small ones. , 1994, Cytometry.

[377]  N. Keiding,et al.  Long-term storage of samples for flow cytometric DNA analysis. , 1983, Cytometry.

[378]  M. Nüsse,et al.  Light scatter of isolated cell nuclei as a parameter discriminating the cell-cycle subcompartments. , 1994, Methods in cell biology.

[379]  D. Gerson,et al.  Intracellular pH of mitogen-stimulated lymphocytes. , 1982, Science.

[380]  J. Monroe,et al.  Enrichment of viable lymphocytes in defined cycle phases by sorting on the basis of pulse width of axial light extinction. , 1982, Cytometry.

[381]  J A Steinkamp,et al.  Correlated measurements of DNA, RNA, and protein in individual cells by flow cytometry. , 1985, Science.

[382]  H. Gratzner,et al.  Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. , 1982, Science.

[383]  R. Tsien,et al.  Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. , 1989, The Journal of biological chemistry.

[384]  S. Damjanovich,et al.  Physical association between MHC class I and class II molecules detected on the cell surface by flow cytometric energy transfer. , 1989, Journal of immunology.

[385]  D. K. Green,et al.  Purifying human Y chromosomes by flow cytometry and sorting. , 1983, Cytometry.

[386]  Richard P. Haugland,et al.  Handbook of fluorescent probes and research chemicals , 1996 .

[387]  S. F. Schlossman,et al.  CD antigens 1993. , 1994, Immunology today.