Multi-allergen Quantitation and the Impact of Thermal Treatment in Industry-Processed Baked Goods by ELISA and Liquid Chromatography-Tandem Mass Spectrometry.

Undeclared food allergens account for 30-40% of food recalls in the United States. Compliance with ingredient labeling regulations and the implementation of effective manufacturing allergen control plans require the use of reliable methods for allergen detection and quantitation in complex food products. The objectives of this work were to (1) produce industry-processed model foods incurred with egg, milk, and peanut allergens, (2) compare analytical method performance for allergen quantitation in thermally processed bakery products, and (3) determine the effects of thermal treatment on allergen detection. Control and allergen-incurred cereal bars and muffins were formulated in a pilot-scale industry processing facility. Quantitation of egg, milk, and peanut in incurred baked goods was compared at various processing stages using commercial enzyme-linked immunosorbent assay (ELISA) kits and a novel multi-allergen liquid chromatography (LC)-tandem mass spectrometry (MS/MS) multiple-reaction monitoring (MRM) method. Thermal processing was determined to negatively affect the recovery and quantitation of egg, milk, and peanut to different extents depending on the allergen, matrix, and analytical test method. The Morinaga ELISA and LC-MS/MS quantitative methods reported the highest recovery across all monitored allergens, whereas the ELISA Systems, Neogen BioKits, Neogen Veratox, and R-Biopharm ELISA Kits underperformed in the determination of allergen content of industry-processed bakery products.

[1]  J. Ring,et al.  Lysozyme in wine: A risk evaluation for consumers allergic to hen's egg. , 2009, Molecular nutrition & food research.

[2]  T. Matsuda,et al.  Decrease in antigenic and allergenic potentials of ovomucoid by heating in the presence of wheat flour: dependence on wheat variety and intermolecular disulfide bridges. , 2001, Journal of agricultural and food chemistry.

[3]  T. Whitaker,et al.  Immunochemical analytical methods for the determination of peanut proteins in foods. , 2005, Journal of AOAC International.

[4]  Philip R Goodwin,et al.  Food allergen detection methods: a coordinated approach. , 2004, Journal of AOAC International.

[5]  W. Nowatzke,et al.  Multiplex detection of food allergens and gluten , 2015, Analytical and Bioanalytical Chemistry.

[6]  Maria Careri,et al.  A Rapid Size-Exclusion Solid-Phase Extraction Step for Enhanced Sensitivity in Multi-Allergen Determination in Dark Chocolate and Biscuits by Liquid Chromatography–Tandem Mass Spectrometry , 2013, Food Analytical Methods.

[7]  I. Giovannacci,et al.  13 – Detecting dairy and egg residues in food , 2006 .

[8]  T. Whitaker,et al.  Effect of processing on recovery and variability associated with immunochemical analytical methods for multiple allergens in a single matrix: dark chocolate. , 2012, Journal of agricultural and food chemistry.

[9]  J. Callahan,et al.  Size-selective fractionation and visual mapping of allergen protein chemistry in Arachis hypogaea. , 2012, Journal of proteome research.

[10]  Linda Monaci,et al.  Influence of baking time and matrix effects on the detection of milk allergens in cookie model food system by ELISA. , 2011, Food chemistry.

[11]  T. Matsuda,et al.  Antigenicity of Ovomucoid Remaining in Boiled Shell Eggs , 1986 .

[12]  E. Anklam,et al.  Inter-laboratory validation study of five commercial ELISA test kits for the determination of peanut proteins in biscuits and dark chocolate , 2005, Food additives and contaminants.

[13]  S. Muraoka,et al.  Novel ELISA for the detection of raw and processed egg using extraction buffer containing a surfactant and a reducing agent. , 2005, Journal of immunological methods.

[14]  E. Knol,et al.  Purification and immunoglobulin E‐binding properties of peanut allergen Ara h 6: evidence for cross‐reactivity with Ara h 2 , 2005, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

[15]  Jerry Zweigenbaum,et al.  Discovery of highly conserved unique peanut and tree nut peptides by LC-MS/MS for multi-allergen detection. , 2016, Food chemistry.

