Rigenera® Autologous Micrografts in Oral Regeneration: Clinical, Histological, and Radiographical Evaluations

Tissue engineering represents a novel approach that aims to exploit the use of biomaterials composed mainly of scaffolds, cells (or grafts), and growth factors capable of restoring a specific tissue. Biomaterials represent the future of dental and oral regeneration due to their biocompatibility and affinity with the receiving site. The aim of this review was to collect results and considerations about a new type of biomaterial based on the use of micrografts in combination with different scaffolds. Micrografts are tissue particles enriched with progenitor cells (PCs), which are defined as descendants of stem cells that can differentiate into specialized cells belonging to the same tissue. PCs in the oral cavity might be extracted from various tissues such as dental pulp, periosteum, or periodontal ligament. Moreover, these cells are easy to isolate through a mechanical process that allows for the filtration of cells with a diameter of 80 μm, in contrast with enzymatic procedures where reagents are used and various culture periods are needed. The aim of this review was to collect data regarding the use of micrografts, developed by a Rigenera® chair-side machine, in oral regeneration evaluating the clinical, histological, and radiographical outcomes. There have been encouraging results for the application of micrografts in bone and periodontal regeneration, but further randomized clinical trials are needed to validate this promising outcome.

[1]  Antonino Lo Giudice,et al.  Evaluation of Imaging Software Accuracy for 3-Dimensional Analysis of the Mandibular Condyle. A Comparative Study Using a Surface-to-Surface Matching Technique , 2020, International journal of environmental research and public health.

[2]  A. Alibrandi,et al.  Effectiveness of a nutraceutical agent in the non-surgical periodontal therapy: a randomized, controlled clinical trial , 2020, Clinical Oral Investigations.

[3]  Vincenzo Grassia,et al.  New Materials in Oral Surgery , 2020, Materials.

[4]  A. Alibrandi,et al.  Periodontitis and Tooth Loss Have Negative Systemic Impact on Circulating Progenitor Cell Levels: A Clinical Study , 2019, Genes.

[5]  A. Alibrandi,et al.  Expression of Salivary and Serum Malondialdehyde and Lipid Profile of Patients with Periodontitis and Coronary Heart Disease , 2019, International journal of molecular sciences.

[6]  W. Cui,et al.  Advanced biomaterials for repairing and reconstruction of mandibular defects. , 2019, Materials science & engineering. C, Materials for biological applications.

[7]  S. Pierdomenico,et al.  Human Oral Stem Cells, Biomaterials and Extracellular Vesicles: A Promising Tool in Bone Tissue Repair , 2019, International journal of molecular sciences.

[8]  Giuseppe Perale,et al.  Bone grafts: which is the ideal biomaterial? , 2019, Journal of clinical periodontology.

[9]  N. Pandis,et al.  Cell therapy for orofacial bone regeneration: A systematic review and meta-analysis. , 2019, Journal of clinical periodontology.

[10]  H. De Bruyn,et al.  Biomaterials and regenerative technologies used in bone regeneration in the craniomaxillofacial region: Consensus report of group 2 of the 15th European Workshop on Periodontology on Bone Regeneration. , 2019, Journal of clinical periodontology.

[11]  A. Magi,et al.  Rationale and pre-clinical evidences for the use of autologous cartilage micrografts in cartilage repair , 2018, Journal of Orthopaedic Surgery and Research.

[12]  G. Ceccarelli,et al.  Maxillary Sinus Lift Using Autologous Periosteal Micrografts: A New Regenerative Approach and a Case Report of a 3-Year Follow-Up , 2018, Case reports in dentistry.

[13]  E. Ramos,et al.  Autologous dental pulp mesenchymal stem cells for inferior third molar post-extraction socket healing: A split-mouth randomised clinical trial , 2018, Medicina oral, patologia oral y cirugia bucal.

[14]  F. Romano,et al.  Human intrabony defect regeneration with micrografts containing dental pulp stem cells: A randomized controlled clinical trial , 2018, Journal of clinical periodontology.

[15]  N. Lang,et al.  Periodontal health , 2018, Journal of clinical periodontology.

