Import of Carrier Proteins into Mitochondria

Abstract Carrier proteins located in the inner membrane of mitochondria are responsible for the exchange of metabolites between the intermembrane space and the matrix of this organelle. All members of this family are nuclear-encoded and depend on translocation machineries for their import into mitochondria. Recently many new translocation components responsible for the import of carrier proteins were identified. It is now possible to describe a detailed import pathway for this class of proteins. This review highlights the contribution made by translocation components to the process of carrier protein import into mitochondria.

[1]  T. Lithgow,et al.  Mas37p, a novel receptor subunit for protein import into mitochondria , 1995, The Journal of cell biology.

[2]  M. Kiebler,et al.  Import of ADP/ATP carrier into mitochondria: two receptors act in parallel , 1990, The Journal of cell biology.

[3]  G. Daum,et al.  Mrs5p, an Essential Protein of the Mitochondrial Intermembrane Space, Affects Protein Import into Yeast Mitochondria* , 1996, The Journal of Biological Chemistry.

[4]  N. Pfanner,et al.  The Import Route of ADP/ATP Carrier into Mitochondria Separates from the General Import Pathway of Cleavable Preproteins at thetrans Side of the Outer Membrane* , 1998, The Journal of Biological Chemistry.

[5]  W. Neupert,et al.  Specific recognition of mitochondrial preproteins by the cytosolic domain of the import receptor MOM72. , 1994, The Journal of biological chemistry.

[6]  S. Merchant,et al.  Human deafness dystonia syndrome is a mitochondrial disease. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[7]  N. Pfanner,et al.  Functional Staging of ADP/ATP Carrier Translocation across the Outer Mitochondrial Membrane* , 1999, The Journal of Biological Chemistry.

[8]  D. Vestweber,et al.  A yeast mitochondrial outer membrane protein essential for protein import and cell viability , 1990, Nature.

[9]  K. Mihara.  [Cytoplasmic chaperones in precursor targeting to mitochondria]. , 1996, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.

[10]  W. Neupert,et al.  MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19. , 1995, The EMBO journal.

[11]  M. Douglas,et al.  Mitochondrial import of the ADP/ATP carrier protein in Saccharomyces cerevisiae. Sequences required for receptor binding and membrane translocation. , 1988, The Journal of biological chemistry.

[12]  S. Merchant,et al.  Tim9p, an essential partner subunit of Tim10p for the import of mitochondrial carrier proteins , 1998, The EMBO journal.

[13]  M. Bauer,et al.  Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22 , 1996, Nature.

[14]  W. Neupert,et al.  The carboxyl-terminal two-thirds of the ADP/ATP carrier polypeptide contains sufficient information to direct translocation into mitochondria. , 1987, The Journal of biological chemistry.

[15]  N. Pfanner,et al.  Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway. , 1999, Molecular biology of the cell.

[16]  T. Lithgow,et al.  Acidic receptor domains on both sides of the outer membrane mediate translocation of precursor proteins into yeast mitochondria. , 1995, The EMBO journal.

[17]  M. Jacquet,et al.  The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins , 1995, Molecular and cellular biology.

[18]  N. Pfanner,et al.  Preprotein Translocase of the Outer Mitochondrial Membrane: Molecular Dissection and Assembly of the General Import Pore Complex , 1998, Molecular and Cellular Biology.

[19]  C. K. Kassenbrock,et al.  Genetic and biochemical characterization of ISP6, a small mitochondrial outer membrane protein associated with the protein translocation complex. , 1993, The EMBO journal.

[20]  H. Horstmann,et al.  Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70. , 1990, The EMBO journal.

[21]  W. Neupert,et al.  Tim9, a new component of the TIM22·54 translocase in mitochondria , 1999, The EMBO journal.

[22]  K. Dietmeier,et al.  Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins , 1998, Nature.

[23]  W. Neupert,et al.  A mitochondrial import receptor for the ADP/ATP carrier , 1990, Cell.

[24]  G. Rödel,et al.  A soluble 12-kDa protein of the mitochondrial intermembrane space, Mrs11p, is essential for mitochondrial biogenesis and viability of yeast cells , 1997, Molecular and General Genetics MGG.

[25]  N. Pfanner,et al.  Characterization of the preprotein translocase of the outer mitochondrial membrane by blue native electrophoresis. , 1996, Biological chemistry.

[26]  W. Neupert,et al.  Mitochondrial protein import. Bypass of proteinaceous surface receptors can occur with low specificity and efficiency. , 1989, The Journal of biological chemistry.

[27]  K. Dietmeier,et al.  Tom7 modulates the dynamics of the mitochondrial outer membrane translocase and plays a pathway‐related role in protein import. , 1996, The EMBO journal.

[28]  F. Palmieri.  Mitochondrial carrier proteins , 1994, FEBS letters.

[29]  W. Neupert,et al.  Transport of the ADP/ATP carrier of mitochondria from the TOM complex to the TIM22·54 complex , 1999, The EMBO journal.

[30]  C. Koehler,et al.  Import of mitochondrial carriers mediated by essential proteins of the intermembrane space. , 1998, Science.

[31]  N. Pfanner,et al.  The intermembrane space domain of mitochondrial Tom22 functions as a trans binding site for preproteins with N-terminal targeting sequences , 1997, Molecular and cellular biology.

[32]  Maithreyan Srinivasan,et al.  The Tim54p–Tim22p Complex Mediates Insertion of Proteins into the Mitochondrial Inner Membrane , 1997, The Journal of cell biology.

[33]  W. Neupert,et al.  Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5 , 1998, Nature.

[34]  K. Dietmeier,et al.  Tom5 functionally links mitochondrial preprotein receptors to the general import pore , 1997, Nature.

[35]  W. Neupert,et al.  Distinct steps in the import of ADP/ATP carrier into mitochondria. , 1987, The Journal of biological chemistry.

[36]  W. Neupert,et al.  Transport of proteins into mitochondria. Posttranslational transfer of ADP/ATP carrier into mitochondria in vitro. , 1980, European journal of biochemistry.

[37]  K. Dietmeier,et al.  Differential Recognition of Preproteins by the Purified Cytosolic Domains of the Mitochondrial Import Receptors Tom20, Tom22, and Tom70* , 1997, The Journal of Biological Chemistry.

[38]  N. Pfanner,et al.  Targeting and assembly of the oxoglutarate carrier: general principles for biogenesis of carrier proteins of the mitochondrial inner membrane. , 1998, The Biochemical journal.

[39]  J. Schneider-Mergener,et al.  Distribution of Binding Sequences for the Mitochondrial Import Receptors Tom20, Tom22, and Tom70 in a Presequence-carrying Preprotein and a Non-cleavable Preprotein* , 1999, The Journal of Biological Chemistry.