[16]  Y. Mine,et al.  Comparative studies on antigenicity and allergenicity of native and denatured egg white proteins. , 2002, Journal of agricultural and food chemistry.

[17]  J. Wal.  Thermal processing and allergenicity of foods , 2003, Allergy.

[18]  Rosa Pilolli,et al.  Multi-allergen detection in food by micro high-performance liquid chromatography coupled to a dual cell linear ion trap mass spectrometry. , 2014, Journal of chromatography. A.

[19]  Katie Banaszewski,et al.  Effect of heat treatment on the quantitative detection of egg protein residues by commercial enzyme-linked immunosorbent assay test kits. , 2010, Journal of agricultural and food chemistry.

[20]  B. Blais,et al.  Multiplex enzyme immunoassay system for the simultaneous detection of multiple allergens in foods , 2003 .

[21]  Ruedi Aebersold,et al.  Options and considerations when selecting a quantitative proteomics strategy , 2010, Nature Biotechnology.

[22]  J. Heick,et al.  First screening method for the simultaneous detection of seven allergens by liquid chromatography mass spectrometry. , 2011, Journal of chromatography. A.

[23]  H. Narita,et al.  Recognition of Native and/or Thermally Induced Denatured Forms of the Major Food Allergen, Ovomucoid, by Human IgE and Mouse Monoclonal IgG Antibodies , 2004, Bioscience, biotechnology, and biochemistry.

[24]  P. Scholl,et al.  Quantification of allergenic bovine milk α(S1)-casein in baked goods using an intact ¹⁵N-labeled protein internal standard. , 2013, Journal of agricultural and food chemistry.

[25]  S Vieths,et al.  Indirect competitive ELISA for determination of traces of peanut (Arachis hypogaea L.) protein in complex food matrices. , 1999, Journal of agricultural and food chemistry.

[26]  Karluss Thomas,et al.  Evaluating the effect of food processing on the potential human allergenicity of novel proteins: international workshop report. , 2007, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[27]  Daniel B. Martin,et al.  Computational prediction of proteotypic peptides for quantitative proteomics , 2007, Nature Biotechnology.

[28]  Rosa Pilolli,et al.  Orbitrap™ monostage MS versus hybrid linear ion trap MS: application to multi-allergen screening in wine. , 2014, Journal of mass spectrometry : JMS.

[29]  B. Wüthrich,et al.  Comparison of commercially available ELISA kits with human sera-based detection methods for peanut allergens in foods , 2003, Food additives and contaminants.

[30]  K. E. Løvberg,et al.  Extractability, stability, and allergenicity of egg white proteins in differently heat-processed foods. , 2007, Journal of AOAC International.

[31]  Brendan MacLean,et al.  Bioinformatics Applications Note Gene Expression Skyline: an Open Source Document Editor for Creating and Analyzing Targeted Proteomics Experiments , 2022 .

[32]  W. Dolen,et al.  The Prevalence, Severity, and Distribution of Childhood Food Allergy in the United States , 2012, Pediatrics.

[33]  M. Kulis,et al.  The 2S albumin allergens of Arachis hypogaea, Ara h 2 and Ara h 6, are the major elicitors of anaphylaxis and can effectively desensitize peanut‐allergic mice , 2012, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

[34]  Richard B Raybourne,et al.  Evaluation of extraction buffers using the current approach of detecting multiple allergenic and nonallergenic proteins in food. , 2004, Journal of AOAC International.

[35]  Elke Anklam,et al.  Effects of chemical, physical, and technological processes on the nature of food allergens. , 2004, Journal of AOAC International.

[36]  E. Anklam,et al.  Effect of roasting history and buffer composition on peanut protein extraction efficiency. , 2004, Molecular nutrition & food research.

