Functional validation of co‐culture model of human keratinocytes and neuronal cell line for sensitive skin by using transient receptor potential channel vanilloid subfamily member 1 antagonist

Sensitive skin is a subjective cutaneous hyper‐reactivity that occurs in response to various innocuous stimuli. Keratinocytes have recently been shown to participate in sensory transduction by releasing many neuroactive molecules that bind to intra‐epidermal free nerve endings and modulate nociception. In the literature, the characterization of these interactions has been based on the co‐culture of keratinocyte and mammalian‐origin neuronal cell lines. In this study, we established an in vitro model based on a co‐culture of primary human keratinocytes and differentiated SH‐SY5Y cells, a human neuronal cell line.

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