Nonmechanically Agitated Bioreactors

Bubble column and airlift reactors are normally the first choice in many applications, especially ones involving biomaterials. Their popularity is essentially related to the simple design, nonmechanical agitation, and satisfactory heat and mass transfer properties. However, problems related to poor mixing, scale-up, product quality, and process reproducibility are typically reported. To overcome some of these limitations, oscillatory flow reactors (OFR) have been studied. These reactors, located on the frontier of mechanically and nonmechanically agitator reactors, will be explored in this chapter. The following sections present the two most common reactors (bubble column and airlift) used in bioprocesses as well as OFRs, highlighting the advantages and limitations of these reactors and their possible contribution to future developments in biotechnology and bioengineering.

[1]  X. Ni,et al.  Effect of hydrodynamics on mass transfer in a gas–liquid oscillatory baffled column , 2004 .

[2]  Y. Chisti,et al.  Shear rate in stirred tank and bubble column bioreactors , 2006 .

[3]  X. Ni,et al.  Characterization of a gas‐liquid OBC: Bubble size and gas holdup , 2004 .

[4]  António A. Vicente,et al.  Hydrodynamic considerations on optimal design of a three‐phase airlift bioreactor with high solids loading , 2003 .

[5]  A. Schumpe,et al.  Gas-liquid interfacial areas in a bubble column with suspended solids , 1984 .

[6]  Effect of bubble column dimensions on flow regime transition , 2001 .

[7]  Robert W. Field,et al.  Bubble Column Reactors , 1991 .

[8]  Jorge M. T. Vasconcelos,et al.  Effect of bubble contamination on rise velocity and mass transfer , 2005 .

[9]  Aniruddha B. Pandit,et al.  Enhancement of gas-liquid mass transfer using oscillatory flow in a baffled tube , 1993 .

[10]  Hongbing Jian,et al.  A Numerical Study on the Scale-Up Behaviour in Oscillatory Baffled Columns , 2005 .

[11]  M. Pons,et al.  Using image analysis in the study of multiphase gas absorption , 2005 .

[12]  C. Cooney,et al.  Bioreactors, Continuous Stirred‐Tank Reactors , 2002 .

[13]  Anh N. Phan,et al.  Development and evaluation of novel designs of continuous mesoscale oscillatory baffled reactors , 2010 .

[14]  N. H. Thomas,et al.  Homogeneous–heterogeneous regime transition in bubble columns , 2001 .

[15]  Nobuya Fujiwara,et al.  Gas-liquid mass transfer characteristics in a bubble column with suspended sparingly soluble fine particles , 1985 .

[16]  Malcolm R. Mackley,et al.  The mixing and separation of particle suspensions using oscillatory flow in baffled tubes , 1993 .

[17]  Adam Harvey,et al.  Fluid Mechanics and Design Aspects of a Novel Oscillatory Flow Screening Mesoreactor , 2005 .

[18]  Malcolm R. Mackley,et al.  Heat transfer and associated energy dissipation for oscillatory flow in baffled tubes , 1995 .

[19]  J. Heijnen,et al.  The biofilm airlift suspension extension reactor – II: Three-phase hydrodynamics , 2000 .

[20]  T. Heindel,et al.  The effect of bubble column diameter on gas holdup in fiber suspensions , 2006 .

[21]  Estrella Alvarez,et al.  Experimental interfacial area measurements in a bubble column , 2010 .

[22]  Badie I. Morsi,et al.  Mass transfer characteristics in a large-scale slurry bubble column reactor with organic liquid mixtures , 2002 .

[23]  Xiongwei Ni,et al.  A comparative study of mass transfer in yeast for a batch pulsed baffled bioreactor and a stirred tank fermenter , 1995 .

[24]  Anh N. Phan,et al.  Effect of geometrical parameters on fluid mixing in novel mesoscale oscillatory helical baffled designs , 2011 .

[25]  M. Mackley,et al.  The axial dispersion performance of an oscillatory flow meso-reactor with relevance to continuous flow operation , 2008 .

[26]  Intermittent transition from bubbling to jetting regime in gas-liquid two phase flows , 1997 .

[27]  A P Harvey,et al.  Biological processing in oscillatory baffled reactors: operation, advantages and potential , 2013, Interface Focus.

[28]  Malcolm R. Mackley,et al.  Mixing and dispersion in a baffled tube for steady laminar and pulsatile flow , 1991 .

