The Use Of Digital Image Processing Techniques For The Calibration Of Quantitative Microscopes

In the construction of several new quantitative microscope systems we have developed techniques for the measurement and calibration of spatial resolution, image sampling densi-ty, signal-to-noise ratio, photometric response, and shading. The approach makes use of several commercially available test specimens, a test slide that was specially developed, and a number of algorithms to provide calibration of the complete system from the input specimen to the stored digital image including optical, electro-optical, analog, and digital sources of signal degradation.