Curcumin-polyethylene glycol loaded on chitosan- gelatin nanoparticles enhances burn wound healing in rat.

The aim of the present study was to evaluate effects of curcumin-polyethylene glycol loaded on chitosan- gelatin nanoparticles (C-PEG-CGNPs) on burn wound healing in rat in as a model study. Sixty healthy male White Wistar rats were randomized into four experimental groups of 15 animals each: Control group (Control) was treated with normal saline. Carrier group was treated with chitosan-gelatin nanoparticles based ointment (0.05 mg/mL). Silver Sulfadiazine group (SSD) was treated with silver sulfadiazine 1% ointment. Treatment group was treated with C-PEG-CGNPs (0.05 mg/mL). Wound size was measured on 7, 14 and 21 days after surgery. The expression of p53, bcl-2, Caspase III, were evaluated using reverse-transcription PCR and Immunohistochemical staining. Reduction in wound area indicated that there was significant difference between Treatment group and other groups (P < 0.05). Quantitative histological and morphometric studies, and mean rank of the qualitative studies demonstrated that there was significant difference between Treatment group and other groups (P < 0.05). Observations demonstrated C-PEG-CGNPs significantly shortened the inflammatory phase and accelerated the cellular proliferation. Accordingly, the animals in Treatment group revealed significantly (P <0.05) higher fibroblast distribution/one mm 2 of wound area and rapid re epithelialization. The mRNA levels of bcl-2, p53 and caspase III were remarkably (P <0.05) higher in Treatment group compared to control animals. The IHC analyses confirmed the RT-PCR findings. C-PEG-CGNPs offered potential advantages in burn wound healing acceleration and improvement.