Characterization of human papillomavirus type 45, a new type 18-related virus of the genital tract.

DNA of human papillomavirus (HPV) type 45, a new HPV type 18-related papillomavirus of the genital tract, was cloned from a recurrent cervical lesion displaying mild to moderate dysplasia with koilocytosis. HPV-45 DNA was identified in paraffin sections of biopsies of both the initial and recurrent lesions of the patient, taken 7 months apart. HPV-45 DNA hybridized efficiently to that of many different HPV types under low and moderate stringency conditions (Tm - 37 degrees C to Tm - 25 degrees C) but with only HPV-18 DNA under high stringency conditions (Tm - 17 degrees C). HPV-45 DNA was distinguished from HPV-18 DNA by (i) differences in restriction enzyme digest patterns, (ii) lack of hybridization at Tm - 17 degrees C between HPV-18 and some fragments of HPV-45, (iii) a value of 25% in liquid reassociation kinetics between HPV-18 and HPV-45 and (iv) differences in intensities of hybridization with selected tissue DNAs. The prevalence of HPV-45 infection in the genital tract was low. In tests of over 600 tissue DNAs from female genital tract lesions, HPV-45 sequences were detected in three additional tissues, one each of invasive cervical carcinoma, condyloma, and normal cervical epithelium. HPV-45 is a newly recognized papillomavirus which rarely infects the genital tract and is associated with lesions across a wide histological spectrum.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  K. Shah,et al.  Multicentric Human Papillomavirus Infections of the Female Genital Tract: Correlation of Viral Types With Abnormal Mitotic Figures, Colposcopic Presentation, and Location , 1987, Obstetrics and gynecology.

[3]  G. Temple,et al.  Correlation of Cellular Atypia and Human Papillomavirus Deoxyribonucleic Acid Sequences in Exfoliated Cells of the Uterine Cervix , 1986, Obstetrics and gynecology.

[4]  H. Kashima,et al.  Identification of genital tract papillomaviruses HPV‐6 and HPV‐16 in warts of the oral cavity , 1985, Journal of medical virology.

[5]  K. Shah,et al.  Specific Identification of Human Papillomavirus Type in Cervical Smears and Paraffin Sections by In Situ Hybridization with Radioactive Probes: A Preliminary Communication , 1985, International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists.

[6]  L. Gissmann,et al.  The physical state of human papillomavirus type 16 DNA in benign and malignant genital tumours. , 1985, The Journal of general virology.

[7]  Wolfgang Mayer,et al.  Structure and transcription of human papillomavirus sequences in cervical carcinoma cells , 1985, Nature.

[8]  D. Melton,et al.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. , 1984, Nucleic acids research.

[9]  H. Hausen,et al.  A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. , 1984, The EMBO journal.

[10]  K. Shah,et al.  Distribution of Human Papillomavirus Antigen in Cervicovaginal Smears and Cervical Tissues , 1983, International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists.

[11]  V. Diehl,et al.  Molecular cloning and characterization of human papilloma virus DNA derived from a laryngeal papilloma , 1982, Journal of virology.

[12]  W. J. Brammar,et al.  A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. , 1980, Gene.

[13]  B. McConaughy,et al.  Nucleic acid reassociation in formamide. , 1969, Biochemistry.

[14]  N. Davidson,et al.  Kinetics of renaturation of DNA. , 1968, Journal of molecular biology.

[15]  D T Denhardt,et al.  A membrane-filter technique for the detection of complementary DNA. , 1966, Biochemical and biophysical research communications.

[16]  G. Temple,et al.  Sexually transmitted papillomaviral infections. I. The anatomic distribution and pathologic grade of neoplastic lesions associated with different viral types. , 1987, American journal of obstetrics and gynecology.