Spliceosomal RNA U6 is remarkably conserved from yeast to mammals

The small nuclear RNA U6 and its gene have been isolated from yeast. In striking contrast to other yeast spliceosomal RNAs, U6 is very similar in size, sequence and structure to its mammalian homologue. The single-copy gene is essential. These properties suggest a central role in pre-mRNA processing. An extensive base-pairing interaction with U4 snRNA is described; the destabilization of the U4/U6 complex seen during splicing thus requires a large conformational change.

[1]  H. Busch,et al.  Small nuclear RNAs and RNA processing. , 1983, Progress in nucleic acid research and molecular biology.

[2]  R. Lührmann,et al.  Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing. , 1984, The EMBO journal.

[3]  P. Sharp,et al.  Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[4]  R. Maser,et al.  U6 small nuclear RNA is transcribed by RNA polymerase III. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[5]  C. Guthrie,et al.  Saccharomyces cerevisiae has a U1-like small nuclear RNA with unexpected properties. , 1987, Science.

[6]  M. Rose.  [53] Isolation of genes by complementation in yeast , 1987 .

[7]  Tom Maniatis,et al.  The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing , 1987, Nature.

[8]  M. Rosbash,et al.  S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA , 1987, Cell.

[9]  T. Pederson,et al.  Upstream elements required for efficient transcription of a human U6 RNA gene resemble those of U1 and U2 genes even though a different polymerase is used. , 1988, Genes & development.

[10]  U. Pettersson,et al.  A distant enhancer element is required for polymerase III transcription of a U6 RNA gene , 1987, Nature.

[11]  D. Wright,et al.  The capped U6 small nuclear RNA is transcribed by RNA polymerase III. , 1987, The Journal of biological chemistry.

[12]  F. Solymosy,et al.  Plant small nuclear RNAs. II. U6 RNA and a 4.5SI-like RNA are present in plant nuclei. , 1987, Nucleic acids research.

[13]  R. Reddy,et al.  Structure, organization, and transcription of Drosophila U6 small nuclear RNA genes. , 1987, The Journal of biological chemistry.

[14]  D. Wright,et al.  Upstream regulatory elements are necessary and sufficient for transcription of a U6 RNA gene by RNA polymerase III. , 1988, The EMBO journal.

[15]  C. Branlant,et al.  Primary and secondary structures of chicken, rat and man nuclear U4 RNAs. Homologies with U1 and U5 RNAs. , 1981, Nucleic acids research.

[16]  C. Guthrie,et al.  An essential yeast snRNA with a U5-like domain is required for splicing in vivo , 1987, Cell.

[17]  J. Steitz,et al.  Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein , 1986, Cell.

[18]  J. Steitz,et al.  U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle. , 1984, Nucleic acids research.

[19]  M. Rosbash,et al.  Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes , 1986, Nature.

[20]  J W Szostak,et al.  Yeast transformation: a model system for the study of recombination. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[21]  M. Melli,et al.  The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter , 1987, Cell.

[22]  S. Cheng,et al.  Spliceosome assembly in yeast. , 1987, Genes & development.

[23]  R. Lührmann,et al.  Localization of a base-paired interaction between small nuclear RNAs U4 and U6 in intact U4/U6 ribonucleoprotein particles by psoralen cross-linking. , 1985, Journal of molecular biology.

[24]  S. Cheng,et al.  Fractionation and characterization of a yeast mRNA splicing extract. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[25]  P. Carbon,et al.  Xenopus tropicalis U6 snRNA genes transcribed by Pol III contain the upstream promoter elements used by Pol II dependent U snRNA genes. , 1987, Nucleic acids research.

[26]  W. Gilbert,et al.  Mapping adenines, guanines, and pyrimidines in RNA. , 1977, Nucleic acids research.

[27]  D. Turner,et al.  Improved free-energy parameters for predictions of RNA duplex stability. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[28]  K. Murata,et al.  Transformation of intact yeast cells treated with alkali cations , 1983 .

[29]  G. Natsoulis,et al.  5-Fluoroorotic acid as a selective agent in yeast molecular genetics. , 1987, Methods in enzymology.

[30]  E. Geiduschek,et al.  Modulation of yeast 5 S rRNA synthesis in vitro by ribosomal protein YL3. A possible regulatory loop. , 1987, The Journal of biological chemistry.

[31]  S. Nishimura,et al.  The nucleotide sequence of nuclear 4.8S RNA of mouse cells. , 1980, Biochemical and biophysical research communications.

[32]  D. S. Adams,et al.  Nucleotide sequence of Physarum U6 small RNA , 1987, Nucleic Acids Res..

[33]  M. Ares.  U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs , 1986, Cell.

[34]  Y. Ohshima,et al.  Nucleotide sequences of mouse genomic loci including a gene or pseudogene for U6 (4.8S) nuclear RNA. , 1981, Nucleic acids research.

[35]  J. Broach.  [21] Construction of high copy yeast vectors using 2-μm circle sequences , 1983 .

[36]  H. Busch,et al.  The primary nucleotide sequence of U4 RNA. , 1981, The Journal of biological chemistry.

[37]  C. Guthrie,et al.  A subset of yeast snRNA's contains functional binding sites for the highly conserved Sm antigen. , 1987, Science.

[38]  R. Roeder,et al.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. , 1983, Nucleic acids research.

[39]  G. Stormo,et al.  Translational regulation of expression of the bacteriophage T4 lysozyme gene. , 1986, Nucleic acids research.

[40]  O. Uhlenbeck,et al.  Specific labeling of 3' termini of RNA with T4 RNA ligase. , 1980, Methods in enzymology.

[41]  C. Guthrie,et al.  An essential snRNA from S. cerevisiae has properties predicted for U4, including interaction with a U6-like snRNA , 1987, Cell.

[42]  P. Carbon,et al.  A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II , 1987, Cell.

[43]  F. Harada,et al.  Nucleotide sequence of nuclear 5.7S RNA of mouse cells. , 1981, Biochemical and biophysical research communications.

[44]  Uttam L. RajBhandary,et al.  Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis , 1978, Nucleic Acids Res..