论文信息 - Isolation and characterization of polymorphic microsatellite loci in nurse shark (Ginglymostoma cirratum)

Isolation and characterization of polymorphic microsatellite loci in nurse shark (Ginglymostoma cirratum)

We report on the development of nine polymorphic microsatellite loci in nurse shark ( Ginglymostoma cirratum ) using a combination of enriched and unenriched subgenomic libraries. Based on the small percentage of positive clones in the unenriched library (0.4%) it appears that microsatellites are very scarce in nurse shark genomes. Numbers of alleles at polymorphic loci ranged from two to 15; observed heterozygosity ranged from 0.17 to 0.90. We expect these loci to be useful for studies of breeding structure and paternity.

[1] H. L. Pratt,et al. Multiple Paternity in the Nurse Shark, Ginglymostoma Cirratum , 2002, Environmental Biology of Fishes.

[2] S. Gruber,et al. Multiple Paternity of a Lemon Shark Litter (Chondrichthyes: Carcharhinidae) , 2001, Copeia.

[3] H. L. Pratt,et al. A Review of Elasmobranch Reproductive Behavior with a Case Study on the Nurse Shark, Ginglymostoma Cirratum , 2001, Environmental Biology of Fishes.

[4] M. Flajnik,et al. Primitive synteny of vertebrate major histocompatibility complex class I and class II genes. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[5] A. Di Fiore,et al. Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. , 1999, BioTechniques.

[6] J. Gold,et al. MICROSATELLITE DNA VARIATION IN SANDBAR SHARKS (CARCHARHINUS PLUMBEUS) FROM THE GULF OF MEXICO AND MID-ATLANTIC BIGHT , 1999 .

[7] François Rousset,et al. GENEPOP (version 1.2): population genetic software for exact tests and ecumenicism , 1995 .

[8] H. L. Pratt,et al. Group reproductive behaviors in free-living nurse sharks, Ginglymostoma cirratum , 1994 .

[9] C. Luer,et al. Growth rates in the nurse shaark, Ginglymostoma cirratum , 1990 .