[29]  Nuno M. Reis,et al.  Novel oscillatory flow reactors for biotechnological applications , 2006 .

[30]  Fahir Borak,et al.  Bubble column reactors , 2005 .

[31]  J. Teixeira,et al.  Temperature and solid properties effects on gas–liquid mass transfer , 2010 .

[32]  Yoshinori Kawase,et al.  Theoretical prediction of volumetric mass transfer coefficients in bubble columns for Newtonian and non-Newtonian fluids , 1987 .

[33]  C. Bellefon,et al.  Solid effects on gas-liquid mass transfer in three-phase slurry catalytic hydrogenation of adiponitrile over raney nickel , 1996 .

[34]  Ricardo Nuno Correia Pereira,et al.  Enhanced Gas−Liquid Mass Transfer of an Oscillatory Constricted-Tubular Reactor , 2008 .

[35]  Jorge M. T. Vasconcelos,et al.  Effect of contaminants on mass transfer coefficients in bubble column and airlift contactors , 2003 .

[36]  Liang-Shih Fan,et al.  Measurement of real-time flow structures in gas–liquid and gas–liquid–solid flow systems using electrical capacitance tomography (ECT) , 2001 .

[37]  J. Teixeira,et al.  O2 mass transfer in an oscillatory flow reactor provided with smooth periodic constrictions. Individual characterization of kL and a , 2015 .

[38]  J. Teixeira,et al.  pH influence on oxygen mass transfer coefficient in a bubble column. Individual characterization of kL and a , 2013 .

[39]  Xiongwei Ni,et al.  On the determination of axial dispersion coefficient in a batch oscillatory baffled column using laser induced fluorescence , 2003 .

[40]  António A. Vicente,et al.  Residence times and mixing of a novel continuous oscillatory flow screening reactor , 2004 .

[41]  P. Stonestreet,et al.  Evaluation of a novel self‐aerating, oscillating baffle column , 1998 .

[42]  Anh N. Phan,et al.  Characterisation of mesoscale oscillatory helical baffled reactor—Experimental approach , 2012 .

[43]  J. Drahoš,et al.  Effect of viscosity on homogeneous-heterogeneous flow regime transition in bubble columns , 2003 .

[44]  J. Navaza,et al.  Mass Transfer and Influence of Physical Properties of Solutions in a Bubble Column , 2000 .

[45]  S. Paras,et al.  Effect of liquid properties on the performance of bubble column reactors with fine pore spargers , 2005 .

[46]  D. R. Skidmore,et al.  Mass transfer phenomena in an airlift reactor: Effects of solids loading and temperature , 1990, Biotechnology and bioengineering.

[47]  J. Teixeira,et al.  Hydrodynamic studies in an airlift reactor with an enlarged degassing zone , 1998 .

[48]  Pekka Oinas,et al.  Hydrodynamics and mass transfer in an airlift reactor , 1999 .

[49]  Pauline M. Doran,et al.  Bioprocess Engineering Principles , 1995 .

[50]  D. Pritchard,et al.  A study of mass transfer in yeast in a pulsed baffled bioreactor , 1995, Biotechnology and bioengineering.

[51]  P. Mena.  Mass transfer and hydrodynamics in multiphase systems , 2005 .

[52]  J. Teixeira,et al.  Proof‐of‐concept of a novel micro‐bioreactor for fast development of industrial bioprocesses , 2006, Biotechnology and bioengineering.

[53]  Raymond Lau,et al.  Bubble characteristics in shallow bubble column reactors , 2010 .

[54]  J. Teixeira,et al.  Precipitation of hydroxyapatite at 37 °C in a meso oscillatory flow reactor operated in batch at constant power density , 2013 .

[55]  K. Koide,et al.  GAS HOLDUP AND VOLUMETRIC LIQUID-PHASE MASS TRANSFER COEFFICIENT IN SOLID-SUSPENDED BUBBLE COLUMNS , 1984 .

[56]  Liang-Shih Fan,et al.  Bubble formation and dynamics in gas–liquid–solid fluidization—A review , 2007 .

[57]  M. Fialová,et al.  Duality of the gas-liquid flow regimes in bubble column reactors , 1997 .

[58]  Spiros V. Paras,et al.  Experimental study of bubble formation at metal porous spargers: Effect of liquid properties and sparger characteristics on the initial bubble size distribution , 2008 .

