Although nanofibrous meshes are considered promising cultivation beds for maintaining cell differentiation, three-dimensional (3D) cultivation is not possible because their nanoporous structures impede cell infiltration. To facilitate transverse cell migration across nanofibrous meshes, we prepared electrospun nanofibers with structures that varied in response to red laser light. Polyoxalate (POX), composed of oxalate linkers and oligomeric caprolactone, was synthesized and electrospun into fibrous meshes with a photosensitizer (chlorin e6: Ce6). These meshes exhibited morphological and chemical changes upon laser irradiation, and mass erosion rates of the meshes were faster after laser irradiation. Cell cultivation on POX meshes revealed that red laser effectively facilitated traverse migration of the cells without affecting cell viability. We envision the use of light-triggered change of meshes to promote the migration of cells during 3D matrix cultivation. This article is protected by copyright. All rights reserved.