Esophageal cancer detection based on tissue surface-enhanced Raman spectroscopy and multivariate analysis

The capability of using silver nanoparticle based near-infrared surface enhanced Raman scattering (SERS) spectroscopy combined with principal component analysis (PCA) and linear discriminate analysis (LDA) to differentiate esophageal cancer tissue from normal tissue was presented. Significant differences in Raman intensities of prominent SERS bands were observed between normal and cancer tissues. PCA-LDA multivariate analysis of the measured tissue SERS spectra achieved diagnostic sensitivity of 90.9% and specificity of 97.8%. This exploratory study demonstrated great potential for developing label-free tissue SERS analysis into a clinical tool for esophageal cancer detection.

[1]  Zufang Huang,et al.  Nasopharyngeal cancer detection based on blood plasma surface-enhanced Raman spectroscopy and multivariate analysis. , 2010, Biosensors & bioelectronics.

[2]  Bernhard Lendl,et al.  A New Method for Fast Preparation of Highly Surface-Enhanced Raman Scattering (SERS) Active Silver Colloids at Room Temperature by Reduction of Silver Nitrate with Hydroxylamine Hydrochloride , 2003 .

[3]  Sebastian Wachsmann-Hogiu,et al.  Chemical analysis in vivo and in vitro by Raman spectroscopy--from single cells to humans. , 2009, Current opinion in biotechnology.

[4]  Kenji Kobayashi,et al.  Endoscopic screening of early esophageal cancer with the Lugol dye method in patients with head and neck cancers , 1990, Cancer.

[5]  S. Lam,et al.  Near‐infrared Raman spectroscopy for optical diagnosis of lung cancer , 2003, International journal of cancer.

[6]  Rong Chen,et al.  Label-free serum ribonucleic acid analysis for colorectal cancer detection by surface-enhanced Raman spectroscopy and multivariate analysis. , 2012, Journal of biomedical optics.

[7]  K. Kneipp,et al.  SERS--a single-molecule and nanoscale tool for bioanalytics. , 2008, Chemical Society reviews.

[8]  Rong Chen,et al.  Gastric cancer detection based on blood plasma surface-enhanced Raman spectroscopy excited by polarized laser light. , 2011, Biosensors & bioelectronics.

[9]  M. Altaş,et al.  Differentiation of Healthy Brain Tissue and Tumors Using Surface-Enhanced Raman Scattering , 2009, Applied spectroscopy.

[10]  S. Aștilean,et al.  Bridging biomolecules with nanoparticles: surface‐enhanced Raman scattering from colon carcinoma and normal tissue , 2008 .

[11]  S. Lane,et al.  Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells. , 2006, Biophysical journal.

[12]  M. Fleischmann,et al.  Raman spectra of pyridine adsorbed at a silver electrode , 1974 .

[13]  Zufang Huang,et al.  Gold Nanoparticle Based Surface-Enhanced Raman Scattering Spectroscopy of Cancerous and Normal Nasopharyngeal Tissues under Near-Infrared Laser Excitation , 2009, Applied spectroscopy.

[14]  Mads S. Bergholt,et al.  In Vivo Diagnosis of Esophageal Cancer Using Image-Guided Raman Endoscopy and Biomolecular Modeling , 2011, Technology in cancer research & treatment.

[15]  Yanhua Tong,et al.  Silver vanadate nanoribbons: A label-free bioindicator in the conversion between human serum transferrin and apotransferrin via surface-enhanced Raman scattering , 2011 .

[16]  Luke P. Lee,et al.  Nanowell surface enhanced Raman scattering arrays fabricated by soft-lithography for label-free biomolecular detections in integrated microfluidics , 2005 .

[17]  Yanlin Song,et al.  Enhanced nanoparticle-oligonucleotide conjugates for DNA nanomachine controlled surface-enhanced Raman scattering switch , 2011 .

[18]  D. McLean,et al.  Automated Autofluorescence Background Subtraction Algorithm for Biomedical Raman Spectroscopy , 2007, Applied spectroscopy.