Molecular characterization of a phytoplasma of group 16SrIX related to 'Ca. Phytoplasma phoenicium' in periwinkle in Brazil

Periwinkle (Catharanthus roseus), a tropical perennial plant, was found to be infected by a phytoplasma. Plants exhibiting virescence, phyllody and variegation symptoms were collected in the states of Minas Gerais and Sao Paulo, Brazil. The phytoplasma was transmitted by grafting from an infected periwinkle plant to healthy plants and by dodder to a citrus plant. Phytoplasma isolates from periwinkle plants from Brazil had the 16S rDNA gene sequenced and were classified in the 16SrIX group, subgroup A, belonging to the 'Candidatus P. phoenicium' species.

[1]  Chan-Pin Lin,et al.  Characterization of Catharanthus roseus Genes Regulated Differentially by Peanut Witches’ Broom Phytoplasma Infection , 2011 .

[2]  V. Carginale,et al.  Gene expression profiling of phytoplasma-infected Madagascar periwinkle leaves using differential display , 2011, Molecular Biology Reports.

[3]  Wei Wei,et al.  Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII) , 2009, International journal of systematic and evolutionary microbiology.

[4]  E. Kitajima,et al.  A phytoplasma closely related to the pigeon pea witches'-broom phytoplasma (16Sr IX) is associated with citrus huanglongbing symptoms in the state of São Paulo, Brazil. , 2008, Phytopathology.

[5]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.

[6]  J. Aguirreolea,et al.  Purification, molecular cloning, and cell-specific gene expression of the alkaloid-accumulation associated protein CrPS in Catharanthus roseus. , 2005, Journal of experimental botany.

[7]  R. Davis,et al.  First Report of Natural Infection by "Candidatus Phytoplasma brasiliense" in Catharanthus roseus. , 2001, Plant disease.

[8]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[9]  D. Gundersen-Rindal,et al.  Revised Classification Scheme of Phytoplasmas based on RFLP Analyses of 16S rRNA and Ribosomal Protein Gene Sequences , 1998 .

[10]  Ing-Ming Lee,et al.  Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. , 1996 .

[11]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[12]  James B. Hicks,et al.  A plant DNA minipreparation: Version II , 1983, Plant Molecular Biology Reporter.

[13]  A. Bertaccini.  Phytoplasmas: diversity, taxonomy, and epidemiology. , 2007, Frontiers in bioscience : a journal and virtual library.

[14]  P. Weintraub,et al.  Insect vectors of phytoplasmas. , 2006, Annual review of entomology.

[15]  B. Schneider,et al.  'Candidatus Phytoplasma', a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. , 2004, International journal of systematic and evolutionary microbiology.

[16]  R. O. Resende,et al.  DIVERSIDADE DE ISOLADOS BRASILEIROS DE FITOPLASMAS ATRAVES DA ANALISE DO 16S RDNA , 1998 .

[17]  Gapped BLAST and PSI-BLAST: A new , 1997 .

[18]  Bernd Schneider,et al.  Taxonomic relatedness and phylogenetic positions of phytoplasmas associated with diseases of faba bean, sunnhemp, sesame, soybean, and eggplant , 1995 .