Printing of polymer microcapsules for enzyme immobilization on paper substrate.

Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.

[1]  Hong Yuan,et al.  Characteristics of paclitaxel-loaded chitosan oligosaccharide nanoparticles and their preparation by interfacial polyaddition in O/W miniemulsion system , 2010 .

[2]  Wei Li,et al.  A single-use, disposable iridium-modified electrochemical biosensor for fructosyl valine for the glycoslated hemoglobin detection , 2009 .

[3]  L. Viikari,et al.  Laccase from Melanocarpus albomyces binds effectively to cellulose , 2004, FEBS letters.

[4]  Liisa Viikari,et al.  Development of a printable laccase-based biocathode for fuel cell applications , 2008 .

[5]  D. Poncelet,et al.  Microencapsulation: fundamentals, methods and applications , 2006 .

[6]  D. Poncelet,et al.  Microencapsulation within crosslinked polyethyleneimine membranes. , 1994, Journal of microencapsulation.

[7]  C. Liu,et al.  An iridium nanoparticles dispersed carbon based thick film electrochemical biosensor and its application for a single use, disposable glucose biosensor , 2007 .

[8]  D. Rochefort,et al.  Physical immobilization of laccase on an electrode by means of poly(ethyleneimine) microcapsules , 2008 .

[9]  Dominic Rochefort,et al.  Confocal microscopy study of polymer microcapsules for enzyme immobilisation in paper substrates , 2009 .

[10]  D. Rochefort,et al.  Activity, conformation and thermal stability of laccase and glucose oxidase in poly(ethyleneimine) microcapsules for immobilization in paper , 2011 .

[11]  Ning Yan,et al.  Piezoelectric Ink‐Jet Printing of Horseradish Peroxidase: Effect of Ink Viscosity Modifiers on Activity , 2007 .

[12]  N. Hampp,et al.  Poly(methylene blue)-modified thick-film gold electrodes for the electrocatalytic oxidation of NADH and their application in glucose biosensors. , 1996, Biosensors & bioelectronics.

[13]  H. Orelma,et al.  Adsorption of different laccases on cellulose and lignin surfaces , 2009 .

[14]  Wei Shen,et al.  Thermal stability of bioactive enzymatic papers. , 2010, Colloids and surfaces. B, Biointerfaces.

[15]  Barbara Ballarin,et al.  An HRP-based amperometric biosensor fabricated by thermal inkjet printing , 2007 .

[16]  D. Rochefort,et al.  Comparison of emulsion and vibration nozzle methods for microencapsulation of laccase and glucose oxidase by interfacial reticulation of poly(ethyleneimine) , 2010, Journal of microencapsulation.

[17]  Arben Merkoçi,et al.  Configurations used in the design of screen-printed enzymatic biosensors. A review , 2000 .

[18]  L. Setti,et al.  An amperometric glucose biosensor prototype fabricated by thermal inkjet printing. , 2005, Biosensors & bioelectronics.