[59]  Chun-Min Chang,et al.  Liquid velocity and gas holdup in three-phase internal loop airlift reactors with low-density particles , 1995 .

[60]  H. Delmas,et al.  Hydrodynamics and mass transfer in bubble column: Influence of liquid phase surface tension , 2007 .

[61]  Kiyomi Akita,et al.  Bubble Size, Interfacial Area, and Liquid-Phase Mass Transfer Coefficient in Bubble Columns , 1974 .

[62]  A. Ferreira,et al.  Effect of some solid properties on gas–liquid mass transfer in a bubble column , 2011 .

[63]  António A. Vicente,et al.  Continuous-Flow Precipitation of Hydroxyapatite at 37 °C in a Meso Oscillatory Flow Reactor , 2013 .

[64]  A. Cartellier,et al.  Measurement of gas phase characteristics using a monofibre optical probe in a three-phase flow , 2008 .

[65]  Malcolm R. Mackley,et al.  AN EXPERIMENTAL INVESTIGATION INTO THE SCALE-UP OF OSCILLATORY FLOW MIXING IN BAFFLED TUBES , 2006 .

[66]  Yusuf Chisti,et al.  Mixing in Bubble Column and Airlift Reactors , 2004 .

[67]  John R. Grace,et al.  Flow regime identification in gas-liquid flow and three-phase fluidized beds , 1997 .

[68]  Yatish T. Shah,et al.  Design parameters estimations for bubble column reactors , 1982 .

[69]  Malcolm R. Mackley,et al.  Experimental fluid dispersion measurements in periodic baffled tube arrays , 1993 .

[70]  J. Dudley,et al.  Mass transfer in bubble columns: A comparison of correlations , 1995 .

[71]  Malcolm R. Mackley,et al.  Heat transfer performance for batch oscillatory flow mixing , 2002 .

[72]  José A. Teixeira,et al.  Statistical tool combined with image analysis to characterize hydrodynamics and mass transfer in a bubble column , 2012 .

[73]  António A. Vicente,et al.  Hydrodynamics of a three-phase airlift reactor with an enlarged separator : application to high cell density systems , 2008 .

[74]  H. Kikukawa,et al.  Heat transfer coefficient in bubble columns , 1981 .

[75]  Anh N. Phan,et al.  Characterisation of fluid mixing in novel designs of mesoscale oscillatory baffled reactors operating at low flow rates (0.3–0.6 ml/min) , 2011 .

[76]  X. Ni,et al.  Scale-up correlation for mass transfer coefficients in pulsed baffled reactors , 1996 .

[77]  António A. Vicente,et al.  Characterization of split cylinder airlift photobioreactors for efficient microalgae cultivation , 2014 .

[78]  Marek C. Ruzicka,et al.  EFFECT OF SOLIDS ON HOMOGENEOUS-HETEROGENEOUS FLOW REGIME TRANSITION IN BUBBLE COLUMNS , 2005 .

[79]  A. Phan,et al.  Rapid Production of Biodiesel in Mesoscale Oscillatory Baffled Reactors , 2012 .

[80]  M. C. Ruzicka,et al.  Bubble Column with Electrolytes: Gas Holdup and Flow Regimes , 2009 .

[81]  Hongbing Jian,et al.  An investigation of the effect of viscosity on mixing in an oscillatory baffled column using digital particle image velocimetry and computational fluid dynamics simulation , 2005 .

[82]  Anh N. Phan,et al.  Continuous screening of base-catalysed biodiesel production using New designs of mesoscale oscillato , 2011 .

[83]  António A. Vicente,et al.  The intensification of gas–liquid flows with a periodic, constricted oscillatory-meso tube , 2007 .

[84]  J. Teixeira,et al.  Effect of solids on gas-liquid mass transfer , 2003 .

[85]  Gabriel Wild,et al.  Measuring techniques in gas–liquid and gas–liquid–solid reactors , 2002 .

[86]  J. Teixeira,et al.  Oxygen mass transfer in a high solids loading three-phase internal-loop airlift reactor , 2001 .

[87]  Giuseppe Olivieri,et al.  Local and global hydrodynamics in a two-phase internal loop airlift , 2007 .

[88]  Emilio Molina,et al.  Studies of mixing in a concentric tube airlift bioreactor with different spargers , 1998 .