Bio-electrosprays: a novel electrified jetting methodology for the safe handling and deployment of primary living organisms.

Electrohydrodynamic jetting (EHDJ) which is also known as electrosprays (ES) has recently been elucidated as a unique electrified biotechnique for the safe handling and deployment of living organisms. This high intensity electric field driven jetting methodology is now referred to as "bioelectrosprays" (BES). Previously these charged jets have only been shown to jet-process immortalized cells which have undergone expected cellular behavior when compared with control cells. In this paper we demonstrate the ability to jet process primary living organisms in the stable conejetting mode. Finally the viability of the bio-electrosprayed living organisms has been assessed employing a flow cytometry approach which forms the discussion in this paper. Our findings further establish BES as a competing biotechnique, which could be employed for the deposition of primary living organisms according to a predetermined active cellular architecture. One day this could be used for the fabrication of viable tissues and organs for repair or replacement. These advanced studies carried out on BES have direct widespread applications ranging from developmental biology to regenerative and therapeutic medicine, which are a few amongst several other areas of study within the life sciences.

[1]  D. J. Brunner,et al.  ELECTROHYDRODYNAMIC ATOMIZATION IN THE CONE–JET MODE PHYSICAL MODELING OF THE LIQUID CONE AND JET , 1997 .

[2]  M. Mann,et al.  Electrospray ionization for mass spectrometry of large biomolecules. , 1989, Science.

[3]  W. Balachandran,et al.  Electrospray of fine droplets of ceramic suspensions for thin-film preparation , 2001 .

[4]  Suwan N Jayasinghe,et al.  Bio-electrosprays: the next generation of electrified jets. , 2006, Biotechnology journal.

[5]  A. Jaworek,et al.  Classification of the Modes of Ehd Spraying , 1999 .

[6]  Geoffrey Ingram Taylor,et al.  Disintegration of water drops in an electric field , 1964, Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences.

[7]  M. Edirisinghe,et al.  Effect of viscosity on the size of relics produced by electrostatic atomization , 2002 .

[8]  A. Gañán-Calvo,et al.  THE ROLE OF THE ELECTRICAL CONDUCTIVITY AND VISCOSITY ON THE MOTIONS INSIDE TAYLOR CONES , 1999 .

[9]  C Haanen,et al.  A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. , 1995, Journal of immunological methods.

[10]  T. Czech,et al.  Electrostatic interaction of free EHD jets , 2006 .

[11]  John Zeleny,et al.  Instability of Electrified Liquid Surfaces , 1917 .

[12]  R. Flagan,et al.  Electrospray Atomization of Electrolytic Solutions , 1994 .

[13]  A Townsend-Nicholson,et al.  Stable electric-field driven cone-jetting of concentrated biosuspensions. , 2006, Lab on a chip.

[14]  Alfonso M. Gañán-Calvo,et al.  Cone-Jet Analytical Extension of Taylor's Electrostatic Solution and the Asymptotic Universal Scaling Laws in Electrospraying , 1997 .

[15]  S. Jayasinghe.  Self-assembled nanostructures via electrospraying , 2006 .

[16]  M. Edirisinghe,et al.  Relic and droplet sizes produced by electrostatic atomisation of ceramic suspensions , 2004 .

[17]  I. Hayati,et al.  Mechanism of stable jet formation in electrohydrodynamic atomization , 1986, Nature.

[18]  J. Rosell-Llompart,et al.  Generation of monodisperse droplets 0.3 to 4 μm in diameter from electrified cone-jets of highly conducting and viscous liquids , 1994 .

[19]  John R. Albright,et al.  Mass dependence of the momentum-transfer distributions in pi+ p ---> p 8pi at 11.0 gev/c , 1974 .

[20]  A. Gomez,et al.  Monodisperse Electrosprays of Low Electric Conductivity Liquids in the Cone-Jet Mode , 1996, Journal of colloid and interface science.

[21]  S. Jayasinghe.  A Novel Technique for Forming Self‐Assembled Nanotube Structures , 2006 .

[22]  T. Pearson,et al.  The clonal characteristics of human aortic intima. Comparison with fatty streaks and normal media. , 1983, The American journal of pathology.

[23]  M. Cloupeau,et al.  ELECTROHYDRODYNAMIC SPRAYING FUNCTIONING MODES - A CRITICAL-REVIEW , 1994 .

[24]  D. J. Brunner,et al.  The evolution of electrohydrodynamic sprays produced in the cone-jet mode, a physical model , 1999 .

[25]  M. Márquez,et al.  Micro/Nano Encapsulation via Electrified Coaxial Liquid Jets , 2002, Science.

[26]  Da-Ren Chen,et al.  A Novel Approach for Introducing Bio-Materials Into Cells , 2000 .

[27]  Suwan N. Jayasinghe,et al.  An advanced jet-based approach to processing nanotubes , 2006 .

[28]  L. Rayleigh.  On the Capillary Phenomena of Jets , 1879 .

[29]  Jan Pieter Abrahams,et al.  Nano-dispensing by electrospray for biotechnology. , 2006, Biotechnology journal.

[30]  D. J. Brunner,et al.  JET BREAK-UP IN ELECTROHYDRODYNAMIC ATOMIZATION IN THE CONE-JET MODE , 2000 .

[31]  Dazhi Wang,et al.  Instrument for electrohydrodynamic print-patterning three-dimensional complex structures , 2005 .

[32]  Geunhyung Kim,et al.  Production of microsized PMMA droplets using electrospraying with various auxiliary fields. , 2006, Journal of colloid and interface science.

[33]  Suwan N Jayasinghe,et al.  Electrohydrodynamic jet processing: an advanced electric-field-driven jetting phenomenon for processing living cells. , 2006, Small.

[34]  Suwan N Jayasinghe,et al.  Electric field driven jetting: an emerging approach for processing living cells , 2006, Biotechnology journal.