[37]  L. Deterding,et al.  Identification of Maillard reaction products on peanut allergens that influence binding to the receptor for advanced glycation end products , 2013, Allergy.

[38]  T. Whitaker,et al.  Effect of processing on recovery and variability associated with immunochemical analytical methods for multiple allergens in a single matrix: sugar cookies. , 2012, Journal of agricultural and food chemistry.

[39]  Tong-Jen Fu,et al.  Impact of thermal processing on ELISA detection of peanut allergens. , 2013, Journal of agricultural and food chemistry.

[40]  S. Dreskin,et al.  Ara h 2 and Ara h 6 Have Similar Allergenic Activity and Are Substantially Redundant , 2012, International Archives of Allergy and Immunology.

[41]  Tong-Jen Fu,et al.  Effects of processing on the recovery of food allergens from a model dark chocolate matrix. , 2015, Food chemistry.

[42]  H. D. de Jongh,et al.  Heat-induced Conformational Changes of Ara h 1, a Major Peanut Allergen, Do Not Affect Its Allergenic Properties* , 1999, The Journal of Biological Chemistry.

[43]  R. Pieters,et al.  Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens. , 2015, Journal of agricultural and food chemistry.

[44]  J. Garin,et al.  Isotope dilution strategies for absolute quantitative proteomics. , 2009, Journal of proteomics.

[45]  A. Paschke-Kratzin,et al.  Comparison of extraction conditions for milk and hen's egg allergens , 2011, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[46]  Scott H Sicherer,et al.  Clinical reviews in allergy and immunology , 2022 .

[47]  T. Yasaki,et al.  Allergenic activity of heated and ovomucoid-depleted egg white. , 1997, The Journal of allergy and clinical immunology.

[48]  Willem Haasnoot,et al.  Luminex-based triplex immunoassay for the simultaneous detection of soy, pea, and soluble wheat proteins in milk powder. , 2007, Journal of agricultural and food chemistry.

[49]  M. Pischetsrieder,et al.  Modified peptides as indicators for thermal and nonthermal reactions in processed milk. , 2014, Journal of agricultural and food chemistry.

[50]  J. Callahan,et al.  Global proteomic screening of protein allergens and advanced glycation endproducts in thermally processed peanuts. , 2013, Journal of agricultural and food chemistry.

[51]  S. Vieths,et al.  Structure and stability of 2S albumin-type peanut allergens: implications for the severity of peanut allergic reactions. , 2006, The Biochemical journal.

[52]  R. Marchelli,et al.  The effect of heat treatment on the detection of peanut allergens as determined by ELISA and real-time PCR , 2009, Analytical and bioanalytical chemistry.

[53]  R. Aebersold,et al.  Selected reaction monitoring for quantitative proteomics: a tutorial , 2008, Molecular systems biology.

[54]  S. Gendel,et al.  Analysis of U.S. Food and Drug Administration food allergen recalls after implementation of the food allergen labeling and consumer protection act. , 2013, Journal of food protection.

[55]  E. Knol,et al.  Quantification of major peanut allergens Ara h 1 and
Ara h 2 in the peanut varieties Runner, Spanish, Virginia,
and Valencia, bred in different parts of the world , 2001, Allergy.

[56]  F. E. Cunningham,et al.  Egg-white lysozyme as a food preservative: an overview , 1991 .

[57]  M. Mann,et al.  Universal sample preparation method for proteome analysis , 2009, Nature Methods.

[58]  Anna Nowak-Wegrzyn,et al.  Rare, medium, or well done? The effect of heating and food matrix on food protein allergenicity , 2009, Current opinion in allergy and clinical immunology.

[59]  Ahmed Gomaa,et al.  Simultaneous detection of multi-allergens in an incurred food matrix using ELISA, multiplex flow cytometry and liquid chromatography mass spectrometry (LC-MS). , 2015, Food chemistry.

[60]  Ying Chen,et al.  Simultaneous detection of eight food allergens using optical thin-film biosensor chips. , 2011, Journal of agricultural and food chemistry.