[16]  Y. Ke,et al.  Stem cells applications in bone and tooth repair and regeneration: New insights, tools, and hopes , 2018, Journal of cellular physiology.

[17]  Shengshou Hu,et al.  Epicardial delivery of autologous atrial appendage micrografts during coronary artery bypass surgery—safety and feasibility study , 2017, Pilot and Feasibility Studies.

[18]  D. Kaigler,et al.  Stem cell therapy for reconstruction of alveolar cleft and trauma defects in adults: A randomized controlled, clinical trial , 2017, Clinical implant dentistry and related research.

[19]  G. Ceccarelli,et al.  Autologous Periosteum-Derived Micrografts and PLGA/HA Enhance the Bone Formation in Sinus Lift Augmentation , 2017, Front. Cell Dev. Biol..

[20]  Hang Cheng,et al.  Prolonged Operative Duration Increases Risk of Surgical Site Infections: A Systematic Review , 2017, Surgical infections.

[21]  C. Redi,et al.  In Vitro and In Vivo Differentiation of Progenitor Stem Cells Obtained After Mechanical Digestion of Human Dental Pulp , 2017, Journal of cellular physiology.

[22]  G. Ferraro,et al.  An innovative regenerative treatment of scars with dermal micrografts , 2016, Journal of cosmetic dermatology.

[23]  G. Ceccarelli,et al.  Osteogenic Potential of Human Oral‐Periosteal Cells (PCs) Isolated From Different Oral Origin: An In Vitro Study , 2016, Journal of cellular physiology.

[24]  Yan Jin,et al.  Treatment of periodontal intrabony defects using autologous periodontal ligament stem cells: a randomized clinical trial , 2016, Stem Cell Research & Therapy.

[25]  G. Pirozzi,et al.  A New Medical Device Rigeneracons Allows to Obtain Viable Micro‐Grafts From Mechanical Disaggregation of Human Tissues , 2015, Journal of cellular physiology.

[26]  A. Chaparro,et al.  Mesenchymal stem cells from the oral cavity and their potential value in tissue engineering. , 2015, Periodontology 2000.

[27]  C. Ferretti,et al.  Periosteum derived stem cells for regenerative medicine proposals: Boosting current knowledge. , 2014, World journal of stem cells.

[28]  G. Brunelli,et al.  Sinus lift tissue engineering using autologous pulp micro-grafts: A case report of bone density evaluation , 2013, Journal of Indian Society of Periodontology.

[29]  F. Carinci,et al.  Periodontal tissue generation using autologous dental ligament micro-grafts: Case report with 6 months follow-up , 2013 .

[30]  J. Mao,et al.  Stem cells in the face: tooth regeneration and beyond. , 2012, Cell stem cell.

[31]  P. Giannoudis,et al.  Complications following autologous bone graft harvesting from the iliac crest and using the RIA: a systematic review. , 2011, Injury.

[32]  Boon Chong Tan,et al.  Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method , 2011, Cell biology international.

[33]  L. Laino,et al.  Human mandible bone defect repair by the grafting of dental pulp stem/progenitor cells and collagen sponge biocomplexes. , 2009, European cells & materials.

[34]  Molly M. Stevens,et al.  Biomaterials for bone tissue engineering , 2008 .

[35]  E. Nkenke,et al.  Morbidity of harvesting of bone grafts from the iliac crest for preprosthetic augmentation procedures: a prospective study. , 2004, International journal of oral and maxillofacial surgery.

[36]  N. Lang,et al.  Bleeding on probing. A predictor for the progression of periodontal disease? , 1986, Journal of clinical periodontology.

[37]  F. Romano,et al.  Regenerative Treatment of Periodontal Intrabony Defects Using Autologous Dental Pulp Stem Cells: A 1-Year Follow-Up Case Series. , 2018, The International journal of periodontics & restorative dentistry.

[38]  G. Ceccarelli,et al.  Periosteum-derived micro-grafts for tissue regeneration of human maxillary bone , 2016 .

[39]  Giaccone A New Medical Device, Based on Rigenera Protocol, in the Management of Complex Wounds , 2014 .

[40]  V. Goldberg,et al.  Bone and cartilage formation in diffusion chambers by subcultured cells derived from the periosteum. , 1990, Bone.